Foreign gene expression in yeast: a review

Yeast

Volumn 8, Issue 6, 1992, Pages 423-488

  Romanos, Michael A ^a   Scorer, Carol A ^a   Clare, Jeffrey J ^a  

^a WELLCOME RESEARCH LABORATORIES   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EXPRESSION VECTOR; GENE EXPRESSION; GENE TRANSFER; GENETIC TRANSCRIPTION; NONHUMAN; PROTEIN SECRETION; REVIEW; YEAST;

AMINO ACID SEQUENCE; DNA, RECOMBINANT; GENE EXPRESSION REGULATION, FUNGAL; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; YEASTS;

EID: 0026778048     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.320080602     Document Type: Article

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43. New expression vectors for the fission yeast Schizosaccharomyces pombe
44. Expression of human antithrombin III in Saccharomyces cerevisiae and schizosaccharomyces pombe
45. Messenger RNA stability in yeast
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48. Selection and stability of yeast transformants expressing cDNA of an M1 Killer toxin‐immunity gene
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50. RNA processingin vitor produces mature 3′ends of a variety of Saccharomyces cerevisiae mRNAs
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52. Yeast DNA replication
53. Glycosylation and structure of the yeast MFa1 α‐factor precursor is important for efficient transport through the secretory pathway
54. A new means of inducibly inactivating cellular protein
55. Genetic analysis of partitioning functions encoded by the 2μm circule of Saccharomyces cerevisiae
56. D Xylose fermentation to ethanol by Schizosaccharomyces pombe cloned with xylose isomerase gene
57. Saccharomyces cerevisiae secretes and correctly processes invertase signal peptides
58. Purification and characterisation of a methionine aminopeptidase from Saccharomyces cerevisiae
59. Synthesis of tetanus toxin frogemnt C in insect cells by use of a baculovirus expression system
60. Selection of lys2 mutants ofthe yeast Saccharomyces cerevisiae by the utilisation of α‐aminoadipate
61. Homologous versus heterologous gene expression in the yeast, Sacchoromyces cerevisiae
62. Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase
63. The host range of the pKD1‐derived plasmids in yeast
64. Sequence organisation of the circular plasmid pKD1 from the yeast Kluyveromyces drosophilarum
65. Extracellular RNase produced by Yarrowia Lipolytica
66. A novel class of vector for yeast transformation
67. Molecular and Genetic approach to enhancing protein secretion
68. Construction of multicopy yeast plasmids with regulated centromere fuction
69. Sequence and structural features associated with translationnal initiator regions in yeast—a review
70. Mutational analysis of the HIS4 translational initiator region in saccharomyces cerevisiae
71. Deletion of plasmid sequences during Saccharomyces cerevisiae transformation
72. High level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integratons of the gene
73. Production of mouse epidermal growth factor in yeast: high‐level secretion using Pichia pastoris strains containing multiple gene copies
74. Isolation of a yeast centromere and construction of fuctinal small circular chromosomes
75. Oxidation of methanol bythe yeast Pichia Pastoris. Purfication and properties of alcohol oxidase
76. Construction of a regulated PGk expressin vector
77. High level expresson of proinsulin in the yeast, Saccharomyces cerevisiae
78. Pichia pastoris as a host system for transformations
79. Development of transformation systmes and construction of methanol‐utilisation‐defective mutants of Pichia pastoris by gene disruption
80. High‐level exrpession and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast
81. Influence of dilution rate and induction of cloned gene expression in continuous fermentatons of recombinant yeast
82. Heritable damage to yeast caused by transformation
83. A high‐frequency transformaton system based on the yeast Kluyveromyces lactis
84. Integrative transformation of the yeast Yarrowia lipolytica
85. Cloning and sequencing of the alkaline extraccellular protesase gene of Yarrowia lipolytica
