Selection of phage antibodies by binding affinity. Mimicking affinity maturation

Journal of Molecular Biology

Volumn 226, Issue 3, 1992, Pages 889-896

  Hawkins, Robert E ^a   Russell, Stephen J ^b   Winter, Greg ^a,b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

Author keywords

affinity; antibody; filamentous phage; maturation

Indexed keywords

ANTIBODY; ANTIGEN;

ANTIBODY AFFINITY; ARTICLE; BACTERIOPHAGE; BINDING AFFINITY; DISSOCIATION CONSTANT; GENE MUTATION; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMAL; ANTIBODIES, VIRAL; ANTIBODY AFFINITY; ANTIGEN-ANTIBODY COMPLEX; BACTERIOPHAGES; CHICKENS; CLONING, MOLECULAR; GENE LIBRARY; GENES, IMMUNOGLOBULIN; IMMUNOGLOBULIN FRAGMENTS; KINETICS; MURAMIDASE; MUTAGENESIS; POLYMERASE CHAIN REACTION; SERUM ALBUMIN, BOVINE; SUPPORT, NON-U.S. GOV'T;

FILAMENTOUS BACTERIOPHAGE; MURINAE;

EID: 0026699293     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-2836(92)90639-2     Document Type: Article

References (41)

1. Antibody engineering for the analysis of affinity maturation of an anti-hapten response
2. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site
3. Hormone phage: an enrichment method for variant proteins with altered binding properties
4. Mutation drift and repertoire shift in the maturation of the immune response
5. Small rearrangements in structures of Fv and Fab fragments of antibody D1.3 on antigen binding
6. A surface expression vector for antibody screening
7. Chemical and serological studies with an iodine-containing synthetic immunological determinant 4-hydroxy-3-iodo-5-nitropheriylaeetic acid (NIP) and related compounds
8. A large array of human monoclonal antibodies to type I human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals
9. Making antibody fragments using phage display libraries
10. Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP
11. A simple and efficient procedure for saturation mutagenesis using mixed oligonucleotides
12. High efficiency transformation of E. coli by high voltage electroporation
13. Kinetic maturation of an immune response
14. Antibody framework residues affecting the conformation of the hypervariable loops
15. Studies on the Epstein-Barr virus genome
16. Pore-forming properties of the adsorption protein of filamentous phage fd
17. Adsorption complex of filamentous fd virus
18. Cell selection strategies for making antibodies from variable gene libraries: tapping the memory pool
19. A simple method for site-directed mutagenesis using the polymerase chain reaction
20. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains
21. Generation of a large combinatorial library of the immunoglobulin library in phage lambda
22. Replacing the complementarity-determining regions in a human antibody with those from a mouse
23. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces
24. Step-wise intraclonal maturation of antibody affinity through somatu hypermutation
25. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
26. Selecting high affinity binding proteins by monovalent phage display
27. Phage antibodies: filamentous phage displaying antibody variable domains
28. By-passing immunization: human antibodies from V-gene libraries displayed on bacteriophage
29. By-passing immunisation building high affinity human antibodies by chain shuffling
30. The Croonian lecture, 1989. Antibodies: a paradigm for the biology of molecular recognition
31. Antibody-selectable filamentous fd phage vectors: affinity purification of target genes
32. DNA sequencing with chain-terminating inhibitiors
33. Searching for peptide ligands with an epitope library
34. Analysis of somatic mutation and class switching in naive and memory B cells generating adoptive primary and secondary responses
35. Structural correlates of high antibody affinity: three engineered amino acid substitutions can increase the affinity of an anti-p-azophenylarsonate antibody 200-fold
36. The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique
37. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
38. Variability in the lambda light chain sequences of mouse antibody
39. Man-made antibodies