Herbicide-resistant Indica rice plants from IRRI breeding line IR72 after PEG-mediated transformation of protoplasts

Plant Molecular Biology

Volumn 20, Issue 4, 1992, Pages 619-629

  Datta, Swapan K ^a   Datta, Karabi ^a   Soltanifar, Nouchine ^a   Donn, Gunter ^b   Potrykus, Ingo ^a  

^a ETH ZURICH   (Switzerland)

^b SANOFI   (France)

Author keywords

herbicide resistance; hygromycin phosphotransferase; Indica rice; IRRI breeding line; PEG mediated protoplast transformation; phosphinothricin acetyltransferase

Indexed keywords

AMINOBUTYRIC ACID DERIVATIVE; BUFFER; HERBICIDE; HYGROMYCIN B; HYGROMYCIN B KINASE; HYGROMYCIN-B KINASE; MACROGOL DERIVATIVE; PHOSPHINOTHRICIN; PHOSPHOTRANSFERASE;

ARTICLE; CELL CULTURE; DRUG RESISTANCE; EMBRYOLOGY; GENETIC TRANSFORMATION; GENETICS; METABOLISM; MOLECULAR CLONING; PHYSIOLOGY; PLASMID; REGENERATION; RICE; SOUTHERN BLOTTING; TRANSGENIC PLANT;

AMINOBUTYRIC ACIDS; BLOTTING, SOUTHERN; BUFFERS; CELLS, CULTURED; CLONING, MOLECULAR; DRUG RESISTANCE; HERBICIDES; HYGROMYCIN B; ORYZA SATIVA; PHOSPHOTRANSFERASES; PLANTS, GENETICALLY MODIFIED; PLASMIDS; POLYETHYLENE GLYCOLS; REGENERATION; SUPPORT, NON-U.S. GOV'T; TRANSFORMATION, GENETIC;

EID: 0026951881     PISSN: 01674412     EISSN: 15735028     Source Type: Journal    
DOI: 10.1007/BF00046447     Document Type: Article

References (52)

1. Histochemical analysis of CaMV promoter β-glucuronidase gene expression in transgenic rice plants
2. The 3′-terminal region of the hygromycin B resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum
3. Hygromycin B phospho-transferase as a selectable marker for DNA transfer experiments with higher eucarytic cells
4. Engineering herbicide resistance in plants by expression of a detoxifying enzyme
5. Production of transgenic rice (Oryza sativa) plants from agronomically important Indica and Japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos
6. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources
7. Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa)
8. Genetically engineered fertile Indica-rice recovered from protoplasts
9. Transgenic rice. Characterization of protoplast derived plants and their seed progeny
10. Direct DNA transfer to plant cells
11. Evaluation of selectable markers for rice transformation
12. Stable transformation of maize with a chimaeric, modified phophinothricinacetyl transferase gene from Streptomyces virido-chromogenes
13. A technique for radio labelling DNA restriction fragments to high specific activities
14. Inheritance and expression of chimeric genes in the progeny of transgenic maize
15. Stable transformation of maize after gene transfer by electroporation
16. Genetically engineering plants for crop improvement
17. Transformation of maize cells and regeneration of fertile transgenic plants
18. A PEG mediated protoplast transformation system for production of fertile transgenic rice plants
19. Transformation of plant cells via Agro-bacterium
20. Agricultural progress: engineering crop species, field trial results and commercialization issues
21. In vitro transformation of plant protoplasts with Ti plasmid DNA
22. Tissue specific expression of the rolC promoter of the Ri plasmid in transgenic rice plants
23. Transgenic rice cell lines and plants: expression of transferred chimeric genes
24. Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase
25. A revised medium for a rapid growth and bioassay with tobacco tissue culture
26. Rapid isolation of high molecular weight plant DNA
27. Hybrid genes in the analysis of transformation conditions
28. Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture
29. Direct gene transfer to plants
30. Plant gene engineering and plant agriculture
31. Recognition efficiency of Dicotyledoneae specific promoter and RNA processing signals in rice
32. Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector
33. Gene transfer to cereals. An assessment
34. Gene transfer to plants: assessment of published approaches and results
35. Direct gene transfer to cells of graminaceous monocot
36. Genetically transformed maize plants from protoplasts
37. Transgenic plants as tools to study the molecular organisation of plant genes
38. Agarose plating and a bead type culture techniques enable and stimulate development of protoplast derived colonies in a number of plant species
39. Fertile transgenic rice plants regenerated from transformed protoplasts
40. Detection of specific sequences among DNA fragments separated by gel electrophoresis
41. Bialaphos selection of stable transformants from maize cell cultures
42. Segregation of transgenes in maize
43. Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions
44. Expression of a monocot LHCP promoter in transgenic rice
45. Transgenic rice plants after direct gene transfer into protoplasts
46. Developing cell and tissue cultures system for the improvement of cereals and grass crops
47. Plant tissue culture and molecular biology as tools in understanding plant development and in plant improvement
48. Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants
49. Analysis of rice Act1 5′ region activity in transgenic rice plants
50. Potentially useful genes for rice genetic engineering