Topoisomerases and yeast rRNA transcription: Negative supercoiling stimulates initiation and topoisomerase activity is required for elongation

Genes and Development

Volumn 6, Issue 7, 1992, Pages 1332-1341

  Schultz, Michael C ^a   Brill, Steven J ^b,d   Ju, Qida ^c   Sternglanz, Rolf ^b   Reeder, Ronald H ^a  

^a FRED HUTCHINSON CANCER RESEARCH CENTER   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^d Simons Center for Quantitative Biology   (United States)

Author keywords

RNA polymerase I; Supercoiling; Topoisomerases; Transcription; Yeast

Indexed keywords

DNA DIRECTED RNA POLYMERASE; DNA TOPOISOMERASE; RIBOSOME DNA; RIBOSOME RNA;

ARTICLE; DNA TRANSCRIPTION; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME LOCALIZATION; ENZYME MECHANISM; NONHUMAN; PLASMID; PRIORITY JOURNAL; RNA SYNTHESIS; RNA TRANSCRIPTION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION INITIATION;

CLONING, MOLECULAR; DNA TOPOISOMERASES, TYPE I; DNA, FUNGAL; DNA, SUPERHELICAL; MODELS, GENETIC; RESTRICTION MAPPING; RNA POLYMERASE I; RNA, FUNGAL; RNA, RIBOSOMAL; SACCHAROMYCES CEREVISIAE; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC;

EID: 0026725797     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.6.7.1332     Document Type: Article

References (21)

1. Brill, S.J. and R. Sternglanz. 1988. Transcription-dependent DNA supercoiling in yeast DNA topoisomerase mutants. Cell 54: 403-411.
2. Brill, S.J., S. DiNardo, K. Voelkel-Meiman, and R. Sternglanz. 1987. Need for DNA topoisomerase activity as a swivel for DNA replication and for transcription of ribosomal RNA. Nature 326: 414-416.
3. Choe, S.Y., M.C. Schultz, and R.H. Reeder. 1992. In vitro definition of the yeast RNA polymerase I promoter. Nucleic Acids Res. 20: 279-285.
4. Culotta, V. and B. Sollner-Webb. 1988. Sites of topoisomerase I action on X. laevis ribosomal chromatin: Transcriptionally active rDNA has an ∼200 bp repeating structure. Cell 52: 585-597.
5. Dunaway, M. 1990. Inhibition of topoisomerase II does not inhibit transcription of RNA polymerase I and II genes. Mol. Cell. Biol. 10: 2893-2900.
6. Earnshaw, W.C., B. Halligan, C.A. Cooke, M.M.S. Heck, and L.F. Liu. 1985. Topoisomerase II is a structural component of mitotic chromosome scaffolds. J. Cell Biol. 100: 1706-1715.
7. Elion, E.A. and J.R. Warner. 1984. The major promoter element of rRNA transcription in yeast lies 2 kb upstream. Cell 39: 663-673.
8. Fleischman, G., G. Pflugfelder, E. Steiner, K. Javaherian, G. Howard, J. Wang, and S. Elgin. 1984. Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome. Proc. Natl. Acad. Sci. 81: 6958-6962.
9. Giaever, G.N. and J.C. Wang. 1988. Supercoiling of intracellular DNA can occur in eukaryotic cells. Cell 55: 849-856.
10. Higashinakagawa, T., H. Wahn, and R.H. Reeder. 1977. Isolation of ribosomal gene chromatin. Dev. Biol. 55: 375-386.
11. Klootwijk, J. and R.J. Planta. 1989. Isolation and characterization of yeast ribosomal RNA precursors and preribosomes. Methods Enzymol. 180: 96-109.
12. Liu, L.F. and J.C. Wang. 1987. Supercoiling of the DNA template during transcription. Proc. Natl. Acad. Sci. 84: 7024-7027.
13. Muller, M., W. Pfund, V. Mehta, and D. Trask. 1985. Eukaryotic type I topoisomerase is enriched in the nucleolus and catalytically active on ribosomal DNA. EMBO J. 4: 1237-1243.
14. Rose, M.D., P. Novick, J.H. Thomas, D. Botstein, and G.R. Fink. 1987. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243.
15. Schultz, M.C., S.Y. Choe, and R.H. Reeder. 1991. Specific initiation by RNA polymerase I in a whole-cell extract from yeast. Proc. Natl. Acad. Sci. 88: 1004-1008.
16. Singleton, C.K. and R.D. Wells. 1982. The facile generation of covalently closed, circular DNAs with defined negative superhelical densities. Anal. Biochem. 122: 253-257.
17. Struhl, K., D.T. Stinchcomb, S. Scherer, and R.W. Davis. 1979. High frequency transformation of yeast: Autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci. 76: 1035-1039.
18. Veldman, G.M., R.C. Brand, J. Klootwijk, and R.J. Planta. 1980. Some characteristics of processing sites in ribosomal precursor RNA of yeast. Nucleic Acids Res. 8: 2907-2920.
19. Wu, H.-Y., S. Shyy, J.C. Wang, and L. Liu. 1988. Transcription generates positively and negatively supercoiled domains in the template. Cell 53: 433-440.
20. Yamagishi, M. and M. Nomura. 1988. Deficiency in both type I and type II DNA topoisomerase activities differentially affect rRNA and ribosomal protein synthesis in Schizosaccharomyces pombe. Curr. Genet. 13: 305-314.
21. Zhang, H., J.C. Wang, and L.F. Liu. 1988. Involvement of DNA topoisomerase I in transcription of human ribosomal RNA genes. Proc. Natl. Acad. Sci. 85: 1060-1064.