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Volumn 256, Issue 5065, 1992, Pages 1808-1812

Cytoplasmic domain heterogeneity and functions of IgG Fc receptors in B lymphocytes

Author keywords

[No Author keywords available]

Indexed keywords

FC RECEPTOR; IMMUNOGLOBULIN G;

EID: 0026769620     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1535455     Document Type: Article
Times cited : (404)

References (38)
  • 26
    • 0021895138 scopus 로고
    • Intracellular Ca2+ concentrations were measured in single cells that had been exposed to fura-2 and analyzed by phocal microfluorometry (Joyce-Loeble, Cambridge, England) as described (by Cells were incubated with 1 μM fura-2-acetoxy-methyl-ester (Molecular Probes) for 15 min at 37°C and then plated in culture medium on a polylysine-coated (500 μg/ml, 10 min) glass cover slip. The fluorescence on single cells was measured. Absolute intracellular calcium concentrations were calculated online by the Phocal software, on an IBM AT computer. Anti-IgG (±2.4G2 Fab) was diluted in Ringer solution at the indicated concentrations (Fig. 4) and applied by pressure ejection through a glass pipette
    • (1985) J. Biol, Chem , vol.260 , pp. 3440
    • Grynkiewicz, G.1    Poenie, M.2    Tsien, R.Y.3
  • 32
    • 0023646810 scopus 로고
    • Cells resistant to G418 (1 mg/mI) were cloned in soft agar, and several individual cell lines, expressing similar numbers of wild-type or mutant FcγRll were selected
    • (1987) Cell , vol.51 , pp. 503
    • Thomas, K.R.1    Capecchi, M.R.2
  • 34
    • 0018291288 scopus 로고
    • Expression was quantified by determining the binding of 125II-labeled Fab fragments of the FcγRII-specific monoclonal antibody 2.4G2 All cells (including the A20 parent cell line) expresses between 1 x 105 to 4 x 105 receptors per cell.
    • (1979) J. Exp. Med , vol.150 , pp. 580
    • Unkeless, J.C.1
  • 35
    • 0023571895 scopus 로고
    • Culturing of the 24.4 T cell hybridomas and antigen presentation assays were performed as described The IC's were formed prior to use by incubating different concentrations of purified X repressor with (at 15 μ/ml) each of two different monoclonal antibodies to λ repressor (51F and 22D) at 37°C for 15 min. Release of IL-2 was determined by monitoring the growth of the IL-2-dependent cell line CTL.L2 as described (33)
    • (1987) J. Immunol , vol.139 , pp. 3973
    • Lai, M.-Z.1
  • 37
    • 85027744664 scopus 로고    scopus 로고
    • it was provided by Institut Cochin de Genetique Moleculaire, Paris). The monoclonal antibodies to λ repressor were purified from culture supernatants by chromatography (MAB-trap G-protein columns; Pharmacia, Bois d'Arcy, France). The hybridoma T cell 24.4, was isolated from BALB/c mice injected with the 12–24 peptide of λ-repressor as described (33
    • Breyer, R.M.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.