Immunological Quantitation of Thymidylate Synthase Using the Monoclonal Antibody TS 106 in 5-Fluorouracil-sensitive and -resistant Human Cancer Cell Lines

Cancer Research

Volumn 52, Issue 16, 1992, Pages 4306-4312

  Johnston, Patrick G ^a   Drake, James C ^a   Trepel, Jane ^a   Allegra, Carmen J ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLOXURIDINE PHOSPHATE; FLUOROURACIL; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY TS 106; THYMIDYLATE SYNTHASE; TUMOR MARKER; UNCLASSIFIED DRUG;

ARTICLE; AUTORADIOGRAPHY; BREAST CANCER; CANCER CELL CULTURE; CANCER DIAGNOSIS; CELL LYSATE; COLON CARCINOMA; DENSITOMETRY; DRUG RESISTANCE; DRUG SENSITIVITY; ENZYME ACTIVITY; ENZYME BINDING; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; PRIORITY JOURNAL; SOLID TUMOR;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; BREAST NEOPLASMS; COLONIC NEOPLASMS; DOXORUBICIN; DRUG RESISTANCE; FLUORESCENT ANTIBODY TECHNIQUE; FLUORODEOXYURIDYLATE; FLUOROURACIL; HUMAN; MOLECULAR WEIGHT; STOMACH NEOPLASMS; THYMIDYLATE SYNTHASE; TUMOR CELLS, CULTURED;

