Site-directed mutagenesis of rat liver NAD(P)H: quinone oxidoreductase: Roles of lysine 76 and cysteine 179

Archives of Biochemistry and Biophysics

Volumn 294, Issue 2, 1992, Pages 434-439

  Ma, Qiang ^a   Cui, Kunyuan ^a   Wang, Regina W ^b   Lu, Anthony Y H ^b   Yang, Chung S ^a  

^a RUTGERS UNIVERSITY   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,6 DICHLOROPHENOLINDOPHENOL; ALANINE; COMPLEMENTARY DNA; CYSTEINE; DICOUMAROL; FLAVINE ADENINE NUCLEOTIDE; LIVER ENZYME; LYSINE; MESSENGER RNA; MUTANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ELECTRON TRANSPORT; ENZYME STABILITY; ESCHERICHIA COLI; IMMUNOBLOTTING; LIVER; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAT; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ANIMAL; BASE SEQUENCE; CLONING, MOLECULAR; COMPARATIVE STUDY; CYSTEINE; CYTOSOL; ENZYME STABILITY; KINETICS; LIVER; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NAD(P)H DEHYDROGENASE (QUINONE); OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION; RATS; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

ESCHERICHIA COLI;

EID: 0026717638     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/0003-9861(92)90708-5     Document Type: Article

References (26)

1. Mutagenicity of quinones: pathways of metabolic activation and detoxification.
2. Expression in Escherichia coli of a Chemically Synthesized Gene for Biologically Active Bovine Acidic Fibroblast Growth Factor
3. Purification and crystallization of rat liver NAD(P)H:(quinone-acceptor) oxidoreductase by cibacron blue affinity chromatography: Identification of a new and potent inhibitor