86. Single chain antibody (SCA) encoding genes: ne step construction and expression in eukaryotic cells
87. Fermentation of a yeast producing A.niger glucose exidase: Scale‐up, pruification and characterisation of the recombinant enzyme
88. Transformation of Kluyveromyces lactis by killer plasmid DNA
89. Efficient synthesis of secreted murine interleukin‐2 by Saccharomyces cerevisiae: influence of 3′‐untranslated regions and codon usage
90. Passage of molecules through yeast cell walls: a brief essay‐review
91. Non‐specific acid phosphatase from Schizosacchoromyces pombe. Purification and physical chemical properties
92. Molecular determinatnts of protein half‐lives in eukaryotic cells
93. The lactosegalactose regulon of Kluyveromyces lactis
94. Continuous producton of a novel lysosyme via secreton from the yeast, Pichia pastoris
95. Control of recombination within and between DNA plasmids of Saccharomyces cerevisiae
96. Loss of 2μm DNA from Saccharomyces cerevisiae transformed withthechimaeric plasmid pJDB219
97. Incressing gene expression in yeast by fusion to ubiquitin
98. A novel aspartyl protease allowing KEX2‐independent MFα propheromone processing in yeast
99. Direct enzymatic assay for alcohol oxidase, alcohol dehydrogenase and formaldehyde dehydrogenase in colonies of Hansenula polymorpha
100. Methanol metabolism in yeasts: Regulation of the synthesis of catabolite enzymes
101. The yeast regulatory protein ADRI binds in a zind‐dependent manner to the upwtream activating sequence of
102. Secretion of glycosylated human erythropoetin from yeast directed by the α‐factor leader region
103. Two other methanol regulatable genes from the yeast Pichia pastoris
104. Cap‐independent translation of mRNA conferred by encephalomyocarditis virus 5′ sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system
105. The presence of adefective LEU2 gene in 2μm DNA recombinant plasminds of Saccharomyces cerevisiae is responsible for curing and high copy number
106. Improved secretion of heterologous proteins by Saccharomyces cerevisiae: effects of promoter substitution in alpha‐factor fusions
107. Efficient secretion and processing of heterologous proteins in Saccharomyces cerevisiae is mediated solely by the pre‐segment of α‐factor precursor
108. Cloning and expression of a Saccharomyces diastaticus Glucoamylase gene in Saccharomyces cerevisiae and Schizosaccharomyces pombe
109. Induced expression using yeast copper metallothionein promoter
110. Expression of the α‐galactosi‐dase from Cyamopsis tetragonoloba (guar) by Hansenula polymorpha
111. Genes requried for completion of import of proteins into the endoplasmic reticulum in yeast
112. Regulation of glucose metabolism in growing yeast cell.s
113. Controlled expression and purification of human immune interferon from high‐cell‐density fermentations of Saccharomyces cerevisiae
114. Stable multicopy vectors for high‐level secretion of recombinant human serum albumin by Kluyveromyces yeasts
115. Construction of LYS2 cartridges for use in genetic manipulation of Saccharomyces cerevisiae
116. Tandem gene amplification mediates copper resistance in yeast
117. Scarcity of ARS sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica
118. Expression and secretion of bovine prochymosin in Yarrowia lipolytica
119. Selection and characterisation of ricin toxin A‐chain mutations in Saccharomyces cerevisiae
120. Protein disulphide isomerase: multiple roles in the modification of nascent scretory proteins
121. Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe
122. The 2μm circle plasmid of Saccharomyces cerevisiae
123. Maintenance of the 2μm circle plasmid in populations of Saccharomyces cerevisiae
124. Copy number and stability of 2‐μm circle‐based artificial plasmids of Saccharomyces cerevisiae
125. Efficient secretion of human parathyroid hormone by Saccharomyces cerevisiae
126. Replication and recombination of 2μ DNA in Schizosaccharomyces pombe
127. LEU2‐directed expression of β‐galactosidase activityand phleomycin resistance in Yarrowia lipolytica
128. Intefrative transformation of the yeast Yarrowia lipolytica
129. Large and small invertase and the yeast cell cycle: pattern of synthesis and sensitivity ot tunicamycin
