Notes on a light and electron microscopic double-labeling method combining anterograde tracing with Phaseolus vulgaris leucoagglutinin and retrograde tracing with cholera toxin subunit B

Journal of Neuroscience Methods

Volumn 45, Issue 1-2, 1992, Pages 23-33

  Bruce, Kathy ^a   Grofova, Irena ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Cholera toxin subunit B; Double immunohistochemistry; Electron microscopy; Phaseolus vulgaris leucoagglutinin

Indexed keywords

CHOLERA TOXIN; TRACER; UNCLASSIFIED DRUG; DIAGNOSTIC AGENT; LEUKOAGGLUTININS, PLANTS; PHYTOHEMAGGLUTININ;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; MALE; NERVE PROJECTION; NONHUMAN; PHASEOLUS VULGARIS; PRIORITY JOURNAL; RAT; SPINAL CORD; SUBSTANTIA NIGRA; SYNAPSE; ANIMAL; BRAIN; HISTOLOGY; NERVE FIBER TRANSPORT; NERVE TRACT; RAT STRAIN;

ANIMAL; AXONAL TRANSPORT; BRAIN; CHOLERA TOXIN; MALE; MICROSCOPY, ELECTRON; NEURAL PATHWAYS; PHYTOHEMAGGLUTININS; RATS; RATS, SPRAGUE-DAWLEY; SPINAL CORD; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0026615121     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(92)90040-K     Document Type: Article

References (25)

1. Organization of the thalamostriatal projections in the rat, with special emphasis on the ventral striatum
2. Topographical organization and relationship with ventral striatal compartments of prefrontal corticostriatal projections in the rat
3. PHA-L can be transported anterogradely through fibers of passage
4. Tracing of neuronal connections with cholera toxin subunit B: light and electron microscopic immunohistochemistry using monoclonal antibodies
5. Monoaminergic, peptidergic, and cholinergic afferents to the cat facial nucleus as evidenced by a double immunostaining method with unconjugated cholera toxin as a retrograde tracer
6. Nuclei of origin of monoaminergic, peptidergic, and cholinergic afferents to the cat trigeminal motor nucleus: a double-labeling study with Cholera-toxin as a retrograde tracer
7. An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L)
8. A method for anterograde axonal tracing of chemically specified circuits in the central nervous system: combined Phaseolus vulgaris leucoagglutinin (PHA-L) tract tracing and immunohistochemistry
9. The PHA-L anterograde axonal tracing method
10. Localizing spinal cord-projecting neurons in adult albino rats
11. Monoclonal antibodies to cholera toxin with special reference to cross-reactions with Escherichia coli heat-labile enterotoxin
12. Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons
13. Periodate-lysine-paraformaldehyde fixative a new fixative for immunoelectron microscopy
14. Monosynaptic nigral inputs to the pedunculopontine tegmental nucleus neurons which send their axons to the medial reticular formation in the medulla oblongata. An electron microscopic study in the cat
15. Thyrotropin-releasing hormone-immunoreactive nerve terminals synapse on the dendrites of gastric vagal motoneurons in the rat
16. Labeling of axons of passaged by Phaseolus vulgaris leucoagglutinin (PHA-L)
17. The output neurones and the dopaminergic neurones of the substantia nigra receive a GABA-containing input from the globus pallidus in the rat
18. An approach to tracing neuron networks in the cerebral cortex and basal ganglia. Combination of Golgi staining, retrograde transport of horseradish peroxidase and anterograde degeneration of synaptic boutons in the same material
19. Phaseolus vulgaris leucoagglutinin immunohistochemistry. A comparison between autoradiographic and lectin tracing of neuronal efferents
20. Neural connections identified with PHA-L anterograde and HRP retrograde tract-tracing techniques
21. Organization of the nigrotectospinal pathway in the cat: a light and electron microscopic study
22. The nigrotectal projection and tectospinal neurons in the rat. A light and electron microscopic study demonstrating a monosynaptic nigral input to identified tectospinal neurons
23. Neuroanatomical tracing by use of Phaseolus vulgaris leucoagglutinin (PHA-L): electron microscopy of PHA-L filled neuronal somata, dendrites, axons and axon terminals
24. Double-label immunocytochemistry combination of anterograde neuroanatomical tracing with Phaseolus vulgaris leucoagglutinin and enzyme immunocytochemistry of target neurons