Stabilization of substilisin E in organic solvents by site‐directed mutagenesis

Biotechnology and Bioengineering

Volumn 39, Issue 2, 1992, Pages 141-147

  Martinez, Pascal ^a   Van Dam, Mariana E ^a   Robinson, Amy C ^a   Chen, Keqin ^a   Arnold, Frances H ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

enzymes in organic solvents; protein engineering; protein stabilization; subtilisin E

Indexed keywords

AMINO ACIDS; SOLVENTS; THERMODYNAMICS;

BACILLUS SUBTILIS; DIMETHYLFORMAMIDE; FREE ENERGY; HYDROPHOBICITY; INTERNAL HYDROGEN BONDING; SUBTILISIN E;

ENZYMES;

N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; SUBTILISIN;

ARTICLE; BACILLUS SUBTILIS; ENZYME STABILITY; NONHUMAN; SITE DIRECTED MUTAGENESIS;

EID: 0026542996     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.260390204     Document Type: Article

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