Structure determination and refinement of bovine lens leucine aminopeptidase and its complex with bestatin

Journal of Molecular Biology

Volumn 224, Issue 1, 1992, Pages 113-140

  Burley, Stephen K ^a,b   David, Peter R ^a   Sweet, Robert M ^c   Taylor, Allen ^d   Lipscomb, William N ^a  

^a HARVARD UNIVERSITY   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

^c BROOKHAVEN NATIONAL LABORATORY   (United States)

^d TUFTS UNIVERSITY   (United States)

Author keywords

bestatin; exopeptidase; leucine aminopeptidase; multiple isomorphous replacement; X ray crystallography

Indexed keywords

BOVINAE; ESCHERICHIA COLI; HESPERIA COMMA BOREALIS; BOS TAURUS;

BESTATIN; CYTOSOL AMINOPEPTIDASE; METAL COMPLEX; ZINC;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATTLE; CHEMICAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME INHIBITOR COMPLEX; ENZYME STRUCTURE; ESCHERICHIA COLI; LENS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMAL; BINDING SITES; CATTLE; ENZYME STABILITY; HYDROGEN BONDING; LENS, CRYSTALLINE; LEUCINE; LEUCYL AMINOPEPTIDASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; X-RAY DIFFRACTION;

EID: 0026602711     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-2836(92)90580-D     Document Type: Article

References (98)