EID: 0026795489     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Santi, D. V., and Danenberg, P. V. Folates in pyrimidine nucleotide biosyn thesis. In: R. L. Blakley and S. J. Benkovic (eds.). Folates and Pterins, pp 345-398. New York: Wiley, 1984.
2. Heidelberger, C. Fluorinated pyrimidines and their nucleosides. In: A. C Sartorelli and D. G. Johns (eds.). Handbook of Experimental Pharmacology Vol. 38, pp. 193-231. New York: Springer-Verlag, 1975.
3. Danenberg, P. V. Thymidylate synthase: a target enzyme in cancer chemotherapy. Biochim. Biophys. Acta, 473: 73-92, 1977.
4. Santi, D. V., McHenry, C. S., and Sommer, M. Mechanisms of interaction of thymidylate synthase with 5-fluorodeoxyuridylate. Biochemistry, 13: 471-480, 1974.
5. Moertel, C. G. Current concepts in cancer: chemotherapy of gastrointestinal cancer. N. Engl. J. Med., 299: 1049-1052, 1978.
6. Hardy, L. W., Finer-Moore, J., Montfort, W., Jones, M., Santi, D. V., and Stroud, R. M. Atomic structure of thymidylate synthase: target for rational drug design. Science (Washington DC), 235:448-453, 1987.
7. Chu, F. K., Maley, F., and Belfort, M. Intervening sequence in the thymidylate synthase gene of bacteriophage T4. Proc. Natl. Acad. Sci. USA, 81: 3049-3053, 1984.
8. Belfort, M., Maley, G., Lane, J. P., and Maley, F. Primary structure of Escherichia coli thy A gene and its thymidylate synthase product. Proc. Natl. Acad. Sci. USA, 80:4914-4918, 1983.
9. Beverley, S. M., Ellenberger, T. E., and Cordingly, J. S. Primary structure of the gene encoding the bifunctional dihydrofolate reduction-thymidylate synthase of Leishmania major. Proc. Natl. Acad. Sci. USA, 83: 2584-2589, 1986.
10. Conrad, A. H., and Ruddle, F. H. Regulation of thymidylate synthase activity in cultured mammalian cells. J. Cell. Sci., 10: 471-486, 1972.
11. Navalbund, L. G., Rossann, C., Muench, A. J., and Johnson, L. F. Cell cycle regulation of thymidylate synthase gene expression in cultured mouse fibroblasts. J. Biol. Chem., 255: 7386-7390, 1980.
12. Johnson, L. F. Expression of dihydrofolate reductase and thymidylate synthase in mammalian cells. In: G. S. Stein and J. L. Stein (eds.). Recombinant DNA and Cell Proliferation, pp. 25-47. New York: Academic Press, 1984.
13. Jenh, C. H., Rao, L. G., and Johnson, L. F. Regulation of thymidylate synthase enzyme synthesis in 5-fluorodeoxyuridine resistant mouse fibroblasts during the transition from the resting to growing state. J. Cell. Physiol., 122: 149-154, 1985.
14. Berger, S. H., Chung-Her, J., Johnson, L. F., and Berger, F. G. Thymidylate synthase overproduction and gene amplification in fluorodeoxyuridine-resis-tant human cells. Mol. Pharmacol., 28: 461-467, 1987.
15. Clark, J. L., Berger, S. L., Mittelman, A., and Berger, F. G. Thymidylate synthase gene amplification in a colon tumor resistant to fluoropyrimidine chemotherapy. Cancer Treat. Rep., 71: 261-265, 1987.
16. Spears, C. P., Antranik, A. H., Moran, R. G., Heidelberger, C., and Corbett, T. H. In vivo kinetics of thymidylate synthase inhibition in 5-fluorouracil sensitive and resistant murine colon adenocarcinomas. Cancer Res., 42:450-456, 1982.
17. Swain, S. M., Lippman, M. E., Egan, E. F., Drake, J. C., Steinberg, S. M., and Allegra, C. J. Fluorouracil and high-dose leucovorin in previously treated patients with metastatic breast cancer. J. Clin. Oncol., 7: 890-896, 1989.
18. Spears, C. P., Gustavsson, B. G., Berne, M., Frosling, R., Bernstein, L., and Hayes, A. A. Mechanisms of innate resistance to thymidylate synthase inhibition after 5-fluorouracil. Cancer Res., 48: 5894-5900, 1988.
19. Washtein, W. L. Increased levels of thymidylate synthase in cells exposed to 5-fluorouracil. Mol. Pharmacol., 25: 171-177, 1984.
20. Chu, E., Zinn, S., Boarman, D., and Allegra, C. J. The interaction of 7 interferon and 5-fluorouracil in the H630 human colon carcinoma cell line. Cancer Res., 50: 5834-5840, 1990.
21. Lockshin, A., and Danenberg, P. V. Biochemical factors affecting the tightness of 5-fluorodeoxyuridylate binding to human thymidylate synthase. Biochem. Pharmacol., 30: 247-257, 1981.
22. Lockshin, A., and Danenberg, P. V. Thymidylate synthase and 2-deoxyuridylate form a tight complex in the presence of pteroyltriglutamate. J. Biol. Chem., 254: 12285-12288, 1979.
23. Moran, R. G., Spears, C. P., and Heidelberger, C. Biochemical determinants of tumor sensitivity to 5-fluorouracil: ultrasensitive methods for the determination of 5-fluoro-2'-deoxyuridylate and thymidylate synthase. Proc. Natl. Acad. Sci. USA, 76: 1456-1460, 1979.
24. Johnston, P. G., Liang, C. M., Henry, S., Chabner, B. A., and Allegra, C. J. The production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissues. Cancer Res., 51: 6668-6677, 1991.
25. Park, J. G., Oie, H. K., Sugarbaker, P. H., Henslee, J. G., Chen, T. R., Johnson, B. E., and Gazdar, A. Characterization of cell lines established for human colorectal carcinoma. Cancer Res., 47: 6710-6718, 1987.
26. Brattain, M. G., Fine, W. D., Khaled, F. M., Thompson, J., and Brattain, D. E. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res., 41: 1751-1756, 1981.
27. Soule, H. D., Vasquez, J., Long, A., Albert, S., and Brennan, M. A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst., 51: 1409-1416, 1973.
28. Chu, E., Drake, J. C., Koeller, D. M., Zinn, S., Jamis-Dow, C. A., Yeh, G. C., and Allegra, C. J. Induction of thymidylate synthase associated with multidrug resistance in human breast and colon cancer cell lines. Mol. Pharmacol., 39: 136-143, 1991.
29. Barranco, S. C., Townsend, C. M., Casartelli, C., Malik, B. C., Burger, N. L., Boerwinkle, W. R., and Gourley, W. K. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res., 43: 1703-1709, 1983.
30. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254, 1976.
31. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 277: 680-685, 1970.
32. Hsu, S. M., Raine, L., and Fanger, H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. J. Histochem. Cytochem., 29: 577-585, 1981.
33. Hsu, S. M., Raine, L., and Fanger, H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am. J. Clin. Pathol., 75: 734-738, 1981.
34. Tubiana, M., Pejovic, M. H., Chavaudra, N., Contesso, G., and Malaise, E. P. The long-term prognostic significance of the thymidine labelling index in breast cancer. Int. J. Cancer, ii; 441-445, 1984.
35. Silvestrini, R., Dardone, M. G., and Gasparini, G. Cell kinetics as a prognostic marker in node negative breast cancer. Cancer (Phila.), 56:1982-1987, 1985.
36. Meyer, J. S., and Hixon, B. Advanced stage and early relapse of breast carcinomas associated with high thymidine labeling indices. Cancer Res., 39: 4042-4047, 1979.
37. Bauer, K. D., Lincoln, S. T., Vera-Roman, J. M., Wallemark, C. B., Chmiel, J. S., Madurski, M. L., Murad, T., and Scarpelli, D. G. Prognostic implications of proliferative activity and DNA aneuploidy in colonic adenocarcinomas. Lab. Invest., 56: 329-334, 1987.