Introduction of intersubunit disulfide bonds in the membrane-distal region of the influenza hemagglutinin abolishes membrane fusion activity

Cell

Volumn 68, Issue 4, 1992, Pages 635-645

  Godley, L ^d   Pfeifer, J ^d   Steinhauer, D ^a   Ely, B ^a   Shaw, G ^b   Kaufmann, R ^b   Suchanek, E ^c   Pabo, C ^c   Skehel, J J ^a   Wiley, D C ^d   Wharton, S ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

^b PFIZER INC   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS HEMAGGLUTININ;

ANIMAL CELL; ARTICLE; CHO CELL; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CROSS LINKING; DISULFIDE BOND; ENDOSOME; INFLUENZA VIRUS; MEMBRANE FUSION; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; VIRUS HEMAGGLUTINATION; VIRUS MORPHOLOGY;

ANIMAL; BINDING SITES, ANTIBODY; BROMELAINS; CELL LINE; CYSTEINE; HAMSTERS; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HEMAGGLUTININS, VIRAL; HYDROGEN-ION CONCENTRATION; MEMBRANE FUSION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRYPSIN; VIRAL ENVELOPE PROTEINS;

ANIMALIA; INFLUENZA VIRUS;

EID: 0026560161     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(92)90140-8     Document Type: Article

References (38)

1. Substituted benzaldehydes designed to increase the oxygen affinity of human haemoglobin and inhibit the sickling of sickle erythrocytes
2. The expression and properties of polyoma virus middle T antigen in simian cells
3. Genetic basis of resistance to rimantadine emerging during treatment of influenza virus infection
4. Crystalline antigen from the influenza virus envelope
5. Testing topological models for the membrane penetration of the fusion peptide of influenza virus hemagglutinin
6. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
7. Analysis of the antigenicity of influenza haemagglutinin at the pH optimum for virus mediated membrane fusion
8. Fusion mutants of the influenza virus hemagglutinin glycoprotein
9. The receptor binding amd membrane fusion properties of influenza virus variants selected using anti-haemagglutinin monoclonal antibodies
10. Quaternary structure of influenza virus hemagglutinin after acid treatment
11. Membrane fusion activity of the influenza virus haemagglutinin
12. Antigenic determinants of influenza virus haemagglutinin. XI. Conformational changes detected by monoclonal antibodies
13. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene
14. The phosphorylation state of eukaryotic initiation factor 2 alters translational efficiency of specific mRNAs
15. Activation of influenza A virus by trypsin treatment
16. Cleavage of structural proteins during assembly of the head of bacteriophage T4
17. Enhancement of the infectivity of influenza A and B virus by proteolytic cleavage of the hemagglutinin polypeptide
18. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes
19. Transforming activity of polyoma virus middle-T antigen probed by site-directed mutagenesis
20. Computer-aided model-building strategies for protein design
21. A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells
22. Conformational changes and fusion activity of influenza hemagglutinin of the H2 and H3 subtypes: effects of acid pretreatment
23. Electron microscopy of the low pH structure of influenza virus haemagglutinin
24. Studies on the structure of the influenza virus haemagglutinin at the pH of membrane fusion
25. Hemolytic activity of influenza virus hemagglutinin glycoproteins activated in mildly acidic environments
26. Studies on the primary structure of the influenza virus hemagglutinin
27. Changes in the conformation of influenza virus hemagglutinin at the pH optimum of virus-mediated membrane fusion
28. Intermediates in influenza induced membrane fusion
29. Deacylation of the hemagglutinin of A/Aichi/2/68 has no effect on membrane fusion properties
30. Antigenic drift between the haemagglutinin of the Hong Kong influenza strains A/Aichi/2/68 and A/Victoria/3/75
31. Changes in the antigenicity of the haemagglutinin molecule of H3 influenza virus at acidic pH
32. The structure of a membrane fusion mutant of the influenza virus haemagglutinin
33. Studies of influenza hemagglutinin-mediated membrane fusion
34. Antipeptide antibodies detect steps in a protein conformational change: low pH activation of the influenza virus hemagglutinin
35. Membrane fusion proteins of enveloped animal viruses
36. The structure and function of the hemagglutinin membrane glycoprotein of influenza virus
37. Structure of the haemagglutinin membrane protein of influenza virus at 3 Å resolution
38. Monoclonal anti-hemagglutinin antibodies detect irreversible antigenic alterations that coincide with the acid activation of influenza virus A/PR/8/34-mediated hemolysis