Production and Characterization of Monoclonal Antibodies That Localize Human Thymidylate Synthase in the Cytoplasm of Human Cells and Tissue

Cancer Research

Volumn 51, Issue 24, 1991, Pages 6668-6676

  Johnston, Patrick G ^a,b   Liang, Chi Ming ^a   Henry, Sally ^a   Chabner, Bruce A ^a   Allegra, Carmen J ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUOROURACIL; MONOCLONAL ANTIBODY; THYMIDYLATE SYNTHASE;

ARTICLE; COLON CARCINOMA; CONTROLLED STUDY; CYTOPLASM; DRUG RESISTANCE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BLOTTING, WESTERN; CARCINOMA; COLONIC NEOPLASMS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; FLUORODEOXYURIDYLATE; HUMAN; IMMUNOENZYME TECHNIQUES; PRECIPITIN TESTS; THYMIDYLATE SYNTHASE;

EID: 0026335113     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. Danenberg, P. V. Thymidylate synthase-a target enzyme in cancer chemotherapy. Biochim. Biophys. Acta, 473: 73-92, 1977.
2. Santi, D. V., McHenry, C. S., and Sommer, M. Mechanisms of interaction of thymidylate synthase with 5-fluorodeoxyuridylate. Biochemistry, 13:471-480, 1974.
3. Moertel, C. G. Current concepts in cancer: chemotherapy of gastrointestinal cancer. N. Engl. J. Med., 299: 1049-1052, 1978.
4. Santi, D. V., and Danenberg, P. V. Folates in pyrimidine nucleotide biosynthesis. In: R. L. Blakley and S. J. Benkovic (eds.), Folates and Pteridines, pp. 345-398. New York: Wiley, 1984.
5. Heidelberger, C. Fluorinated pyrimidines and their nucleosides. In: A. C. Sartorelli and D. G. Johns (eds.), Handbook of Experimental Pharmacology, Vol. 38, pp. 193-231. New York: Springer Verlag, 1975.
6. Hardy, L. W., Finer-Moore, J., Montfort, W., Jones, M., Santi, D. V., and Stroud, R. M. Atomic structure of thymidylate synthase: target for rational drug design. Science (Washington DC), 235:448-453, 1987.
7. Chu, F. K., Maley, F., and Belfort, M. Intervening sequence in the thymidylate synthase gene of bacteriophage T4. Proc. Natl. Acad. Sci. USA, 81: 3049-3053, 1984.
8. Belfort, M., Maley, G., Lane, J. P., and Maley, F. Primary structure of Escherichia coli thy A gene and its thymidylate synthase product. Proc. Natl. Acad. Sci. USA, 80: 4914-4918, 1983.
9. Beverley, S. M., Ellenberger, T. E., and Cordingly, J. S. Primary structure of the gene encoding the bifunctional dihydrofolate reduction-thymidylate synthase of leishmania major. Proc. Natl. Acad. Sci. USA, 83: 2584-2589, 1986.
10. Conrad, A. H., and Ruddle, F. H. Regulation of thymidylate synthase activity in cultured mammalian cells. J. Cell Sci., 10: 471-486, 1972.
11. Navalgund, L. G., Rossann, C., Muench, A. J., and Johnson, L. F. Cell cycle regulation of thymidylate synthase gene expression in culture mouse fibroblasts. J. Biol. Chem., 255:7386-7390, 1980.
12. Johnson, L. F. Expression of dihydrofolate reductase and thymidylate synthase in mammalian cells. In: G. S. Stein and J. L. Stein (eds.), Recombinant DNA and Cell Proliferation, pp. 25-47. New York: Academic Press, 1984.
13. Jenh, C. H., Rao, L. G., and Johnson, L. F. Regulation of thymidylate synthase enzyme synthesis in 5-fluorodeoxyuridine resistant mouse fibroblasts during the transition from the resting to growing state. J. Cell. Physiol., 122: 149-154, 1985.
14. Berger, S. H., Chung-Her, J., Johnson, L. F., and Berger, F. G. Thymidylate synthase overproduction and gene amplification in fluorodeoxyuridine-re-sistant human cells. Mol. Pharmacol., 28:461-467, 1987.
15. Clark, J. L., Berger, S. L., Mittelman, A., and Berger, F. G. Thymidylate synthase gene amplification in a colon tumor resistant to fluoropyrimidine chemotherapy. Cancer Treat. Rep., 71: 261-265,1987.
