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Volumn 257, Issue 5070, 1992, Pages 635-641

Directed evolution of an RNA enzyme

Author keywords

[No Author keywords available]

Indexed keywords

TETRAHYMENA;

EID: 0026649231     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1496376     Document Type: Article
Times cited : (365)

References (75)
  • 39
    • 85045502002 scopus 로고
    • In our study, the PCR was performed under our standard reaction conditions (see legend to Fig. 1), resulting in an error rate of approximately 0.1 percent per position per generation. We have developed a mutagenic PCR procedure that provides an error rate of 0.66 ± 0.13 percent per position (95 percent confidence level)
    • (1992) PCR Methods Appl , vol.2 , pp. 28
    • Cadwell, R.C.1    Joyce, G.F.2
  • 63
    • 0019862344 scopus 로고
    • Individual colonies were chosen at random and grown overnight in liquid media. DNA was prepared by the boiling, lysis method and screened for the insert by restriction digestion
    • (1981) Anal. Biochem , vol.114 , pp. 193
    • Holmes, D.S.1    Quigley, M.2
  • 65
    • 0022353156 scopus 로고
    • with reciprocal primers 5'-GTAAAACGACGGCCAGT-3' and 5'-CATGATTACGAATTCTA-3', which are compatible with the pUC plasmid. Sequencing reactions utilized modified T7 DNA polymerase (Sequenase, USB) and [35S](α-thiosphate) dATP and were analyzed by electrophoresis in a 6 percent polyacrylamide-8 M urea gel. Nucleotide sequences of individual subclones are available on request
    • (1985) Gene Anal. Tech , vol.2 , pp. 89
    • Zagursky, R.1    Baumeister, K.2    Lomax, K.N.3    Berman, M.L.4
  • 75
    • 0007292157 scopus 로고
    • Because of limited product yield, we were forced to work at a substrate concentration [S] ≥ Kmthus Kmshould be regarded as only an estimate of the true value
    • (1952) J. Biol. Chem , vol.199 , pp. 357
    • Hofstee, B.H.J.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.