Heat-induced aggregation of recombinant erythropoietin in the intact and deglycosylated states as monitored by gel permeation chromatography combined with a low-angle laser light scattering technique

Journal of Biochemistry

Volumn 112, Issue 5, 1992, Pages 700-706

  Endo, Yoshiyuki ^a   Nagai, Hiroshi ^a   Watanabe, Yoshihiko ^a   Ochi, Kiyoshige ^a   Takagi, Toshio ^b  

^a CHUGAI PHARMACEUTICAL CO LTD   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; CARBOHYDRATE; RECOMBINANT ERYTHROPOIETIN; SODIUM CHLORIDE;

ALKALINITY; ANIMAL CELL; ARTICLE; COVALENT BOND; DEGLYCOSYLATION; GEL PERMEATION CHROMATOGRAPHY; HEATING; HUMAN; LASER; LIGHT SCATTERING; MONITORING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY;

ANIMAL; CARBOHYDRATES; CHO CELLS; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; ERYTHROPOIETIN; GLYCOSYLATION; HAMSTERS; HEAT; HUMAN; LASERS; LIGHT; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SALTS; SCATTERING, RADIATION; TEMPERATURE; TRANSFECTION;

EID: 0026440051     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a123961     Document Type: Article

References (35)

1. Jacobson, L.O., Goldwasser, E., Fried, W., & Plzak, L. (1957) Nature 179, 633-634
2. Fried, W. (1972) Blood 40, 671-677
3. Zanjani, E.D., Ascensao, J.L., McGlave, P.B., Banisadre, M., & Ash, R.C. (1981) J. Clin. Invest 67, 1183-1188
4. Schuster, S.J., Wilson, J.H., Erslev, A.J., & Caro, J. (1987) Blood 70, 316-318
5. Filmanowicz, E. & Gurne, C.W. (1961) J. Lab. Clin. Med. 67, 65-72
6. Miyake, T., Kung, C.K.H., & Goldwasser, E. (1977) J. Biol. Chem. 252, 5558-5564
7. Lin, F.K., Suggs, S., Lin, CH., Brown, J.K., Smalling, R., Egrie, J.C, Chen, K.K., Fox, G.M., Martin, F., Stabinsky, Z., Badrawi, S.M., Lai, P.H., & Goldwasser, E. (1985) Proc. Natl. Acad. Sci. USA 82, 7680-7684
8. Recny, M.R., Scoble, H.A., & Kim, Y. (1987) J. Biol Chem, 262, 17156-17163
9. Takeuchi, M., Takasakd, S., Miyazaki, H., Kato, T., Hoshi, S., Kochibe, N., & Kobata, A. (1988) J. Biol Chem, 263, 3667-3671
10. Sasaki, H., Bothner, B., Dell, A., & Fukuda, M. (1987) J. Biol Chem, 262, 12059-12076
11. Sasaki, H., Ochi, N., Dell, A., & Fukuda, M. (1988) Biochemistry 27, 8618-8626
12. Davis, M.D., Arakawa, T., Strickland, T.W., & Yphantis, D.A. (1987) Biochemistry 26, 2633-2638
13. Takagi, T. (1985) in Progress in HPLC (Parvez, H. et al., eds.) Vol. 1, pp. 27-41, VNU Science Press BV, Utrecht
14. Takagi, T. (1990) J. Chromatogr. 806, 409-416
15. Sato, Y., Ishikawa, N., & Takagi, T. (1990) J. Chromatogr. 507, 25-31
16. Kato, A. & Takagi, T. (1987) J. Agric. Food Chem 35, 633-637
17. Jacobs, K., Shoemaker, C, Rudersdolf, R., Neill, S.D., Kaufman, R., Mufson, A., Seehra, J., Jones, S.S., Hewick, R., Fritech, E.F., Kawakita, M., Shimizu, T., & Miyake, T. (1985) Nature 313, 806-810
18. Kupke, D.W. & Dorrier, T.E. (1978) Methods Enzymol. 48, 156-162
19. Hayashi, Y., Matsui, H., & Takagi, T. (1989) Methods Enzymol 172, 514-528
20. Endo, Y., Takeda, A., Kohara, F., Sayama, T., Nagai, H., Watanabe, Y., & Ochi, K. (1990) Rinshou-iyaku (in Japanese) 6, 21-27
21. Greenfield, N. & Fasman, G.D. (1969) Biochemistry 8, 4108-4116
22. Maezawa, S. & Takagi, T. (1983) J. Chromatogr. 280, 124-130
23. Lowy, P.H., Keighley, G., & Borsook, H. (1960) Nature 185, 102-103
24. Channing, CP., Sasaki, C.N., & Bahl, O.P. (1978) Endocrinology 103, 341-348
25. Kalyan, N.K. & Bahl, O.P. (1983) J. Biol. Chem. 258, 67-71
26. Sairam, M.R. (1980) Arch, Biochem, Biophys. 204, 199-206
27. Weintraub, B.D., Stannard, B.S., Linnekin, D., & Marshall, M. (1980) J. Biol. Chem. 258, 5715-5723
28. Dordal, M.S., Wang, F.F., & Goldwasser, E. (1985) Endocrinology 116, 2293-2299
29. Imai, N., Higuchi, M., Kawamura, A., Tomonoh, K., Oh-eda, M., Fujiwara, M., Shimonaka, Y., & Ochi, N. (1990) Eur. J. Biochem 194, 457-462
30. Goldwasser, E., Rung, C.K.H., & Eliason, J. (1974) J. Biol Chem, 249, 4202-4206
31. Teuda, E., Kawanishi, G., Ueda, M., Masuda, S., & Sasaki, R. (1990) Eur. J. Biochem. 188, 405-411
32. Morell, A.G., Gregoriadis, G., & Scheinberg, LH. (1971) J. Biol Chem, 246, 1461-1467
33. Fukuda, M.N., Sasaki, H., Lopez, L., & Fukuda, M. (1989) Blood 73, 84-89
34. Dube, S., Fisher, J.W., & Powell, J.S. (1988) J. Biol Chem. 283, 17516-17521
35. Kozulic, B., Leustek, I., Pavlovic, B., Mildner, P., & Barbaric, S. (1987) Appl Biochem. Biotech. 15, 265-278