Histidine-aromatic interactions in barnase. Elevation of histidine pKa and contribution to protein stability

Journal of Molecular Biology

Volumn 224, Issue 3, 1992, Pages 759-770

  Loewenthal, Ron ^a   Sancho, Javier ^a   Fersht, Alan R ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

electrostatic; engineering; helix dipole; protein folding; stability

Indexed keywords

AROMATIC AMINO ACID; HISTIDINE; MUTANT PROTEIN; PROTON; RIBONUCLEASE; SODIUM CHLORIDE; TRYPTOPHAN;

ARTICLE; CATALYSIS; CHEMICAL BINDING; ENZYME BINDING; ENZYME STABILITY; MOLECULAR INTERACTION; PH; PKA; PRIORITY JOURNAL; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; BASE SEQUENCE; COMPARATIVE STUDY; ENZYME STABILITY; HISTIDINE; HYDROGEN-ION CONCENTRATION; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN CONFORMATION; RIBONUCLEASES; STRUCTURE-ACTIVITY RELATIONSHIP; SUPPORT, NON-U.S. GOV'T; THERMODYNAMICS; TRYPTOPHAN;

EID: 0026525805     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-2836(92)90560-7     Document Type: Article

References (44)

1. 1H NMR spectroscopy
2. Two-dimensional spectroscopy. Application to nuclear magnetic resonance
3. Crystal structure of a barnase-d(GpC) complex at 1.9 Å resolution
4. Investigation of complex networks of spin-spin coupling by two dimensional NMR
5. Structure and Mechanism of Chymotrypsin
6. Aromatic interactions
7. Aromatic-aromatic interactions: a mechanism of protein structure stabilization
8. Amino aromatic interactions in proteins
9. 1H-nuclear magnetic resonance spectrum of barnase
10. Determination of the three dimensional solution structure of barnase using nuclear magnetic resonance spectroscopy
11. The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus)
12. A reversible thermal transition of the extracellular ribonuclease of Bacillus amyloliquefaciens
13. A two state conformational transition of the extracellular ribonuclease of Bacillus amyloliquefaciens (Barnase) induced by sodium dodecyl sulfate
14. Non-additivity in protein-protein interactions
15. Strength and co-operativity of contributions of surface salt bridges to protein stability
16. Investigation of exchange processes by two-dimensional NMR spectroscopy
17. Aromatic-aromatic interactions: free energy profiles for the benzene dimer in water, chloroform and liquid benzene
18. Contribution of hydrophobic interaction to protein stability
19. Energetics of complementary side-chain packing in a protein hydrophobic core
20. Alternating zinc fingers in the human male associated protein ZFY: refinement of the NMR structure of an even finger by selective deuterium labeling and implications for DNA recognition
21. Molscript a program to produce both detailed and schematic plots of protein structures
22. A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules
23. Fluorescence spectrum of barnase: contributions of three tryptophan residues and a histidine-related pH-dependence
24. + salt bridges in short peptides of de novo design
25. Molecular structure of a new family of ribonucleases
26. Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu58 → Ala
27. Enhanced protein thermostability from designed mutations that interact with α-helix dipoles
28. Novel thioether bond revealed by a 17 Å crystal structure of galactose oxidase
29. Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation
30. Hemoglobin as a receptor of drugs and peptides: X-ray studies of the stereochemistry of binding
31. Thermodynamic parameters for the helix-coil thermal transition of ribonuclease-S-peptide and derivatives from 1H-NMR data
32. Rational modification of enzyme catalysis by engineering surface charge
33. Stabilization of protein structure by interaction of α-helix dipole with a charged side chain
34. as and protein stability
35. Capping and α-helix stability
36. Estimating the contribution of engineered surface electrostatic interactions to protein stability using double-mutant cycles
37. Aromatic-aromatic interactions and protein stability: investigation by double mutant cycle
38. Fluorometric detection of histidine-tryptophan complexes in peptides and proteins
39. +
40. The interaction between phenylalanine rings in proteins
41. Crystallographic and NMR studies of the serine proteases
42. Structure of recombinant rheumatoid arthritic synovial fluid phospholipase A2 at 2.2 Å resolution
43. The quenching of tryptophan fluorescence by protonated and unprotonated imidazole
44. Determination of the excited state lifetimes of the tryptophan residues in barnase via multifrequency phasefluorometry of tryptophan mutants