130. Heterologous gene expression in Hansenula polymorpha: Efficient secretion of glucoamylease
131. Characterisaton of recombinant human epidermal growth factor produced in yeast
132. High frequency of yeast transformation by plasmids currying part or entire 2‐μm yeast plasmid
133. Protein folding in the cell
134. A novel screening system for yeast strains capable of secreting tissue plasminogen activator
135. Molecular regulation of methanol oxidase activity in continuous cultures of Hansenula polymorpha
136. Transformation of the methylotrophic yeast Hansenula polymorpha
137. The methylotrophic yeasts
138. Cauliflower mozaic virus promoters direct the efficient expression of a bacterial G418 resistance gene in Schizosaccharomyces pombe
139. The expression of calf prochymosin in Saccharomyces cerevisiae
140. The selection of promoters for the expression of heterologous genes in hte yeast Saccharomyces cerevisiae
141. Secreetion of somatostatin by Saccharomyces cerevisiae. Correct processing of an α‐factor‐somatostatin hybrid
142. Size distribution and general structural features of N‐linked oligosaccharides from the methylotrophic yeast, Pichia pastoris
143. Plasmid encoded hygromycin B resistance: the sequence of hygromycin B phosphotronsferase and its expression in Escherichia coli and Saccharomyces cerevisiae
144. Growth control in fed‐batch cultures of recombinant Saccharomyces cerevisiae producing hepatitis B surface antigen (HBsAg)
145. Production and purificaton of recombinant human interleukin‐6 secreted by the yeast Saccharomyces cerevisiae
146. An efficient chloramphenicol‐resistance marker for Saccharomyces cerevisiae and Escherichia coli
147. G418‐resistance as a cloning marker and reporter for gene expression in Saccharomyces cerevisiae
148. Expression of streptokinase in Pichia pastoris yeast
149. A deletion that includes the signal peptidase cleavage site impairs processing, glycosylation and secretion of cell surface yeast acid phosphatase
150. Amino terminal acetylation of authentic human Cu, Zu superoxide dismutase produced in yeast
151. Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae
152. The transformation of brewing yeasts with a plasmid containing the gene for copper resistance
153. Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae
154. Sequence of a Drosophila DNA segment that functions in Saccharomyces cerevisiae and its regulation by a yeast promoter
155. Identification and comparison of stable and unstalbe mRNAs in Saccharomyces cerevisiae
156. Genetics and genetic engineering of the industrial yeast Yarrawia lipolytica,
157. Cloning of the alkaline extracellular protease gene of Yarrowia lipolytica and its use to express foreign genes
158. Replicating plasmids in Schizosaccharomyces pombe: Improvement of symmetric segregation by a new genetic element
159. Genetic transfer of the pigment bacteriorhodopsin into the eukaryote Schizosaccharomyces pombe
160. DMSO‐enhanced whole‐cell yeast transformation
161. Transformation of yeast
162. Heterologous gene expression in yeast
163. Yeast Saccharomyces cerevisiae proteinases: structure, characterisitics and function
164. Use of heterologous and homologous signal sequences for secretion of heterrologous proteins from yeast
165. Expression of human gene for interferon in yeast
166. Secretion of human interferons by yeast
167. Secretion of human serum albumin form Hansenula polymorpha
168. Codon replacement in the PGKI gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in goen expression
169. A transcriptionally regulated expression vector for the fission yeast Schizosaccharomyces pombe
170. Structural comparsion of two nontandemly repeated yeast glyceraldehyde‐3‐phosphate dehydrogenase genes
171. Cloning with 2‐μm DNA vectors and the expression of foreign genes in Saccharomyces cerevisiae
172. Transfromation of Schizosaccharomyces pombe by electroproation
173. Secretion of functional antibody and Fab fragment from yeast cells
174. Galactose as a gratuitous inducer of GAL gene expression in yeasts growing on glucose
175. Controlled fed‐batch fermentation of recombinant Saccharomyces cerevisiae to produce hepatitis B surface antigen
