A component of calcium-activated potassium channels encoded by the Drosophila slo locus

Science

Volumn 253, Issue 5019, 1991, Pages 551-555

  Atkinson, Nigel S ^a,b   Robertson, Gail A ^b   Ganetzky, Barry ^b  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; DNA;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHROMOSOME ABERRATION; CHROMOSOME DELETION; CHROMOSOME TRANSLOCATION; COMPARATIVE STUDY; DROSOPHILA; DRUG EFFECT; EXON; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEIC ACID HYBRIDIZATION; PHENOTYPE; PHYSIOLOGY; POTASSIUM CHANNEL; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMAL; CALCIUM; CHROMOSOME ABERRATIONS; CHROMOSOME DELETION; CLONING, MOLECULAR; COMPARATIVE STUDY; DNA; DROSOPHILA; EXONS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; PHENOTYPE; POTASSIUM CHANNELS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC; TRANSLOCATION (GENETICS);

EID: 0026413976     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1857984     Document Type: Article

References (56)

1. B. Hille, Ionic Channels of Excitable Membranes (Sinauer, Sunderland, MA, 1984).
2. B. Rudy, Neuroscience 25, 729 (1988); R. Latorre, A. Oberhauser, P. Labarca, O. Alvarez, Annu. Rev. Physiol. 51, 385 (1989).
3. B. Rudy, Neuroscience 25, 729 (1988); R. Latorre, A. Oberhauser, P. Labarca, O. Alvarez, Annu. Rev. Physiol. 51, 385 (1989).
4. A. Kamb, L. E. Iverson, M. A. Tanouye, Cell 50, 405 (1987); D. M. Papazian, T. L. Schwarz, B. L. Temple, Y. N. Jan, L. Y. Jan, Science 237, 749 (1987); O. Pongs et al., EMBO J. 7, 1087 (1988).
5. A. Kamb, L. E. Iverson, M. A. Tanouye, Cell 50, 405 (1987); D. M. Papazian, T. L. Schwarz, B. L. Temple, Y. N. Jan, L. Y. Jan, Science 237, 749 (1987); O. Pongs et al., EMBO J. 7, 1087 (1988).
6. A. Kamb, L. E. Iverson, M. A. Tanouye, Cell 50, 405 (1987); D. M. Papazian, T. L. Schwarz, B. L. Temple, Y. N. Jan, L. Y. Jan, Science 237, 749 (1987); O. Pongs et al., EMBO J. 7, 1087 (1988).
7. B. Rudy, C. Kentros, E. Vega-Saenz de Miera, J. Mol. Cell. Neurosci. 2, 89 (1991).
8. L. Salkoff, Nature 302, 249 (1983).
9. T. Elkins, B. Ganetzky, C.-F. Wu, Proc. Natl. Acad. Sci. U.S.A. 83, 8415 (1986).
10. S. Singh and C.-F. Wu, Neuron 2, 1325 (1989); A. Komatsu, S. Singh, P. Rathe, C.-F. Wu, ibid. 4, 313 (1990).
11. S. Singh and C.-F. Wu, Neuron 2, 1325 (1989); A. Komatsu, S. Singh, P. Rathe, C.-F. Wu, ibid. 4, 313 (1990).
12. B. S. Pallotta, K. L. Magelby, J. N. Barrett, Nature 293, 471 (1981); R. Latorre, C. Vergara, C. Hidalgo, Proc. Natl. Acad. Sci. U.S.A. 79, 805 (1982); C. Miller, E. Moczydlowski, R. Latorre, M. Phillips, Nature 313, 316 (1985).
13. B. S. Pallotta, K. L. Magelby, J. N. Barrett, Nature 293, 471 (1981); R. Latorre, C. Vergara, C. Hidalgo, Proc. Natl. Acad. Sci. U.S.A. 79, 805 (1982); C. Miller, E. Moczydlowski, R. Latorre, M. Phillips, Nature 313, 316 (1985).
14. B. S. Pallotta, K. L. Magelby, J. N. Barrett, Nature 293, 471 (1981); R. Latorre, C. Vergara, C. Hidalgo, Proc. Natl. Acad. Sci. U.S.A. 79, 805 (1982); C. Miller, E. Moczydlowski, R. Latorre, M. Phillips, Nature 313, 316 (1985).
15. M. Saito and C.-F. Wu, J. Neurosci. 11, 2135 (1991).
16. N. S. Atkinson, G. A. Robertson, B. Ganetzky, Soc. Neurosci. Abstr. 15, 541 (1989); G. A. Robertson, N. S. Atkinson, B. Ganetzky, ibid. 16, 670 (1990); _, Biophys. J. 59, 196a (1990).
17. N. S. Atkinson, G. A. Robertson, B. Ganetzky, Soc. Neurosci. Abstr. 15, 541 (1989); G. A. Robertson, N. S. Atkinson, B. Ganetzky, ibid. 16, 670 (1990); _, Biophys. J. 59, 196a (1990).
