NAD(P)H:Quinone Oxidoreductase Gene Expression in Human Colon Carcinoma Cells: Characterization of a Mutation Which Modulates DT-Diaphorase Activity and Mitomycin Sensitivity

Cancer Research

Volumn 52, Issue 4, 1992, Pages 797-802

  Traver, Robert D ^a,b   Horikoshi, Tetsuro ^b   Danenberg, Kathleen D ^b   Stadlbauer, Thomas H W ^b   Ross, David ^c   Gibson, Neil W ^a,b  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,6 DICHLOROPHENOLINDOPHENOL; BETA ACTIN; COMPLEMENTARY DNA; CYTOSINE; MITOMYCIN; OXIDOREDUCTASE; PRIMER DNA; PROLINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE); SERINE; THYMIDINE;

AMINO ACID SEQUENCE; ANTIBIOTIC SENSITIVITY; ARTICLE; COLON CANCER; CONTROLLED STUDY; CYTOTOXICITY; DNA SEQUENCE; ENZYME ACTIVITY; GENE CONTROL; GENE EXPRESSION; GENE MUTATION; HUMAN; HUMAN CELL; NUCLEIC ACID BASE SUBSTITUTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; TUMOR CELL LINE;

ACTINS; BASE SEQUENCE; COLONIC NEOPLASMS; DNA, NEOPLASM; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMAN; KINETICS; MITOMYCIN; MOLECULAR SEQUENCE DATA; NAD(P)H DEHYDROGENASE (QUINONE); OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION; RNA, NEOPLASM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED;

