Temperature-dependent substrate inhibition of the cysteine proteinase (GP57/51) from Trypanosoma cruzi

Molecular and Biochemical Parasitology

Volumn 56, Issue 2, 1992, Pages 335-338

  Lima, Ana Paula C A ^a   Scharfstein, Julio ^a   Storer, Andrew C ^b   Ménard, Robert ^b  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Cysteine proteinase; GP57 51; Substrate inhibition; Temperature; Trypanosoma cruzi

Indexed keywords

CYSTEINE PROTEINASE;

ARTICLE; ENZYME INHIBITION; HOST PARASITE INTERACTION; NONHUMAN; PRIORITY JOURNAL; TRYPANOSOMA CRUZI;

ANIMAL; ANTIGENS, PROTOZOAN; COUMARINS; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEINASE INHIBITORS; DIPEPTIDES; DOSE-RESPONSE RELATIONSHIP, DRUG; GLYCOPROTEINS; HEAT; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; SUPPORT, NON-U.S. GOV'T; TRYPANOSOMA CRUZI;

EID: 0026620531     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(92)90183-K     Document Type: Article

References (14)

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4. Proteinases of Trypanosoma cruzi
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6. Structural and functional identification of GP57/51 antigen of Trypanosoma cruzi as a cysteine proteinase
7. Further characterization of Trypanosoma cruzi GP57/51 as the major antigen expressed by differentiating epimastigotes
8. A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi
9. Inhibitors of the major cysteinyl proteinase (GP57/51) impair host cell invasion and arrest the intracellular development of Trypanosoma cruzi in vitro
10. A model to explain the pH-dependent specificity of cathepsin B-catalysed hydrolyses
11. Engineering of papain: selective alteration of substrate specificity by site-directed mutagenesis
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13. The azocasein-urea-pepstatin assay discriminates between lysosomal proteinases
14. Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi