A rapid method for localized mutagenesis of yeast genes

Yeast

Volumn 8, Issue 2, 1992, Pages 79-82

  Muhlrad, Denise ^a   Hunter, Rosalyn ^a   Parker, Roy ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

PCR; Saccharomyces cerevisiae; Site directed mutagenesis

Indexed keywords

ARTICLE; ESCHERICHIA COLI; GENETIC TRANSFORMATION; MUTAGENESIS; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

DNA, FUNGAL; GENES, FUNGAL; MUTAGENESIS, SITE-DIRECTED; PEPTIDES; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 0026501752     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.320080202     Document Type: Article

References (12)

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2. A family of versatile centromeric vectors designed for use in the sectoring‐shuffle mutagenesis assay in Saccharomyces cerevisiae
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4. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique A
5. Plasmid construction by homologous recombination in yeast
6. Multiple roles for the U6/U4 snRNP in the splicing reaction
7. The a‐factor pheromone of Saccharomyces cerevisiae is essential for mating
8. A general method for saturation mutagenesis of cloned DNA fragments
9. The “megaprimer” method of site‐directed mutagenesis
10. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
11. Strategies and applications of in vitro mutagenesis
12. In vitro mutagenesis and plasmid shuffing: from cloned gene to mutant yeast