176. Specificity of cotranslational amino‐terminal processing of processing of proteins in yeast
177. Homologous mRNA 3′ end formation in fission and budding yeast
178. The effects of culture conditions on the secretion of human lysozyme by Saccharomyces cerevisiae A2–1‐1A
179. Expression, glycosylation and secretion of an Aspergillus glucoamylase by Saccharomyces cerevisiae
180. Transcription of the ADH2 gene in Saccharomyces cerevisiae is limited by positive factors that bind competitvely to its intact promotert region on multicopy plamids
181. Different classes of polyadenylation sites in the yeast Saccharomyces cerevisiae
182. Transformation of intact yeast cells treated with alkali cations
183. Synthesis in yeast of hepatitis B virus surface antigen modified P31 particles by hene modification
184. Influenza viral (A/WSN/33) hemagglutinin is expressed and glycosylated in the yeast Saccharomyces cerevisiae
185. Expression of human liver epoxide hydrolase in Schizosaccharomyces pombe
186. Simultaneous synthesis and assembly of various hepatitis B surface protein in Saccharomyces cerevisiae
187. Expression and characterisation of infectious bursal disease virus polyprotien in yeast
188. Fatty acylated proteins as componets of intracelluar signaling pathways
189. Synthesis of Escherichia coli outer membrane protein OmpA in yeasts
190. Simultaneous expression of the S and L surface antigens of gepatitis B, and formation of mixed particles in the methylotrophic yeast, Hansenula plymorpha
191. Expression system based on the methylotrophic yeast Hansenula polymorpha
192. Expression of the four subunits of the Torpedo californica nicotinic acetlycholine receptor in Saccharomyces cerevistae
193. Expression of a transposable antibiotic resistance element in Saccharmoyces
194. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiar
195. Interaction of postive and negative regulatory proteins in the galactose‐regulon of yeast
196. Expression of the SV40 promoter in fission yeast: indentification and characterisation of an API‐like factor
197. Yeast α‐factor is processed from a larger precursor ploypeptide: The essential role of a mambrane‐bound dipeptidyl aminopeptidase
198. Many random sequences functionally replace the secretion signal of yeast invertase
199. In vivo construction of linear vectors based on killer plasmids from Khiyyeromyces lactis: Selection of a nuclear gene resluts in attachment of telomeres
200. New recombinant linear DNA elements derived from Kluyveromyces lactis killer plasmids
201. Cotranslational amino‐terminal processing
202. Protein aggregation in vitro and in vivo: a quantitative model of the kinetic competition between folding and aggregation
203. Yeast plasmid requries a cisacting locus and two plasmid proteins for its stable maintenance
204. The primary sturcture of the leul gene of Schizosaccharomyces pmobe
205. The production of proteins and peptides from Saccharomyces cerevisiae.
206. sα subunit
207. High‐effciency yeast expression vectors based on the promoter of the phoshoglycerate kinase gene
208. Heterolohous gene expression in Saccharomyces cerevisiae
209. The Herpes simplex virus thymidine kinase is not transcirbed in Saccharomyces cerevisiac
210. Unusually high‐level expression of a foreing gene (hepatitis B virus core antigen) in Saccharamoyces cerevisiac
211. Control of formation of active soluble or inactive insoluble baker's yeast alpha‐glucosidase P1 in Escherichia coli by induction and growth temperature
212. Evaluation of foreign gene codon optimisation in yeast: expression of a mouse Ig kappa chain
213. Assembly of asparagine‐linked oligosaccharies
214. The scanning model for translation: an update
215. Regulated expression of a human interferon gene in yeast: control by phoshpate concentration or temperature
216. Consturction of an expression vector for the fission yeast Schizosaccharomyces pobmbe
217. Protein glycosylation in yeast
218. Cloning and heterolohous exprission of glycosidase genes form Saccharmoyces cerevisiac
219. Strategies for efficiency and accuracy in gene expression 1. The major codon preference: a growth optimization strategy