EID: 0026505943     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Siegel, D., Gibson, N. W., Preusch, P. C., and Ross, D. Metabolism of diaziquone by NAD(P)H:(quinone acceptor) oxidoreductase (DT-diaphorase): role in diaziquone-induced DNA damage and cytotoxicity in human colon carcinoma cells. Cancer Res., SO:7293-7300, 1990.
2. Siegel, D., Gibson, N. W., Preusch, P. C., and Ross, D. Metabolism of mitomycin C by DT-diaphorase: role in mitomycin C-induced DNA damage and cytotoxicity in human colon carcinoma cells. Cancer Res., 50: 7483-7489, 1990.
3. Egbertson, M., and Danishefsky, S. Modeling of the electrophilic activation of mitomycins: chemical evidence for the intermediacy of a mitosene semiquinone as the active electrophile. J. Am. Chem. Soc., 109: 2204-2205, 1987.
4. Doroshow, J. Role of hydrogen peroxide and hydroxyl radical formation in the killing of Ehrlich cancer cell by antitumor qui±ones.Proc. Natl. Acad. Sci. USA, W: 4514-4518, 1986.
5. Dusre, L., Covey, J., Collins, C., and Sinha, B. DNA damage, cytotoxicity and free radical formation by mitomycin C in human cells. Chem. Biol. Interact., 77:63-78, 1989.
6. Butler, J., Hoey, B., and Swallow, A. Reactions of the semiquinone free radicals of anti-tumor agents with oxygen and iron complexes. FEBS Lett., 182: 95-98, 1985.
7. Lind, C., Hochstein, P., and Ernster, L. DT-diaphorase as a quinone reduc ase. A cellular control device against semiquinone and Superoxide radical formation. Arch. Biochem. Biophys., 216: 178-185, 1982.
8. Ross, D., Siegel, D., Preusch, P. C., and Gibson, N. W. Bioreductive activation of mitomycin C by DT-diaphorase. Proc. Am. Assoc. Cancer Res., 32:320, 1991.
9. Ernster, L. DT-diaphorase. Methods Enzymol., 10: 309-317, 1967.
10. Benson. A., Humkler, M., and Talalay, P. Increase of NAD(P)H:quinone reducaseby dietary antioxidants: possible role in protection against carcinogenesis and toxicity. Proc. Natl. Acad. Sci. USA, 77: 5216-5220, 1980.
11. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Biochemistry, 72:248-254, 1976.
12. Chomzynski, P., and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162: 156-159, 1987.
13. Huang, M. N., and High. K. A. A simple and fast method to extract RNA from tissue culture cells. Biotechniques, 9: 711-712, 1990.
14. Horikoshi, T., Danenberg, K. D., Stadibauer, T. H. W., Volkenandt, M., Shea, L. C. C., Aigner, K., Gustavsson, B., Leichman, L., Frosing, R., Ray, M., Gibson, N. W., and Danenberg, P. V. Quantitation of thymidylate synthase, dihydrofolate reducuase, and DT-diaphorase gene expression in human tumors using the polymerase chain reaction. Cancer Res., in press, 1992.
15. Danenberg, P. V., Horikoshi, T., Volkenandt, M., Danenberg, K. D., Shea, L. C. C., Dicker, A. P., and Berlino, J. R. Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s). Nucleic Acids Res., in press, 1992.
16. Allard, M. W., Ellsworth, D. L., and Honeycutt. R. L. The production of single-stranded DNA suitable for sequencing using the polymerase chain reaction. Biotechniques, 10: 24-26, 1991.
17. Schuurman, R., and Keulen, W. Modified protocol for DNA sequence analysis using sequenase 2.0. Biotechniques, 10:185, 1991.
18. Stoflet, E. S., Koeberl, D. D., Sarkar, G., and Sommer, S. S. Genomic amplification with transcript sequencing. Science (Washington DC), 239: 491-494, 1988.
19. Jaiswal, A. K., McBride. O. W., Adensnik, M., and Nebert, D. W. Human dioxin-inducible cytosolic NAD(P)H:menadione oxidoreductase. J. Biol. Chem., 263: 13572-13578, 1988.
20. Watson, M. A., and Milbrandt, J. Expression of the nerve growth factorregulated NGFI-A and NGFI-B genes in the developing rat. Development, 110: 173-183, 1990.
21. Noonan, K. E., Beck, C., Holzmayer, T. A., Chin, J. E., Wunder, J. S., Andrulis, I. L., Gazdar, A. F., Willman, C. L., Griffith, B., Von Hoff, D. D., and Roninson, I. B. Quantitative analysis of mdrl (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc. Natl. Acad. Sci. USA, 87: 7160-7164, 1990.
22. Murphy, L. D., Herzog, C. E., Rudick, J. B., Fojo, A. T., and Bates, S. E. Use of the polymerase chain reaction in the quantitation of mdr-1 gene expression. Biochemistry, 29: 10351-10356, 1990.
23. Wang, A. M., Doyle, M. V., and Mark, D. F. Quantitation of mRNA by the polymerase chain reaction. Biochemistry, 86: 9717-9721, 1989.
24. Scanlon, K. J., and Kashani-Sabet, M. Utility of the polymerase chain reaction in detection of gene expression in drug-resistant human tumors. J. Clin. Lab. Anal. 3: 323-329, 1989.
25. Doyle, A., Martin. W. J., Fua, K., Gasdar, A., Carney, C., Martin S. E., Linnoila, I., Cuttitta, F., Mulshine. J., Bunn, P., and Minna, J. Markedly decreased expression of class 1 histocompatibility antigens, protein, and mRNA in human small-cell lung cancer. J. Exp. Med., 161: 1135-1151, 1985.
26. Willson, J. K. V., Long. B. H., Marks, M. E., Brattain, D. E., Wiley, J. E., and Brattain, M. G. Mitomycin C resistance in a human colon carcinoma cell line associated with cell surface protein alterations. Cancer Res., 44: 5880-5885, 1984.
27. Kozak, M. Complication and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res., 12: 857-872, 1984.
28. Richardson. J. S., and Richardson. D. C. Principles and patterns of protein conformation. In: G. D. Fasman (ed.). Prediction of Protein Structure and the Principles of Protein Conformation, pp. 48-49. New York: Plenum Press, 1989.
29. Myszka, D. G., and Swenson, R. P. Synthesis of photoaffinity probe 3-(pazidobenzyl)-4-hydroxycoumarin and identification of the dicoumarol bind ing site in rat liver NAD(P)H:quinone reducase EC 1.6.99.2). J. Biol. Chem., 266: 4789-4797, 1991.
30. Cornelisse, C. J., and Schor, N. A. Biochemical and quantitative histochemical study of reduced pyridine nucleotide dehydrogenation by human colonie carcinomas. Cancer Res. 43: 4850-4855, 1983.