Interdependence of translation, transcription and mRNA degradation in the lacZ gene

Journal of Molecular Biology

Volumn 226, Issue 3, 1992, Pages 581-596

  Yarchuk, Oleg ^a,b   Jacques, Nathalie ^a   Guillerez, Jean ^a   Dreyfus, Marc ^a  

^a ECOLE NORMALE SUPÉRIEURE   (France)

^b INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

Author keywords

mRNA degradation; RNase E; transcriptional polarity; translation initiation; translation transcription coupling

Indexed keywords

BETA GALACTOSIDASE; MESSENGER RNA; RIBONUCLEASE;

ARTICLE; BINDING SITE; CELL LEVEL; CODON; CONTROLLED STUDY; DEGRADATION; ESCHERICHIA COLI; GENE MUTATION; GENETIC TRANSCRIPTION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; RIBOSOME; RNA ANALYSIS; RNA STRUCTURE; RNA TRANSLATION; TRANSLATION INITIATION;

BASE SEQUENCE; BETA-GALACTOSIDASE; CHROMOSOMES, BACTERIAL; COMPARATIVE STUDY; ESCHERICHIA COLI; GENES, STRUCTURAL, BACTERIAL; GENOTYPE; INTRONS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OLIGODEOXYRIBONUCLEOTIDES; RESTRICTION MAPPING; RIBOSOMES; RNA, MESSENGER; SUPPORT, NON-U.S. GOV'T; TRANSCRIPTION, GENETIC; TRANSLATION, GENETIC;

ESCHERICHIA COLI;

EID: 0026663105     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-2836(92)90617-S     Document Type: Article

References (71)

1. Control of transcription termination
2. Evidence for two functional gal promoters in intact Escherichia coli cells
3. RNase III stimulates the translation of the cIII gene of bacteriophage lambda
4. The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli
5. Mutations that reduce expression from the P2 promoter of the E. coli galactose operon
6. Lack of a 5′ non-coding region in Tn1721 encoded tetR mRNA is associated with a low efficiency of translation and a short half-life in Escherichia coli
7. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding
8. Modulation of chemical composition and other parameters of the cell by growth rate
9. RNA chain growth-rate in E. coli
10. Segment-specific muta genesis of the regulatory region in the E. coli galactose operon: isolation of mutations reducing the initiation of transciption and translation
11. Protein-mediated translational repression
12. E. coli lac operator mRNA affects translation initiation of the β-galactosidase mRNA
13. Evidence that the 5′ end of lac mRNA starts to decay as soon as it is synthesised
14. In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of endogenous proteins
15. Expression of malT the regulator gene of the maltose regulon in E. coli, is limited both at transcription and translation
16. Transcription of single-copy hybrid lacZ genes by T7 RNA polymerase in E. coli
17. Sequence changes preceding a Shine-Dalgarno region influence trpE mRNA translation and decay
18. Genomic sequencing
19. Changes in the half-life of ribosomal protein messenger RNA caused by translational repression
20. pEMBL: a new family of single-stranded plasmids
21. Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12
22. Translational regulation of ribosomal proteins in E. coli: molecular mechanisms
23. What constitutes the signal for the initiation of protein synthesis on E. coli mRNAs'
24. The ompA 5′ untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated in RNA stabilizer whose activity is unrelated to translational efficiency
25. Codon context from weakly expressed genes reduce expression in vivo
26. Purification, structure and properties of hybrid β-galactosidase proteins
27. Post transcriptional regulatory mechanisms in Escherichia coli
28. In the Escherichia coli lacZ gene the spacing between the translating ribosomes is insensitive to the efficiency of translation initiation
29. A role for mRNA secondary structure in the control of translation initiation
30. Translational coupling varying in efficiency between different pairs of genes in the central region of the atp operon of E. coli
31. Growth rate as a variable
32. Processivity errors of gene expression in E coli
33. The instability of messenger RNA in bacteria
34. Transcription and translation initiation frequencies of the Escherichia coli lac operon
35. Kinetics of induced enzyme synthesis: determination of the mean life of galactosidase-specific messenger RNA
36. Processing of RNA transcripts
37. Cleavage of structural proteins during assembly of the head of bacteriophage T4
38. Translational coupling of the two proximal genes in the S10 ribosomal protein operon of E. coli
39. A preliminary map of genomic sites for F attachment in E. coli K12
40. Translational control of procaryotic gene expression
41. Intermediates in the degradation of mRNA from the lac operon of Escherichia coli
42. Genetic studies of cleavage initiated mRNA decay and processing of ribosomal 9S RNA show that the Escherichia coli ams and rne loci are the same
43. The importance of the 5′ region in regulating the stability of sdh mRNA in B. subtilis
44. Electron microscopic visialization of transcription
45. RNase E. an endonuclease, has a general role in the chemical decay of E. coli mRNA: evidence that rne and ams are the same genetic locus
46. LacZ translation initiation mutations
47. Transcription and decay of the lac messenger: Role of an intergenic terminator
48. Culture medium for Enterobacteria
49. Effect of premature termination of translation on mRNA stability depends on the site of ribosome release
50. A conditional lethal mutation in an E. coli strain with a longer chemical lifetime of mRNA
51. The functional stability of the lacZ transcript is sensitive towards sequence alterations immediately downstream of the ribosome binding site
52. Multiple determinants of functional mRNA stability: sequence alterations at either end of the lacZ gene affect the rate of mRNA inactivation
53. Control of functional mRNA stability in bacteria: multiple mechanisms of nucleolytic and non-nucleolytic inactivation
54. Functional inactivation of bacteriophage λ morphogenetic gene mRNA
55. Inhibition of lacZ gene translation initiation in trplac fusion strains
56. Preventing the synthesis of unused transcripts by rho factor
57. Identification and characterization of transcription termination sites in the Escherichia coli lacZ gene
58. Translation initiation controls the relative rates of expression of the bacteriophage lambda late genes
59. Translation and mRNA decay
60. In vitro secondary structure analysis of mRNA from lacZ translation initiation mutants
61. Mutations that affect lamB gene expression at a posttranscriptional level
62. Stability of ribosomal protein mRNA and transnational feedback regulation in E. coli
63. Absolute in vivo translation rates of individual codons in Escherichia coli
64. Autogenous control of E. coli threonyl-tRNA synthetase expression in vivo
65. Inefficient translation initiation causes premature transcription termination in the lacZ gene
66. Polypeptide chain initiation: nucleotide sequences of the three ribosome binding sites in bacteriophage R17 RNA
67. The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene
68. Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts
69. The relation between translation and mRNA degradation in the lacZ gene