220. Transcription from maize storage protein gene promoters in yeast
221. Molecular cloning and characterisation of a gene coding for methanol oxidase an Hansenula ploymarpha
222. Complementation used to clone a human homolohue of the fission yeast cell cycle control gene cdc 2.
223. Expression of active human uterine tissue plasminogen activator in yeast
224. The upstream activation site of a Ty2 element of yeast is necessary but not sufficient to promote maximal transcription of the element
225. Secretion of N‐glycosylated human recombinant interleukin‐1β in Saccharomyces cerevisiae
226. Secretion of N‐glycosylated interleukin‐1β in Saccharomyces cerevisiac using a leader peptide from Candida albicans. Effact of N‐linked glycosylation on biological activity
227. The C terminus of mouse ornithine decarboxylase confers rapid degradation on dihydrofolate reductase
228. Plasmid‐transformed URA3FURI double‐mutants of S. cerevisiae: an autoselection system applicable to the production of foreign proteins
229. High level of expression of a protective antigen of schistosomes in Saccharomyces cerevisiae
230. Mechanism of high‐copy‐number intergration of pMIRY‐type vectors into the rebosomal DNA of Saccharmoyces cerevisiae
231. High‐copy‐number integration into the ribosomal DNA of Saccharmoyces cerevisiac: a new vector for high‐level expression
232. Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcriptional regulation in a foreign environemnt
233. MRNA stability in S. cerevisiac
234. High level heterologous expression in E. coli using mutant forms of the lac promoter
235. Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons
236. Protective surface antigen P69 of Bordetella pertussis: its characterisation and very high level expression in Escherichia coli
237. The RAD52 gene is requied for homothallic interconversion of mating types and sopnatneous mitotic recombination in yeast
238. A transcription map of a yeast centromere plasmid: unexpected transcripts and altered gene expression
239. The purification of eukaryotic polypepties synthesised in Escherichia coli
240. Intracelluar precursors and secretion of the alkaline exreacellular protease of Yarrowia Lipolytica
241. Recessive mutations conferring resistance to carbon catabolite repression of galactokinase sysnthesis in Saccharomyces cerevisiae
242. nmtl of fission yeast
243. Human hepatitis B vaccine form recombinant yeast
244. The yeast 2μ plasmid: strategies for the survival of a selfish DNA
245. High efficiency transformation of intact yeast cells by electroporation
246. A transcriptional activator is located in the coding region of the yeast PGK gene
247. Factors affecting heterologous gene expression in Saccharomyces cerevisiae
248. Characterisation of a nonglycosylated single chain urinary plasminogen activator secreted from yeast
249. Expression of plasmid R388 encoded type II dihydrofolate reductase as a dominant selectable marker in Saccharmoyces cerevisiae
250. Expression of murine and human GM‐CSF factors in S. cerevisiae: mutagenesis of the potential glycosylation sites
251. Molecular cloning and regulated expression of the human c‐myc gene in Escherichia coli and Saccharomyces cerevisiae: comparsion of the protein products
252. Secretion of an active nonglycosylated form of the repressible acid phosphastasae of Saccharomyces cerevisiac in the presence of tunicamycia at low temperaures
253. The specificites of yeast nethgioning aminopeptidase and acetylation of amino‐terminal methionine in vivo
254. Production of proteins by secretion form yeast
255. Glycosylation and secretion of human α‐1‐antitrypsin by yeast
256. Subcellular localisation and glycoprotein nature of the invertase form the fission yeast Schizosaccharomyces pombe
257. Pedigree analysis of plasmid segregation in yeast
258. Bending the rules: the 2μ plasmid of yeast
259. Functional analysis of the yeast plasmid partition locus STB
260. Cassette mutagenic analysis of the yeast invertase signal peptide: Effects on protein translocation
261. Changes in a mammalian signal sequence requried for efficient protien secretion by yeast
262. Cloning, sequencing and amplofication of the alkaline exreacxellular protease gene of the yeast Yarrowia lipolytica
263. Expression of invertase activity in Yarrowia lipolytica and its use as a selective marker
264. Use of ARS18‐based vectors to increase protein production in Yarrowia lipolytica
265. S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP
266. Development of genetic maps of non‐conventional yeasts
267. Development of genetic techniques and the genetic map of the yeast Saccharamycopsis lipolytica