1. The Cambridge Crystallographic Data Centre computer-based search retrieval analysis and display of information
2. Kinetic parameters of metal-substituted leucine aminopeptidase from bovine lens
3. Amastatin, an inhibitor of aminopeptidase A, produced by actinomycetes
4. Television area detector diffractometers
5. Processing of the initiation methionine from proteins: Properties of the Escherichia coli methionine aminopeptidase and its gene structure
6. The protein data bank: a computer based archival file for macromolecular structures
7. A system for collection and on-line integration of X-ray diffraction data from a multiwire area detector
8. Analysis of the skeletal configuration of crystalline hen egg-white lysozyme
9. Crystallographic R factor refinement by molecular dynamics
10. Slow-cooling protocols for Crystallographic refinement by simulated annealing
11. Molecular structure of leucine aminopeptidase at 2·7-Å resolution
12. Leucine aminopeptidase: Bestatin inhibition and a model for enzyme-catalyzed peptide hydrolysis
13. Intermolecular cross-linking of monomeric proteins and cross-linking of oligomeric proteins as a probe of quaternary structure
14. 2+ of the zinc metalloenzyme, amino acid composition, and sulfhydryl content
15. Structural aspects of metal ion-carboxylate interactions
16. Interaction of metal ions with carboxylic and carboxamide groups in protein structures
17. Geometry of interaction of metal ions with histidine residues in protein structures
18. Systematics in the interaction of metal ions with the main-chain carbonyl group in protein structures
19. Study of leucinaminopeptidase crystal from bovine pancreas
20. Structural biology of zinc
21. Carboxylate-histidine-zinc interactions in protein structure and function
22. The primary structure of leucine aminopeptidase from bovine eye lens
23. 2+, and of metal-free apoenzyme
24. A method for equilibrating protein crystals with heavy atom reagents
25. Leucine aminopeptidase and other N-terminal exopeptidases
26. Protein, DNA, and virus crystallography with a focused imaging proportional counter
27. The binding center of leucine aminopeptidase. Investigations with substrate analogous and spinlabelled inhibitors
28. An alternative method of solving the layer scaling equations of Hamilton Rollett and Sparks
29. On the importance of orientation in general base catalysis by carboxylate
30. Design of novel inhibitors of aminopeptidases Synthesis of peptide-derived diamino thiols and sulfur replacement analogues of bestatin
31. Characteristics of a flat multiwire area detector for protein crystallography
32. Crystalline leueine aminopeptidase from lens (α-aminoacyl-peptide hydrolase; EC 3.4.11.1)
33. Inhibition of arginine aminopeptidase by bestatin and arphamenine analogues. Evidence for a new mode of binding to aminopeptidases
34. Oscillation method with large unit cells
35. Characterization of metal centres in biological systems by X-ray absorption spectroscopy
36. Representation of phase probability distributions for simplified combination of independent phase information
37. Software for a diffractometer with multiwire area detector
38. Interactive computer graphics: FRODO
39. Using known substructures in protein model building and crystallography
40. Preliminary X-ray study of leucine aminopeptidase (bovine lens). an oligomeric metalloenzyme
41. Evaluation of single-crystal X-ray diffraction data from a position-sensitive detector
42. Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features
43. Atomic structure of the actin: DNase I complex
44. Escherichia coli aspartate transcarbamylase: The relation between structure and function
45. Electron microscopy of leucine aminopeptidase
46. Purification and characterization of human placental microsomal aminopeptidase: Immunological difference between placental microsomal aminopeptidase and pregnancy serum cystylaminopeptidase
47. Rigid protein motion as a model for crystallographic temperature factors
48. Effect of anisotropy and anharmonicity on protein crystallographic refinement. An evaluation of molecular dynamics
49. Exploration of disorder in protein structures by X-ray restrained molecular dynamics
50. Steady-state kinetics of hydrolysis of dansyl-peptide substrates by leucine aminopeptidase
51. Traitement statistique des erreurs dans la determination des structures cristallines
52. Solvent content of protein crystals
53. Leucine aminopeptidase (bovine lens). Stability and size of subunits
54. Crystal orientation and X-ray pattern prediction routines for area-detector diffractometer systems in macromolecular crystallography
55. Leucinaminopeptidase: Untersuchungen zum aktiven Zentrum einer metallopeptidase
56. X-ray structure determination of (2S, 3R)-3-amino-2-hydroxy-4-phenylbutanoic acid, a new amino acid component of bestatin
57. Design of potent reversible inhibitors for thermolysin. Peptides containing zinc coordinating ligands and their use in affinity chromatography
58. Synthesis and structureactivity relationships of bestatin analogues, inhibitors of aminopeptidase B
59. Synthesis of sulfurcontaining analogues of bestatin. Inhibition of aminopeptidases by α-thiolbestatin analogues
60. Kinetic parameters for the slow binding of bestatin to beef lens leucine aminopeptidase(b1LAP)
61. Quantum theory of the structure and bonding in proteins
62. A systematic method for aligning double-focusing mirrors
63. RIBBON a stereo cartoon drawing program for proteins
64. Conformation of polypeptides and proteins
65. Improved Fourier coefficients for maps using phases from partial structures with errors
66. Crystallographic refinement and atomic models of two different forms of citrate synthase at 2·7 and 1·7 Å resolution
67. Crystal and molecular structure of bestatin and its implications regarding substrate binding to the active site of leucine aminopeptidase
68. Inhibition of aminopeptidases by amastatin and bestatin derivatives. Effect of inhibitor structure on slow-binding processes
69. Schematic drawings of protein structures
70. The structure of aconitase
71. Crystallization of methionine aminopeptidase from Escherichia coli
72. On the size of the active site in proteases I. Papain
73. Structure of myohemerythrin in the azidomet state at 1·7/1·3 Å resolution
74. Leucine aminopeptidase
75. Refined structure of yeast apo-enolase at 2·25 Å resolution
76. Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination
77. xerB, an Escherichia coli gene required for plasmid ColEI1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase
78. The structure of bestatin
79. Leucine aminopeptidase (bovine lens): An electron microscopic study
80. 2+ leucine aminopeptidase (EC 3.4.11.1)
81. Leucine aminopeptidase from bovine lens and hog kidney. Comparison using immunological techniques, electron microscopy, and X-ray diffraction
82. Comparison of leucine aminopeptidase from human lens, beef lens and kidney, and hog lens and kidney
83. Photoaffinity labeling of lens leucine aminopeptidase
84. Unbiased threedimensional refinement of heavy-atom parameters by correlation of origin-removed Patterson functions
85. 2+ to and on the activation of the enzyme
86. Leucine aminopeptidase (bovine lens). The relative binding of cobalt and zinc to leucine aminopeptidase and the effect of cobalt substitution on specific activity
87. Prolyl aminopeptidase from rat brain and kidney. Action on peptides and identification as leucine aminopeptidase
88. Bestatin, an inhibitor of aminopeptidase B, produced by actinomycetes
89. Zinc coordination, function, and structure of zinc enzymes and other proteins
90. Limited tryptic digestion of bovine eye lens leucine aminopeptidase
91. Metal binding stoichiometry and mechanism of metal ion modulation of the activity of porcine kidney leucine aminopeptidase
92. Dokl. Biophys
93. Purification and properties of an aminopeptidase from Escherichia coli
94. Bovine leucine aminopeptidase cDNA: isolation and characterization
95. Resolution of phase ambiguity in macromolecular crystallography
96. Strategies for extracting isomorphous arid anomalous signals
97. The slow, tight binding of bestatin and amastatin to aminopepti dases
98. The electronic stationary picture method for high speed measurement of reflection intensities from crystals with large unit cells