Structural analysis of ternary complexes of Escherichia coli RNA polymerase. Deoxyribonuclease I footprinting of defined complexes

Journal of Molecular Biology

Volumn 225, Issue 2, 1992, Pages 239-250

  Krummel, Barbara ^a   Chamberlin, Michael J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

E. coli; elongation; RNA polymerase; transcription

Indexed keywords

ESCHERICHIA COLI;

RNA; RNA POLYMERASE;

ARTICLE; DNA FLANKING REGION; DNA FOOTPRINTING; DNA PROTEIN COMPLEX; DNA SEQUENCE; DNA STRAND; DNA TEMPLATE; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; TRANSCRIPTION TERMINATION;

BASE SEQUENCE; DEOXYRIBONUCLEASE I; DNA, BACTERIAL; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MACROMOLECULAR SYSTEMS; MOLECULAR SEQUENCE DATA; RNA, MESSENGER; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC;

EID: 0026766857     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-2836(92)90918-A     Document Type: Article

References (26)

1. RNA chain elongation by Escherichia coli RNA polymerase Factors affecting the stability of elongating ternary complexes
2. RNA 3′ end formation in the control of gene expression
3. A topological model for transcription based on unwinding angle analysis of E. coli RNA polymerase binary, initiation and ternary complexes
4. Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site
5. DNA-dependent RNA polymerase of Escherichia coli induces bending or an increased flexibility of DNA by specific complex formation
6. A general method of in vitro prepraration and specific mutagenesis of DNA fragments study of protein DNA interactions
7. On the road with RNA polymerase; regulation of transcript elongation and termination
8. Structural transitions of E. coli RNA polymerase during transcription initiation and elongation
9. RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes
10. Structural analysis of ternary complexes of Escherichia coli RNA polymerase. Individual complexes halted along different transcription units have distinct and unexpected biochemical properties
11. Mapping and characterization of transcriptional pause sites in the early genetic region of bacteriophage T7
12. Isolation and properties of transcribing ternary complexes of Escherichia coli RNA polymerase positioned at a single template base
13. RNA polymerase II elongation complexes paused after the synthesis of 15- or 35-base transcripts have different structures
14. A new method for sequencing DNA
15. A cinematographic view of Escherichia coli RNA polymerase translocation
16. Footprinting analysis of mammalian RNA polymerase II along its transcript: an alternative view of transcription elongation
17. Phage lambda and the regulation of transcription termination
18. Transcription elongation and eukaryotic gene regulation
19. Genetic and physical mapping of the late region of bacteriophage T7 DNA by use of cloned fragments of T7 DNA
20. Spontaneous cleavage of RNA in ternary complexes of E. coli RNA polymerase, and its significance for the mechanism of transcription
21. Terminator-distal sequences determine the in vitro efficiency of the early terminators of bacteriophages T3 and T7
22. Protein-nucleic acid interactions in transcription a molecular analysis
23. Transcript elongation and termination in Escherichia coli
24. Specificity and mechanism of antitermination by Q proteins of bacteriophages λ and 82
25. Transcription attenuation