268. Regulation of extracellular protease production in Candida lopolytica
269. Genetics of exracellular protease production in Saccharomycopsis lipolytica
270. Multiple, tandem plasmid integration in Saccharomyces cerevisiae
271. Genetic application of yeast transformations with linear and gapped plasmids
272. Mutational analysis of a yeast transcriptional terminator
273. N‐Glycosytlation and in vivo enzymatic activity of recombinant human tissue plaminogen activator expressed in Chinese hamster ovary cells and a murine cell line
274. Vector systems for the expression, analysis and cloning of DNA sequences in Saccharoyces cerevistae
275. Escherichia coli ribosomes translate in vivo with variable rate
276. Effect of ochre nonsense mutations on yeast URA1 mRNA stability
277. Effcient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae,
278. Expression of disptheria toxin fragment A and hormone‐toxin fusion proteins in toxin‐resistant yeast mutants
279. Presecretory and cytoplasmic invertase polypeptides encoded by distince mRNAs derived form the same structural gene differ by a signal sequence
280. An inducible expression vector for both fission and budding yeast
281. Recombinant HIV1 protease secreted by Saccharmoyces cerevisiae. correctly processes myristylated gag polyprotein
282. Expression of the β‐glucuronidase gene under the control of the Ca MV 35S promoter in Schizosaccharomyces pombe
283. Expression. purification and characterisation of recombinant murine granulocyte‐macrophage colony‐stimulation gactor and bovine interleukin‐2 from yeast
284. Expression of heterologous protins in Saccharomyces cerevisiae using ADH2 promoter
285. Translation and stability of an Escherichia coli β‐galactosidase mRNA expressed under the control of pyruvate kinase sequences in Saccharomyces cerevisiae
286. An androgen‐inducible expression system for Saccharmomyces cerevisiae
287. Production of recombinant human serum albumin form Saccharomyces cerevisiae
288. Glycobiology
289. Effect of the deletion of the MFα‐factor pro‐regine on the expression and secretion of human parathyroid hormone in Saccharmoyces cerevisiae
290. Characterisation of a K26Q site‐directed mutant of humarn prarthyroid hormone expressed in yeast
291. Effects of glucose on the activity and synthesis of fermentative and respiratory pathways of Saccharomyces sp
292. Removal of N‐glycosylation sites of the yeast acid phosphatase serverly affects protein folding
293. Characterisation of recombinant factor XIIIa produced in Saccharmoyces cerevisiae
294. Amino acid sequence common to rapidly degraded proteins: the PEST gypothesis
295. Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors
296. Experssion and processing of bacterial β‐lactamase in the yeast Saccharomyces cerevisiae
297. Direct selection of stabilised yeast URA3 transformants with 5‐fluorouracil
298. A transcriptional barrier to expression of colned toxin genes of the linear plasmid k1 of Khuyveromyces lactis: evidence that native K1 has novel promoters
299. Recombinant Bordetella pertussis pertacin (P69) form the yeast Pichia pastoris: high‐levle production and immunological properties
300. Expression of tetanus toxin fragment C in yeast: gene synthesis is required to eliminate fortuiltous polyadenylation sites in AT‐rich DNA
301. Sturcture and functiono of the yeast URA3 Gene: expression in Escherichia coli
302. KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene
303. 1‐antitrypsin
304. Glyceraldehyde‐3 phosphate dehydrogenase‐derived expression cassettes for constitutive synthesis of heterologous proteins
305. Secretion in yeast: structural features influencing the post‐translational translocation of prepro‐α‐factor in vitro
306. One‐step gene disruption in yeast
307. Synthesis and secretion of wheat α‐amylase in Saccharomyces cerevisiae
308. 2+ ATPase family
309. Efficient secretion of Bacillus amylolique faciens α‐amylase by its own signal peptide from Saccharomyces crevisiae host cells
310. Evolutionary divergence of the mRNA transcription initiation mechanism in yeast
311. Transcription of the triosephosphate isomerase gene of Schizosaccharomyces pombe initiates from a start point different from that in Saccharomyces cerevisiae
312. Gene cloning and expression in fission yeast
313. The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe
314. HIgh‐level expression and in vivo processing of chimeric ubiquitin fusion proteins in Saccharomyces cerevisiae
315. Enhanced secretion of human nerve growth factor from Saccharomyces cerevisiae using an advanced δ‐integration system