18. N. S. Atkinson, G. A. Robertson, B. Ganetzky, Soc. Neurosci. Abstr. 15, 541 (1989); G. A. Robertson, N. S. Atkinson, B. Ganetzky, ibid. 16, 670 (1990); _, Biophys. J. 59, 196a (1990).
19. D. L. Lindsley and E. H. Grell, Genetic Variations of Drosophila melanogaster (Publication No. 627, Carnegie Institution of Washington, Washington, DC, 1968).
20. + identified the mutagenized chromosome.
21. 4.
22. E. Knust, K. Tietze, J. A. Campos-Ortega, EMBO J. 6, 4113 (1987).
23. W. Bender, P. Spierrer, D. S. Hogness, J. Mol. Biol. 168, 17 (1983).
24. + tail. DNA was sequenced in M13 and pBluescript (Stratagene) vectors by the dideoxy chain termination method [F. Sanger, S. Nicklen, A. R. Coulson, Proc. Natl. Acad. Sci. U.S.A. 74, 5463 (1977)] with a Sequenase kit (United Biochemical Corp.). All cDNAs were sequenced on both strands. Regions showing GC compression were sequenced with deoxyinosine triphosphate (DITP). Sequence analyses were performed with software from the Genetics Computer Group [ J. Devereux, P. Haeberli, O. Smithies, Nucleic Acids Res. 12, 387 (1984)] and the Intelligenetics Molecular Biology Software System (IntelliGenetics, Inc.).
25. + tail. DNA was sequenced in M13 and pBluescript (Stratagene) vectors by the dideoxy chain termination method [F. Sanger, S. Nicklen, A. R. Coulson, Proc. Natl. Acad. Sci. U.S.A. 74, 5463 (1977)] with a Sequenase kit (United Biochemical Corp.). All cDNAs were sequenced on both strands. Regions showing GC compression were sequenced with deoxyinosine triphosphate (DITP). Sequence analyses were performed with software from the Genetics Computer Group [ J. Devereux, P. Haeberli, O. Smithies, Nucleic Acids Res. 12, 387 (1984)] and the Intelligenetics Molecular Biology Software System (IntelliGenetics, Inc.).
26. + channel has been noted [K. Loughney, R. Kreber, B. Ganetzky, Cell 58, 1143 (1989)].
27. + channel has been noted [K. Loughney, R. Kreber, B. Ganetzky, Cell 58, 1143 (1989)].
28. + channel has been noted [K. Loughney, R. Kreber, B. Ganetzky, Cell 58, 1143 (1989)].
29. N. J. Proudfoot and G. G. Brownlee, Nature 263, 211 (1976).
30. J. Kyte and R. F. Doolittle, J. Mol. Biol. 157, 105 (1982).
31. W. A. Catterall, Science 242, 50 (1988); W. Stuhmer et al., Nature 339, 597 (1989); D. M. Papazian, L. C. Timpe, Y. N. Jan, L. Y. Jan, ibid. 349, 305 (1991).
32. W. A. Catterall, Science 242, 50 (1988); W. Stuhmer et al., Nature 339, 597 (1989); D. M. Papazian, L. C. Timpe, Y. N. Jan, L. Y. Jan, ibid. 349, 305 (1991).
33. W. A. Catterall, Science 242, 50 (1988); W. Stuhmer et al., Nature 339, 597 (1989); D. M. Papazian, L. C. Timpe, Y. N. Jan, L. Y. Jan, ibid. 349, 305 (1991).
34. L. Y. Jan and Y. N. Jan, ibid. 345, 672 (1990).
35. R. MacKinnon, L. Heginbotham, T. Ahramson, Neuron 5, 767 (1990), H. A. Hartmann et al., Science 251, 942 (1991).
36. R. MacKinnon, L. Heginbotham, T. Ahramson, Neuron 5, 767 (1990), H. A. Hartmann et al., Science 251, 942 (1991).
37. G. Yellen, M. E. Jurman, T. Abramson, R. MacKinnon, Science 251, 939 (1991).
38. A. J. Yool and T. Schwarz, Nature 349, 700 (1991).
39. R. MacKinnon and G. Yellen, Science 250, 276 (1990).
40. R. Drysdale, J. Warmke, R. Kreber, B. Ganetsky, Genetics 127, 497 (1991).
41. J. Warmke, R. Drysdale, B. Ganetzky, Science 252, 1560 (1991); Y. Zhong and C.-F. Wu, ibid., p. 1562.
42. J. Warmke, R. Drysdale, B. Ganetzky, Science 252, 1560 (1991); Y. Zhong and C.-F. Wu, ibid., p. 1562.
43. M. Gorczyca and C.-F. Wu, J. Mem. Biol. 121, 237 (1991).
44. + channels. For example, when aligned with the S5 segment of Sh there are ten identities and five conservative substitutions among 21 residues. In contrast, the S5 with S5 alignment yields five identities and three conservative substitutions and the S6 with S6 alignment yields four identities and four conservative substitutions. The significance of this alignment between hydrophobic domains that do not occur at corresponding positions is not known.
45. R. M. Tufty and R. Kretsinger, Science 187, 167 (1975); R. H. Kretsinger, Cold Spring Harb. Symp. Quant. Biol. 52, 499 (1987).
46. R. M. Tufty and R. Kretsinger, Science 187, 167 (1975); R. H. Kretsinger, Cold Spring Harb. Symp. Quant. Biol. 52, 499 (1987).
47. 15-20-K [M. P. Kamps, S. S. Taylor, B. M. Sefton, Nature 310, 589 (1984)].
48. C. H. Gelband, S. D. Silberberg, K. Groschner, C. van Breemen, Proc. R. Soc. London Ser. B 242, 23 (1990).
49. T. L. Schwarz, B. L. Tempel, D. M. Papazian, Y. N. Jan, L. Y. Jan, Nature 331, 137 (1988); L. C. Timpe, Y. N. Jan, L. Y. Jan, Neuron 1, 659 (1988).
50. T. L. Schwarz, B. L. Tempel, D. M. Papazian, Y. N. Jan, L. Y. Jan, Nature 331, 137 (1988); L. C. Timpe, Y. N. Jan, L. Y. Jan, Neuron 1, 659 (1988).
51. Chromosomal in situ hybridization was performed as described [W. Engels, C. Preston, P. Thompson, W. Eggleston, Focus 8, 6 (1986)] except that we used sulfonated (Chemiprobe kit, FMC BioProducts) rather than biotinylated probes.
52. Consensus recognition sequences were (R,K)-(R,K)-X-S or (R,K)-(R,K)-X-X-S for adenosine 3′, 5′-monophosphate (cAMP)-dependent phosphorylation sites [B. E. Kemp, D. J. Graves, E. Benjamini, E. G. Krebs, J. Biol. Chem. 252, 4888 (1977)] and (S,T)-X-(R,K) for protein kinase C (PKC)-dependent phosphorylation [J. R. Woodget, K. L. Gould, T. Hunter, Eur. J. Biochem. 161, 177 (1986); A. Kishimoto et al., J. Biol. Chem. 260, 12492 (1985)].
53. Consensus recognition sequences were (R,K)-(R,K)-X-S or (R,K)-(R,K)-X-X-S for adenosine 3′, 5′-monophosphate (cAMP)-dependent phosphorylation sites [B. E. Kemp, D. J. Graves, E. Benjamini, E. G. Krebs, J. Biol. Chem. 252, 4888 (1977)] and (S,T)-X-(R,K) for protein kinase C (PKC)-dependent phosphorylation [J. R. Woodget, K. L. Gould, T. Hunter, Eur. J. Biochem. 161, 177 (1986); A. Kishimoto et al., J. Biol. Chem. 260, 12492 (1985)].
54. Consensus recognition sequences were (R,K)-(R,K)-X-S or (R,K)-(R,K)-X-X-S for adenosine 3′, 5′-monophosphate (cAMP)-dependent phosphorylation sites [B. E. Kemp, D. J. Graves, E. Benjamini, E. G. Krebs, J. Biol. Chem. 252, 4888 (1977)] and (S,T)-X-(R,K) for protein kinase C (PKC)-dependent phosphorylation [J. R. Woodget, K. L. Gould, T. Hunter, Eur. J. Biochem. 161, 177 (1986); A. Kishimoto et al., J. Biol. Chem. 260, 12492 (1985)].
55. M. Noda et al., Nature 320, 188 (1986).
56. We dedicate this paper to the memory of Tom Elkins, our colleague and friend whose pioneering studies provided the groundwork for this analysis. We thank C. Petersen, and R. Kreber for technical assistance; F. Blattner, L. Marr, and members of the Blattner lab for help with sequencing; E. Knust and J. Campos-Ortega for providing stocks and clones; J. Tamkun, T. Schwarz, L. Jan, Y. N. Jan, B. Hamilton, and E. Meyerowitz for providing libraries; and our colleagues for advice and comments on the manuscript. Supported by NIH grant NS15390, the Markey Charitable Trust, and a Klingenstein Fellowship to B.G. N.S.A. received support from NIH grant T32 GM07131 and the Muscular Dystrophy Association. G.A.R. received support from the American Heart Association of Wisconsin, NIH grant T32 GM07131, and an NIH postdoctoral fellowship. This is paper number 3194 from the Laboratory of Genetics, University of Wisconsin-Madison.