A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome

Cell

Volumn 71, Issue 5, 1992, Pages 803-817

  Madhani, Hiten D ^a   Guthrie, Christine ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ARTICLE; BASE PAIRING; CATALYSIS; GENE MUTATION; GENE REARRANGEMENT; GENE REPRESSION; GENE SEQUENCE; GENETIC CONSERVATION; INTRON; MUTAGENESIS; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; RNA SPLICING; RNA STRUCTURE; SACCHAROMYCES CEREVISIAE; SPLICEOSOME;

BASE SEQUENCE; GENETIC COMPLEMENTATION TEST; HYDROGEN BONDING; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PHYLOGENY; RNA SPLICING; RNA, FUNGAL; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE; SPLICEOSOMES; STRUCTURE-ACTIVITY RELATIONSHIP; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

SACCHAROMYCES CEREVISIAE;

EID: 0026486883     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(92)90556-R     Document Type: Article

References (63)

1. Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes
2. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics
3. Human and human-yeast chimeric U6 snRNA genes identify structural elements required for expression in yeast
4. Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing
5. Spliceosomal RNA U6 is remarkably conserved from yeast to mammals
6. Splicing a spliceosomal RNA
7. The generality of self-splicing RNA: relationship to nuclear mRNA splicing
8. Spliceosome assembly in yeast
9. Genetic evidence for base pairing between U2 and U6 snRNAs in mammalian mRNA splicing
10. A family of versatile centromeric vectors for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae
11. Two domains of yeast U6 small nuclear RNA required for both steps of nuclear precursor messenger RNA splicing
12. In vitro assembly of yeast U6 snRNPs: a functional assay
13. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing
14. Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein
15. Spliceosomal snRNAs
16. An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis
17. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle
18. Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles
19. Splice site selection and the role of the lariat in a group II intron
20. Multiple exon-binding sites in class II self-splicing introns
21. Group II intron domain 5 facilitates a trans-splicing reaction
22. Intron splicing: a conserved internal signal in the introns of Drosophila pre-mRNAs
23. Group II introns deleted for multiple substructures retain self-splicing activity
24. Preparation of high molecular weight RNA
25. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes
26. Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein
27. Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2′-OMe RNA
28. Multiple roles for U6 snRNA in the splicing pathway
29. Comparative and functional anatomy of group II catalytic introns: a review
30. Limited functional equivalence of phylogenetic variation in small nuclear RNA: yeast U2 RNA with altered branch point complementarity inhibits splicing and produces a dominant lethal phenotype
31. Site-specific modification of pre-mRNA: the 2' hydroxyl groups at the splice sites
32. An intron in the genes for U3 small nucleolar RNAs of the yeast Saccharomyces cerevisiae
33. Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage
34. U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites
35. Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA
36. An essential yeast snRNA with a U5-like domain is required for splicing in vivo
37. A U-rich tract enhances usage of an alternative 3′ splice site in yeast
38. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes
39. Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen crosslinking
40. Small nuclear RNAs from budding yeasts: phylogenetic comparisons reveoi extensive size variation
41. Association of U6 snRNA with the 5′-splice site region of pre-mRNA in the spliceosome
42. Self splicing of group II introns in vitro: mapping of the branch point and mutational inhibition of lariat formation
43. D-E-A-D protein family of putative RNA helicases
44. Vectors for expression of cloned genes in yeast regulation overproduction and underproduction
45. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
46. Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs
47. On the origins of RNA splicing and introns
48. Five easy pieces
49. Two conserved domains of yeast U2 snRNA are separated by 945 nonessential nucleotides
50. Human U2 snRNA can function in pre-mRNA splicing in yeast
51. An essential snRNA from S. cerevisiae has properties predicted for U4. including interaction with a U6-like snRNA
52. The gene for the U6 small nuclear RNA in fission yeast has an intron
53. mRNA-type introns in U6 small nuclear RNA genes: implications for the catalysis in pre-mRNA splicing
54. Expression of a β-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast
55. Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes
56. Roles of U4 and U6 snRNAs in the assembly of splicing complexes
57. Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing
58. Reconstituted mammalian U4/U6 snRNP complements splicing: a mutational analysis
59. Mammalian pre-mRNA branch site selection by U2 snRNP involves base-pairing
60. Base-pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA
61. U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction
62. A compensatory base change in human U2 snRNA can suppress a branch site mutation