Isolation and cloning by a polymerase chain reaction of a genomic DNA fragment of the human slow skeletal troponin (TNNT1) gene

Cell Biochemistry and Function

Volumn 11, Issue 3, 1993, Pages 187-191

  Novelli, G ^a   Gennarelli, M ^b   Sangiuolo, F ^b   D'Agruma, L ^b   Cicero, S Lo ^b   Melchionda, S ^a   Dallapiccola, B ^a,c  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

^c CASA SOLLIEVO DELLA SOFFERENZA HOSPITAL   (Italy)

Author keywords

Alu sequences; Polymerase chain reaction; troponin T

Indexed keywords

DNA FRAGMENT; TROPONIN T;

ARTICLE; CLONING; CONTROLLED STUDY; GENE ISOLATION; INTRON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SKELETAL MUSCLE;

BASE SEQUENCE; CHROMOSOMES, HUMAN, PAIR 19; CLONING, MOLECULAR; GENOMIC LIBRARY; HUMAN; INTRONS; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, NUCLEIC ACID; SEQUENCE ANALYSIS, DNA; SUPPORT, NON-U.S. GOV'T; TROPONIN; TROPONIN T;

EID: 0027204286     PISSN: 02636484     EISSN: 10990844     Source Type: Journal    
DOI: 10.1002/cbf.290110306     Document Type: Article

References (19)

1. Intricate combinatorial patterns of exon splicing generate multiple regulated troponin T isoforms from a single gene
2. Alternative splicing generates variants in important functional domains of human slow skeletal troponin T
3. Isolation and characterization of a slow troponin T (TnT) gene on chromosome 19 by substraction hybridization of a cDNA muscle library using myotonic dystrophy muscle cDNA
4. Assignment of the slow troponin T (TNNT1) gene to chromosome 19 using polymerase chain reaction
5. Developmentally induced, muscle‐specific trans factors control the differential splicing of alternative and costitutive troponin T exons
6. A single cardiac troponin T gene generates embryonic and adult isoforms via developmentally regulated alternative splicing
7. Polymerase chain reaction in the detection of mRNA transcripts from the slow skeletal troponin T (TNNT1) gene in myotonic dystrophy and normal muscle
8. Novel non‐templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases
9. Generation of a single strand by polymerase chain reaction and its application to direct sequencing
10. Structure and variability of recently inserted Alu family members
11. Cloning, structural analysis, and expression of the human fast twitch skeletal muscle troponin C gene
12. Tissue‐specific heterogeneity of the 3′‐untranslated region of L‐type pyruvate kinase mRNAs
13. Isolation and characterization of the human pyruvate kinase M gene
14. Cloning, structural analysis, and expression of the human slow twitch skeletal muscle/cardiac troponin C gene
15. The lipoprotein lipase‐encoding human gene: sequence from intron‐6 to intron‐9 and presence in intron‐7 of a 40 million‐year‐old Alu sequence
16. The human LDL receptor: a cysteine‐rich protein with multiple Alu sequences in its mRNA
17. Analysis of a gene cluster coding for tRNA GAAPhe and tRNA UUULys
18. Simple sequences are ubiquitous repetitive components of eukaryotic genomes