316. High frequencey contransformation by copolymerisation of plasmids in the fission yeast Schizosaccharomyces pombe
317. The alcohol dehydrogenase system in the yeast, Kluyyeromyces lactis
318. The fos gene product undergoes extensive post‐translational modification in eukaryotic but not in prokaryotic cells
319. Properties of promotes cloned randomly from the Saccharomyces crevisiae genome
320. Messenger RNA stability in Saccharomyces cerevisiae: The influece of translation and poly(A) tail lenght
321. The conformation of mature α‐amylase conditions its secretion from yeast
322. Primary structure, genomic organisation and heterologous expression of a glucose tranporter from Arbidopsis thaliana
323. Enzymantic coupling of cholesterol intermediates to a mating pheromone precusor and to the ras protein
324. Formation of soluble recombinant proteins in Escherichia coli is favored by lower growth tempeature
325. Vectors for constituve and inducible gene expression in yeast
326. Targeting and assemble of and oligomeric bacterial entertoxoid in the endoplasmi reticulum of Saccharomyces crevisiae
327. Regulated overproduction of the GAL4 gene product greatly increases expression from galactose‐inducible promoters on multi‐copy expression vectors in yeast
328. Expression and secretion in yeast of a 400‐kDa envelope glycoprotein derived from Epstein‐Barr virus
329. Amino acid misicorporation during high‐level expression of mouse epidermal growth factory in Escherichia coli
330. Premilinary studies on a citric acid producing strain of Saccharomycopsis lipolytica
331. Synonymous codon usage in Saccharomyces cerevisiae
332. A conserved AU sequence from the 3′ unstranslated region of GMCSF mRNA mediates selective mRNA degradation
333. Synthesis and secretion of hapatitis middle surface antigen by the methylotrophic yeast Hansenula polymorpha
334. Protein fusions: bioseparation and application
335. Construction and genetic characterisation of temperature‐sensitive mutant alleles of the yeast actin gene
336. Regulated transcriptional systems for the production of proteins in yeast: regulation by carbon source
337. Kluyveromyces lactis glyceraldehyde‐3‐phosphate dehydrogenase and alcohol dehydrogenase‐1 genes are linked and divergently transcribed
338. Integration and amplification of DNA at yeast delat sequences
339. Sequence of the Kluyveromyces lactis URA3 gen
340. Yeast mutants conferring resistance to toxic effects of clined human insulin‐like growth factor I
341. Optimisation of a host/vectors system for heterologous gene expression by Hansenula polymorpha
342. The yeast acid phosphatase can enter the secretory pathway without its N‐terminal signal sequencs
343. Synthesis, secretion and procesing of α‐factor‐interferon fusion proteins in yeast
344. Use of the Glu‐Glu‐Phe C‐terminal epitode for rapid purification of the catalytic domain of normal and mutant ras GTPase‐activating proteins
345. Construction of temprerat ure‐regulated yeast promoters using the MATα2 repression system
346. Superimposition of temperature regulation on yeast promoters
347. Saccharomyces crevisiae strains that overexpress heterologouls protiens
348. The secretion of human serum albumin from the yeas Saccharomyces cerevisiae using five different leader sequences
349. Cloning characterisation of the Saccharomyces cerevisiae glycerol‐3‐phosphate dehydrogenase (GUT2) promoter
350. Heterologous protein secretion from yeast
351. Expressin of cDNAs encoding barley α‐amylase 1 and 2 in yeast and characterisation of the secreted proteins
352. High‐level expression, purification, and characterisation of recombinant human tumour necrosis factor synthesised in the methylotrophic yeast Pichia pastoris
353. High level expressionof heterologous proteins in methylotrophic yeast Pichia pastrois
354. Invertase gene (SUC2) of Saccharomyces cerevisiae as dominant market for transformation of Pichia pastoris
355. Transformation of Kluyvermyces lactis with the kanamycin (G418) resistance gene of Tn
356. The organisation and transcription of the galactose gene cluster of Saccharomyces
357. The plasmid‐encoded killer system of Kluyveromyces lactis: a review
358. Cloning and analysis of the Kluyvermyces lactis TRP1 gene: a chromosomal locust flanked by genes encoding inorganic phrophoshatase and histone H3
359. Synthesis of a human insulin gene VI. Expression of the synthetic proinsulin gene in yeast
360. α‐factorleader‐directed secretion of rembinant humaninsulin‐like growth factor I from Saccharomyces cerevisiae
361. Analysis of the α‐amylase gene of Schwanniomyces ocidentails and the secretion of its gene product in transformants of different yeast genera
