Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria

Gene

Volumn 127, Issue 1, 1993, Pages 15-21

  Quandt, Jürgen ^a   Hynes, Michael F ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

'suicide' plasmids; cosmids; gentamicin resistance; insertional mutagenesis; Reverse genetics; Rhizobium; sacB

Indexed keywords

DNA;

ARTICLE; BACILLUS; ESCHERICHIA COLI; GENOME; MUTAGENESIS; PLASMID; PRIORITY JOURNAL; VECTOR CONTROL;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; COSMIDS; DNA, BACTERIAL; DRUG RESISTANCE, MICROBIAL; GENES, BACTERIAL; GENES, LETHAL; GENETIC VECTORS; GENTAMICINS; GRAM-NEGATIVE BACTERIA; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PLASMIDS; RHIZOBIUM LEGUMINOSARUM; SUPPORT, NON-U.S. GOV'T;

BACILLUS SUBTILIS; BACTERIOPHAGE LAMBDA; ENTEROBACTERIA; ESCHERICHIA COLI; NEGIBACTERIA; RHIZOBIUM LEGUMINOSARUM;

EID: 0027158657     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(93)90611-6     Document Type: Article

References (24)

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7. A small cosmid for efficient cloning of large DNA fragments
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15. Genes involved in lipopolysaccharide production and symbiosis are clustered on the chromosome of Rhizobium leguminosarum biovar viciae VF39
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23. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites
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