Crystallization of a 67 kDa fragment of Escherichia coli DNA topoisomerase I

Journal of Molecular Biology

Volumn 232, Issue 4, 1993, Pages 1213-1216

  Lima, Christopher D ^a   Wang, James C ^b   Mondragón, Alfonso ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

Crystallization; DNA cleavage; DNA topoisomerases; E. coli DNA topoisomerase I; X ray crystallography

Indexed keywords

DNA TOPOISOMERASE; PROTEIN FRAGMENT; UNCLASSIFIED DRUG;

ARTICLE; CRYSTALLIZATION; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; X RAY ANALYSIS;

CRYSTALLIZATION; DNA TOPOISOMERASES, TYPE I; ESCHERICHIA COLI; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; SUPPORT, U.S. GOV'T, P.H.S.; X-RAY DIFFRACTION;

ESCHERICHIA COLI;

EID: 0027179285     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1993.1474     Document Type: Article

References (31)

1. Andoh, T., Ikeda, H. & Oguro, M. (eds) (1993). Molecular Biology of DNA topoisomerases and its applications to chemotherapy. In Proceedings of the International Symposium on DNA Topoisomerases in Chemotherapy. Nagoya, Japan, CRC Press, Inc., Boca Raton, FL 1993.
2. Beran-Steed, T. R. & Tse-Dinh, Y.-C. (1989). The carboxyl terminal domain of Esckerichia coli DNA topoisomerase I confers higher affinity to DNA. Proteins: Struct. Fund. Genet. 2, 249-258
3. Brown, P. O. & Cozzarelli, N. R. (1981). Catenation and knotting of duplex DNA by type 1 toposiomerases; a mechanistic parallel with type 2 topoisomerases. Proc. Nat. Acad. Sei., U.S.A. 78, 843-847.
4. Confalonieri, F., Ehe, C., Nadal, M., Bouthier de La Tour, C., Forterre, P. & Duguet, M. (1993). Reverse gyrase: a helicase-like domain and a type I topoisomerase in the same polypeptide Proc. Nat. Acad. Sei., U.S.A. 90, 4753-4757.
5. Dean, F., Krasnow, M. A., Otter, R., Matzuk, M. M., Spengler, S. J. & Cozzarelli, N. R, (1983). Escherichia coli type-I topoisomerases: identification, mechanism, and role in recombination. Cold Spring Harbor Symp. Quant. Biol. 47, 769-784.
6. DiGate, R. & Marians, K. (1989). Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III. J. Biol. Chem. 264, 17924-17930.
7. Geliert, M. (1981). DNA topoisomerases. Annu. Rev. Biochem. 50, 879-910.
8. Hope, H. (1988). Cryocrystallography of biological macro-molecules: a generally applicable method. A eta Cryslallogr. sect. B, 44, 22-26.
9. Jancarik, J. & Kim, S.-H. (1991). Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryslallogr. 24, 409-411.
10. Kim, R. A. & Wang, J, C. (1992). Identification of the yeast TOP3 gene product as a single strand-specific DNA topoisomerase. J. Biol. Chem. 267, 17178-17185.
11. Kirkegaard, K. & Wang, J. C- (1978). Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequences. Nucl. Acids. Res. 5, 3811-3820.
12. Kirkegaard, K. & Wang, J. C. (1985). Bacterial DNA topoisomerase I can relax positively supercoiled DNA containing a single-stranded loop. J. Mol. Biol. 185, 625-637.
13. Liu, L. F. (1989). DNA topoisomerases poisons as antitumor drugs. Annu. Rev. Biochem. 58, 351-375.
14. Liu, L. F., Depew, R. E. & Wang, J. C. (1976). Knotted single-stranded DNA rings: a novel topological isomer of circular single-stranded DNA formed by treatment with Escherichia coli ω protein. J. Mol. Biol. 106, 439-452.
15. Low, R. L., Kaguni, J. M. & Kornberg, A. (1984). Potent catenation of supercoiled and gapped DNA circles by topoisomerase 1 in the presence of a hydrophilic polymer. J. Biol. Chem. 259, 4576-4581.
16. Lynn, R. & Wang, J. C. (1989). Peptide sequencing and site-directed mutagenesis identify tyrosine-319 as the active site tyrosine of Escherichia coli DNA topoisomerase I. Proteins: Struct. Fund, Genet. 6, 231-239.
17. Margolin, P., Zumstein, L., Sternglanz, R. & Wang, J. C. (1985). The Escherichia coli supX locus is top A, the structural gene for DNA topoisomerase I. Proc. Nat. Acad. Sei., U.S.A. 82, 5437-5441.
18. Matthews, B. W. (1968). Solvent content of protein crystals. J. Mol. Biol. 33, 491-497.
19. Smith, R. H. & Johnson, K. S. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene, 67, 31-40.
20. Sternglanz, R., DiNardo, S., Voelkel, K. A., Nishimura, Y., Hirota, Y., Beeherer, K., Zumstein, L. & Wang, J. C. (1981). Mutations in the gene coding for Escherichia call DIVA topoisornerase I affect transcription and transposition. Proc. Nat. Acad. Sei., U.S.A. 78, 2747-2751.
21. Teng, T.-Y. (1990). Mounting of crystals for macro-molecular crystallography in a free-standing thin film. J. Appl. Crystallogr. 23. 387-391.
22. Tse-Dinh, Y.-C. (1991). Zine(III) coordination in Escherichia cod ON A topoisornerase Γ is required for cleavable complex formation with DNA. J. Biol. Chem. 266, 14317-14320.
23. Tse, Y.-C. & Wang, I. C. (1980). E. cod and M. luteus DNA topoisornerase I can catalyze catenation or decatenation of double stranded ON A rings. Cell, 22, 209-276.
24. Tse-Dinh, Y.-C. & Wang, J. C. (1986). Complete nucleotide sequence of the lop A gene encoding Escherichia coli DNA topoisornerase T. J. Mol. Biol. 191, 321-331.
25. Tse-Dinh, Y.-C., McCarron, B. G. H., Arntzen, R. & Chowdhry, V. (1986). Mechanistic study of E. coli DNA topoisornerase I: cleavage of oligonucleotides. Nucl. Acids Res. 11, 8691-8701.
26. Wallis, J. W., Chrebet, G., Brodsky, G., Rolfe, M. & Roth stein, R. (1989). A hyper-recombination mutation in S. cerevisiae. identifies a novel eukaryotic topoisornerase. Cell, 58, 409-419.
27. Wang, J. C. (1971). Interaction between DNA and an Escherichia coli protein ω. J. Mol. Biol. 55, 523-533.
28. Wang, J. C. (1985), DNA topoisornerase. Annu. Rev. Biochem. 54, 665-697.
29. Wang, J. C. (1991). DNA topoisomerases: why so many? J. Biol. Chem. 266, 6659-6662.
30. Wang, J. C. & Beeherer, K. (1983). Cloning of the gene topA encoding for DNA topoisornerase T and the physical mapping of the cysB-topA -trp region of Escherichia coli. Nucl. Acids Res. 11, 1773-1790.
31. Zumstein, L. & Wang, J. C. (1986), Probing the structural domains and function in vivo of Escherichia coli DNA topoisornerase I by mutagenesis. J. Mol. Biol. 191, 333-340