Use of a high frequency ultrasound microscope to image the action of 2-nitroimidazoles in multicellular spheroids

British Journal of Cancer

Volumn 65, Issue 5, 1992, Pages 633-640

  Berube L R ^a   Harasiewicz, K ^b   Foster, F S ^a,b   Dobrowsky, E ^a   Sherar, M D ^a   Rauth, A M ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 METHYL 2 NITROSOIMIDAZOLE; NITROIMIDAZOLE DERIVATIVE;

ANIMAL CELL; ARTICLE; CELL SURVIVAL; COLONY FORMATION; CONTROLLED STUDY; DRUG CYTOTOXICITY; HYPOXIC CELL; IMAGING; IN VITRO STUDY; MICROSCOPY; MONOLAYER CULTURE; MULTICELLULAR SPHEROID; NONHUMAN; PRIORITY JOURNAL; RAT; TUMOR MODEL; ULTRASOUND;

ANIMAL; CELL DIVISION; CELL HYPOXIA; COLONY-FORMING UNITS ASSAY; IMIDAZOLES; MICROSCOPY; MUTAGENS; NITROIMIDAZOLES; NITROSO COMPOUNDS; TUMOR CELLS, CULTURED; ULTRASONICS;

EID: 0026510960     PISSN: 00070920     EISSN: 15321827     Source Type: Journal    
DOI: 10.1038/bjc.1992.137     Document Type: Article

References (24)

1. ACKER, H. (1984). Microenvironmental conditions in multicellular spheroids grown under liquid-overlay tissue culture conditions. Recent Results Cancer Res., 95, 116
2. BÉRUBÉ, L.R., FARAH, S., MCCLELLAND, R.A. & RAUTH, AM. (1991). Effect of l-methyl-2-nitrosoimidazole on intracellular thiols and calcium levels in Chinese hamster ovary cells. Biochem Pharmac., 42, 2153
3. CARLSSON, J., STÅLNACKE, C-G., ACKER, H., Haji-Karim, M., Nilsson, S. & Larsson, B. (1979). The influence of oxygen on viability and proliferation in cellular spheroids. Int. J. Radiat. Oncol. Biol. Phys., 5, 2011
4. CLARKE, P.R. & HILL, C.R. (1970). Physical and chemical aspects of ultrasonic disruption of cells. J. Acoust. Soc. Amer., 47, 649
5. DOBRUCKI, J.W., DENSAR, F., WALCZAK, T., WOODS, R.K., BACIC, G. & SWARTZ, H.M. (1990). Electron spin resonance microscopy of an in vitro tumour model. Br. J. Cancer, 61, 221
6. DOBRUCKI, J.W., SUTHERLAND, R.M. & SWARTZ, H.M. (1991). Non-perturbing test for cytotoxicity in isolated cells and spheroids, using electron paramagnetic imaging. Magn. Res. Med., 19, 42
7. FRANKO, A.J., FREEDMAN, H.I. & KOCH, C.J. (1984). Oxygen supply to spheroids in spinner and liquid-overlay culture. Recent Results Cancer Res., 95, 162
8. HALL, E.J. & Roizin-Towle, L. (1975). Hypoxic sensitizers: Radiobiological studies at the cellular level. Radiology, 117, 453
9. LUK, C. & SUTHERLAND, R.M. (1986). Influence of growth phase, nutrition and hypoxia on heterogeneity of cellular buoyant densities in in vitro tumor model systems. Int. J. Cancer, 37, 883
10. Mueller-Klieser, W. (1984). Microelectrode measurements of oxygen tension distributions in multicellular spheroids cultured in spinner flasks. Recent Results Cancer Res., 95, 134
11. NOSS, M.B., PANICUCCI, R., MCCLELLAND, R.A. & RAUTH, A M.(1988) Preparation, toxicity and mutagenicity of l-methyl-2- nitrosoimidazole. Biochem Pharmacol., 37, 2585
12. NOSS, M B., PANICUCCI, R., MCCLELLAND, R.A. & Rauth, A.M. (1988) l-methyl-2-nitrosoimidazole: Cytotoxic and glutathione depleting capabilities. Int. J. Radiat. Oncol. Biol. Phys., 16, 1015
13. PAVLIN, C.J., HARASIEWICZ, K., SHERAR, M.D. & FOSTER, F.S. (1991). Clinical use of ultrasound biomicroscopy. Ophthalmology, 98, 287
14. RAUTH, A.M. (1986). Pharmacology and toxicology of sensitizers: Mechanism studies. Int. J. Radiat. Oncol. Biol. Phys., 10, 1293
15. SHERAR, M.D. (1990). Ultrasound backscatter microscopy and its application to biological studies. PhD Thesis, Department of Medical Biophysics, University of Toronto
16. SHERAR, M.D., NOSS, M.B. & FOSTER, F.S. (1987). Ultrasound backscatter microscopy images the internal structure of living tumour spheroids. Nature, 330, 493
17. SHERAR, M.D. & FOSTER, F.S. (1987). A 100 MHz PVDF ultrasound microscope with biological applications. In Acoustical Imaging, Kessler, L.W. (ed.) 16, p.511, Plenum: New York
18. SHERAR, M.D. & FOSTER, F.S. (1988). Ultrasound backscatter microscopy. IEEE, 959
19. SHERAR, M.D. & FOSTER, F.S. (1989). The design and fabrication of high frequency polyvinylidene fluoride transducers. Ultrasonic imaging, 11, 75
20. SHERAR, M.D., STARKOSKI, B.G. & FOSTER, F.S. (1989). A 100 MHz B-scan ultrasound backscatter microscope. Ultrasonic Imaging, 11, 95
21. SILLERUD, L.O., FREYER, J.P., NEEMAN, M. & MATTINGLY, M.A.(1988) Proton NMR microscopy of multicellular tumor spheroid morphology. Mag. Reson. Med., 16, 380
22. SUTHERLAND, R.M. (1988). Cell and environment interactions in tumor microregions: The multicell spheroid model. Science, 240, 217
23. TER HAAR, G.R. & STRATFORD, I.J. (1982). Evidence for a nonthermal effect of ultrasound. Br. J. Cancer, 45, 172 (suppl. V)
24. WYLLIE, A.H. (1987). Apoptosis: Cell death in tissue regulation. J. Pathol., 153, 313