Sensitivity of detection of heterozygous point mutations in p53 cDNAs by direct PCR sequencing

Genome Research

Volumn 1, Issue 3, 1992, Pages 199-201

  Cheng, Jian ^a   Haas, Martin ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ALLELE; ARTICLE; GENETICS; HUMAN; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TUMOR SUPPRESSOR GENE;

ALLELES; DNA; DNA MUTATIONAL ANALYSIS; EVALUATION STUDIES; GENES, P53; HUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.;

EID: 0026816907     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.1.3.199     Document Type: Article

References (10)

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3. Myers, R.M., Z. Larin, and T. Maniatis. 1985. Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science 230: 1242-1246.
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8. Suzuki, Y., M. Orita, M. Shiraishi, K. Hayashi, and T. Sekiya. 1990. Detection of ras gene mutations in human lung cancers by single-strand conformation polymorphism analysis of polymerase chain reaction products. Oncogene 5: 1037-1043.
9. Gyllensten, U.B. and H.A. Erlich. 1988. Generation of single stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc. Natl. Acad. Sci. 85: 7652-7656.
10. Zakut-Houri, R., B. Bienz-Tadmor, D. Givol, and M. Oren. 1985. Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4: 1251-1255.