High-level expression in Escherichia coli of enzymatically active fusion proteins containing the domains of mammalian cytochromes P450 and NADPH-P450 reductase flavoprotein

Proceedings of the National Academy of Sciences of the United States of America

Volumn 89, Issue 22, 1992, Pages 10817-10821

  Fisher, C W ^a   Shet, M S ^a   Caudle, D L ^a   Martin Wixtrom, C A ^a   Estabrook, R W ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

oxidation; hybrid protein; steroid 17 hydroxylation

Indexed keywords

17 HYDROXYPREGNENOLONE; ARACHIDONIC ACID; COMPLEMENTARY DNA; CYTOCHROME B5; CYTOCHROME P450; FLAVOPROTEIN; HYBRID PROTEIN; LAURIC ACID; OXYGENASE; PHOSPHOLIPID; PRASTERONE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE; STEROID 17,20 LYASE;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CATTLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; FATTY ACID METABOLISM; FATTY ACID OMEGA OXIDATION; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; RAT; SPECTROPHOTOMETRY; STEROID METABOLISM;

AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; CATTLE; CELL MEMBRANE; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; KINETICS; LIVER; MOLECULAR SEQUENCE DATA; NADPH-FERRIHEMOPROTEIN REDUCTASE; OLIGODEOXYRIBONUCLEOTIDES; POLYMERASE CHAIN REACTION; PREGNENOLONE; PROGESTERONE; RATS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

ANIMALIA; BOS TAURUS; BOVINAE; ESCHERICHIA COLI; MAMMALIA;

EID: 0026485793     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.89.22.10817     Document Type: Article

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