362. Molecular mechanism of transcriptional regulation in yeast
363. A physical, genetic of Saccharomyces cerevisiae
364. Signals for transcription initiation and termination in teh Saccharomyces cerevisiae plasmid 2μm circle
365. Degradation of yeast recombinant desulfato‐hirudin variant 1 and its mutants by the two carboxypeptidases yscY and yscα
366. Effect of glycosylation on yeast invertase oligomer stability
367. r gene in linear killer DNA plasmids in yeast
368. Secrection and processing of insulin precursors in yeast
369. Producion and some enzymatic properties of alkaline proteinase of Candiada lipolytica
370. A process for the production of human proinsulin in Saccharomyces cerevisiae
371. Human chorionic gonadotropin and human cytomegalovirus promoters are extremely active in the fissin yeast Schizosaccharomyces pombe
372. Structrue of oligosaccharides on Saccharomyces SUCZ invertase secreted by the methylotrophic yeast Pichia pastoris
373. Expression of the lacZ gen from two methanol‐regulated promoters in Pichia pastoris
374. High‐level secretion of glycosylated invertase in the methylotrophic yeast,Pichia pastoris
375. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene
376. Regulated high efficience expression of human interferon‐alpha in Saccharomyces cerevisiae
377. Optimisation of prourokinase secretion froma recombinant Saccharomyces cerevisiae
378. Chemical synthesis oa gene for huma epidermal growth facror urogastron and its expression in yeast
379. Properties and distribution of soybean proglycinin expressed in Saccharomyces cerevisiae
380. Synthesis and assembly of hepatitis B virust surgace antigen particles in yeast
381. Cloning, characterisation and expression in Saccharomyces crevisiae of endoglucanase I from Trichoderma reesei
382. Effect of deletions in the 5′‐noncoding region on the translational efficiency of phosphoglycerate kinase mRNA in yeast
383. Effect of leader primary structure on the translational efficiency of phosphoglycerate kinase mRNA in yeast
384. Kluyveromyces as a host for heterologous gene expression: Expression and secretion of prochymosin
385. Signal peptides I overproduction results in increased efficienceis of exportand maturation of hybrid secretory proteins in Escherichia coli
386. Targeting of proteins for degradation
387. The yeast phosphatase system
388. Deoxyribonucleic acid plasmids in yeasts
389. Synthesis of wild type and mutant hemoglobins in Saccharomyces crevisiae
390. Stability of a cloned gene in yeast grown in chemostat culture
391. Negative regulation of gene expression by matting type
392. Multiple chemical forms of hepatitis B surface antigen produced in yeast
393. Direct selection of Saccharomyces cerevisiae resistant to the antibiotic G418 following transformation with a DNA vector carrying the kananmycin resistance gene of Tn903
394. Sexual reproduction in Candida lipolytica
395. HIV expression strategies: ribosomal frameshifting is directed by a short sequence in both mammalian and yeast systems
396. Development of the genetics of the dimorphic yeast Yarrowia Lipolytica
397. The synthesis and in vivo assembly of functional antibodies in yeast
398. Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Yarrowia lipolytica by targeted integration
399. Yeast signal peptidase contains a glycoprotein and the SecII gene product
400. Extruacullular acid proteases produced by Sacchoaromycopsis lipolytica
401. Fission yeast
402. Secretion of Mucor rennn, a fungal aspartic protease of Mucor pusillus by recombinant yeast cells
403. Expression of Torpedo nicotinic acetylcholine receptor subunits in yeast is enhanced by the use of yeast signal sequences
404. Use of lacZ fusions to delimit regulatory elements of the inducible GAL1‐GAL10 promoter in Saccharomyces cerevisiae
405. A 3′transcriptional enhancer within the coding sequence of a yeast gene encoding the common suunit of two multienzyme complexes
406. DNA sequence required for efficient transcription termination in yeast
407. Mutationally altered 3′ ends of yeast CYC1 mRNA affect transcript stability and translational efficiency
408. Production and secretion of porcine urokinase in Saccharomyces cerevisiae: characterisation of the secreted gen product
409. Amplification of plasmid copy number by thymidine kinase expression in Saccharomyces cerevisiae
410. Control of Herpes simplex virus thymidine kinase gene expression in Saccharomyces cerevisiae by a yeast promoter sequence
411. Protein secretion from Saccharomyces cerevisiae directed by teh prepro‐α‐factor region