The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion

Gene

Volumn 112, Issue 1, 1992, Pages 29-35

  Barnes, Wayne M ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

error rate; KlenTaq; lacZ gene; polyethylene glycol; polymerase chain reaction; recombinant DNA

Indexed keywords

DNA POLYMERASE; MACROGOL; RESTRICTION ENDONUCLEASE;

ARTICLE; ENZYME DEFECT; GENE STRUCTURE; MUTATION RATE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THERMOPHILIC BACTERIUM;

BASE SEQUENCE; BETA-GALACTOSIDASE; CLONING, MOLECULAR; DNA, RECOMBINANT; DNA-DIRECTED DNA POLYMERASE; LAC OPERON; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; TAQ POLYMERASE; THERMUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMUS AQUATICUS;

EID: 0026534432     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(92)90299-5     Document Type: Article

References (20)

1. Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion
2. High effiency transformation of E. coli by high voltage electroporation
3. Fidelity mechanisms in DNA replication
4. High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase
5. DNA polymerase fidelity and the polymerase chain reaction
6. Repeat unit sequence variation in minisatellites: a novel source of DNA polymorphism for studying variation and mutation by single molecule analysis
7. Sequence of the lacZ gene of Escherichia coli
8. Proteolytic cleavage of native DNA polymerase into two different catalytic fragments
9. Amino acid dependent control of DNA synthesis in bacteria and vegetative phage
10. A constant rate of spontaneous mutation in DNA-based microbes
11. Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction
12. Mutation spectra and the neutrality of mutations
13. Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and Vent DNA polymerases
14. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
15. Transfer of the °(argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion
16. Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide
17. Recessive lethals a new class of nonsense suppressors in Escherichia coli
18. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase
19. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification