Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: Integration, expression and ultrastructural changes

Journal of Cell Science

Volumn 100, Issue 4, 1991, Pages 841-849

  Batra, Surinder K ^a   Kern, Horst F ^b   Worlock, Andrew J ^a   Metzgar, Richard S ^a   Hollingsworth, Michael A ^a,c  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Differentiation; Mucin; Transfection

Indexed keywords

MUCIN;

ARTICLE; CELL GRANULE; CELL LYSATE; CELL STRUCTURE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; GENE EXPRESSION; GENE FUSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOPEROXIDASE STAINING; PANCREAS; PRIORITY JOURNAL; ROUGH ENDOPLASMIC RETICULUM; TUMOR CELL LINE;

BLOTTING, NORTHERN; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CA-15-3 ANTIGEN; CELL DIFFERENTIATION; CYTOPLASM; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, STRUCTURAL, NEOPLASM; GOLGI APPARATUS; HUMAN; IMMUNOHISTOCHEMISTRY; MEMBRANE GLYCOPROTEINS; MICROSCOPY, ELECTRON; MUCINS; NEOPLASM PROTEINS; PANCREAS; SUPPORT, U.S. GOV'T, P.H.S.; TRANSCRIPTION, GENETIC; TRANSFECTION; TRANSLATION, GENETIC; TUMOR CELLS, CULTURED;

EID: 0026349593     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. BARND, D. L., LAN, M. S., METZGAR, R. S. AND FINN, O. J. (1989). Specific major histocompatibility complex-unrestricted recognition of tumor associated mucins by human cytotoxic T cells. Proc. natn. Acad. Sci. U.S.A. 86, 7159-7163.
2. BATRA, S. K., HOLLINGSWORTH, M. A. AND METZGAR, R. S. (1990). Carbonic anhydrase II and mucin gene expression in human pancreatic tumor cell lines of distinct states of differentiation. J. Cell Biol. 111, 219a.
3. BATRA, S. K., METZGAR, R. S. AND HOLLINGSWORTH, M. A. (1991). Molecular cloning and sequence analysis of the human ribosomal protein S16. J. biol. Chem. 266, 6830-6833.
4. BERGER, S. L. AND KIMMEL, A. R. (1987). Methods in Enzymology: Guide to Molecular Cloning Techniques, p. 812. New York: Academic Press, Inc.
5. BOROWITZ, M. J., TUCK, F. L., SINDELAR, W. F., FERNSTEN, P. D. AND METZGAR, R. S. (1984). Monoclonal antibodies against human pancreatic adenocarcinoma: Distribution of DU-PAN-2 antigen on glandular epithelia and adenocarcinomas. J. natn. Cancer Inst. 72, 999-1005.
6. BURCHELL, J., DURBIN, H. AND TAYLOR-PAPADIMITRIOU, J. (1983). Complexity of expression of antigenic determinants recognized by monoclonal antibodies HMFG-1 and HMFG-2 in malignant and mammary epithelial cells. J. Immun. 131, 508-513.
7. CHIRGWIN, J. M., PRZYBYLA, A. E., MACDONALD, R. J. AND RUTTER, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
8. GENDLER, S. J., LANCASTER, C. A., TAYLOR-PAPADIMITRIOU, J., DUHIG, T., PEAT, N., BURCHELL, J., PEMBERTON, L., LALANI, E. N. AND WILSON, D. (1990). Molecular cloning and expression of tumor-associated polymorphic epithelial mucin. J. biol. Chem. 265, 15 286-15 293.
9. GUNNING, P., LEAVITT, J., MUSCAT, G., NG, S. AND KEDES, L. (1987). A human β actin expression vector system directs high level accumulation of antisense transcripts. Proc. natn. Acad. Sci. U.S.A. 84, 4831-4835.
10. GUNNING, P., PONTE, P., OKAYAMA, H., ENGEL, J., BLAU, H. AND KEDES, L. (1983). Isolation and characterization of full length cDNA clones for human α-, β- and γ-actin mRNAs. Molec. cell. Biol. 3, 787-795.
11. HANISCH, F. G., UHLENBRUCK, G., PETER-KATALINIC, J., EGGE, H., DABROWSKI, J. AND DABROWSKI, U. (1989). Structures of neutral O-linked polylactosaminoglycans on human skim milk mucins. J. biol. Chem. 264, 872-883.
12. HEYDERMAN, E., STEELE, K. AND ORMEROD, M. G. (1979). A new antigen on the epithelial membrane: its immunoperoxidase localization in normal and neoplastic tissue. J. clin. Path. 32, 35-39.
13. KERN, H. F., ROHER, H. D., VON BULOW, M. AND KLOPPEL, G. (1987). Fine structure of three major grades of malignancy of human pancreatic adenocarcinoma. Pancreas 2, 2-13.
14. KHORRAMI, A., LAN, M. S., METZGAR, R. S. AND KAUFMAN, B. (1989). Characteristics of a sulfated human pancreatic adenocarcinoma mucin glycoprotein. Glycoconjugate J. 6, 428a.
15. KIM, Y. W., KERN, H. F., MULLINS, T. D., KORIWCHAK, M. J. AND METZGAR, R. S. (1989). Characterization of clones of a human pancreatic adenocarcinoma cell line representing different stages of differentiation. Pancreas 4, 353-362.
16. LAN, M. S., BATRA, S. K., QI, W. N., METZGAR, R. S. AND HOLLINGSWORTH, M. A. (1990). Cloning and sequencing of a human pancreatic tumor mucin cDNA. J. biol. Chem. 265, 15 294-15 299.
17. LAN, M. S., KHORRAMI, A., KAUFMAN, B. AND METZGAR, R. S. (1987). Molecular characterization of mucin-type antigen associated with human pancreatic cancer. J. biol. Chem. 262, 12863-12870.
18. LIEBER, M., MAZZETTA, J., NELSON-REES, W., KAPLAN, M. AND TORADO, G. (1975). Establishment of a continuous tumor cell line (Panc 1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15, 741-747.
19. MANIATIS, T., FRITSCH, E. F. AND SAMBROOK, J. (1982). Molecular Cloning: A Laboratory Manual, p. 545. New York: Cold Spring Harbor Laboratory.
20. METZGAR, R. S., RODRIGUEZ, N., FINN, O. J., LAN, M. S., DAASCH, V. N., FERNSTEN, P. D., MEYERS, W. C., SINDELAR, W. F., SANDLER, R. S. AND SEIGLER, H. F. (1984). Detection of a pancreatic cancer associated antigen (DU-PAN-2) in serum and ascites of patients with adenocarcinoma. Proc. natn. Acad. Sci. U.S.A. 81, 5242-5246.
21. MULLINS, T. D., KERN, H. F. AND METZGAR, R. S. (1991). Ultrastructural differentiation of sodium butyrate treated human pancreatic adenocarcinoma cell lines. Pancreas 6, 578-587.
22. PARRY, G., BECK, J. C., MOSS, L., BARTLEY, J. AND OJAKIAN, G. K. (1990). Determination of apical membrane polarity in mammary epithelial cell cultures: The role of cell-cell, cell-substratum, and membrane-cytoskeleton interactions. Expl Cell Res. 188, 302-311.
23. RITTENHOUSE, H. G., MANDERINO, G. L. AND HASS, G. M. (1985). Mucin type glycoproteins as tumor markers. Lab. Med. 16, 556-560.
24. SLOANE, J. P., ORMEROD, M. G., IMRIE, S. F. AND COOMBES, R. C. (1980). The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections. Br. J. Cancer 42, 392-398.
25. SLOANE, J. P. AND ORMEROD, M. G. (1981). Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer 47, 1786-1795.
26. WEIBEL, E. R. (1979). Stereological Methods. I. Practical Methods for Biological Morphometry. London: Academic Press, Inc.
27. ZOTTER, S., HAGEMAN, P. C., LOSSNITZER, A., MOOI, W. J. AND HILGERS, J. (1988). Tissue and tumor distribution of human polymorphic epithelial mucin. Cancer Rev. 11-12, 55-101.