16. Spears, C. P., Antranik, A. H., Moran, R. G., Heidelberger, C., and Corbett, T. H. In vivo kinetics of thymidylate synthase inhibition in 5-fluorouracil sensitive and resistant murine colon adenocarcinomas. Cancer Res., 42:450-456, 1982.
17. Washtein, W. L. Increased levels of thymidylate synthase in cells exposed to 5-fluorouracil. Mol. Pharmacol., 25: 171-177, 1984.
18. Chu, E., Zinn, S., Boarman, D., and Allegra, C. J. The interaction of 7-interferon and 5-fluorouracil in the H630 human colon carcinoma cell line. Cancer Res., 50: 5834-5840, 1990.
19. Keyomarsi, K., and Moran, R. G. Mechanism of cytotoxic synergism of fluoropyrimidines and folinic acid in mouse leukemia cells. J. Biol. Chem., 263: 14402-14409, 1988.
20. Spears, C. P., Gustavsson, B. G., Berne, M., Frosling, R., Bernstein, L., and Hayes, A. A. Mechanisms of innate resistance to thymidylate synthase inhibition after 5-fluorouracil. Cancer Res., 48:5894-5900, 1988.
21. Swain, S. M., Lippman, M. E., Egan, E. F., Drake, J. C., Steinberg, S. M., and Allegra, C. J. Fluorouracil and high-dose leucovorin in previously treated patients with metastatic breast cancer. J. Clin. Oncol., 7: 890-896, 1989.
22. Scanlon, K. J., and Kashani-Sabet, M. Elevated expression of thymidylate synthase cycle genes in cisplatin-resistant human ovarian carcinoma. Proc. Natl. Acad. Sci. USA, 85:650-653, 1988.
23. Lockshin, A., and Danenberg, P. V. Biochemical factors affecting the tightness of 5-fluorodeoxyuridylate binding to human thymidylate synthase. Biochem. Pharmacol., 30: 247-257,1981.
24. Lockshin, A., and Danenberg, P. V. Thymidylate synthase and 2-deoxyuri-dylate form a tight complex in the presence of pteroyltriglutamate. J. Biol. Chem., 254: 12285-12288, 1979.
25. Davisson, V. J., Shirawarapon, W., and Santi, D. V. Expression of human thymidylate synthase in Escherichia coli. J. Biol. Chem., 264: 9145-9148, 1989.
26. Galfre, G., Howe, S. C., Milstein, C., Butcher, G. W., and Howard, J. C. Antibodies to msgor histocompatibility antigens produced by hybrid cell lines. Nature (Lond.), 266:550-553, 1977.
27. Liang, C-M., Herren, S., Said, A., and Jost, T. Studies of antigenic epitopes recognize the monoclonal antibodies to recombinant interferon-7. Biochem. Biophys. Res. Commun., 128: 171-178, 1985.
28. FPLC for Monoclonal Antibody Purification (Pharmacia brochure, Piscata-way, NJ, 1990).
29. Ouchterlony, O., and Nilsson, L. A. Immunodiffusion and Immunoelectrophoresis. In: D. M. Weir (ed.), Handbook of Experimental Immunology, pp. 655-707. Oxford and Edinburgh: Blackwell, 1986.
30. Thorell, J. L., and Johnansson, B. G. Enzymatic iodination of polypeptides with, 25I to high specific activity. Biochim. Biophys. Acta, 251: 363-369, 1971.
31. Nakane, P. K., and Kawaoi, A. Peroxidase-labeled antibody: a new method of conjugation. J. His toe hem. Cytochem., 22: 1084-1091,1974.
32. Heyman, B., Holmquist, G., Borwell, P., and Heyman, U. An enzyme linked immunosorbent assay for measuring anti-sheep erythrocyte antibodies. J. Immunol. Methods, 68: 193-204, 1984.
33. Scatchard, G. The attraction of proteins for small molecules and ions. Ann. N.Y. Acad. Sci., 51:660-672, 1949.
34. Park, J. G., Oie, H. K., Sugarbaker, P. H., Henslee, J. G., Chen, T. R., Johnson, B. E., and Gazdar, A. Characterization of cell lines established for human colorectal carcinoma. Cancer Res., 47:6710-6718, 1987.
35. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 227: 680-685, 1970.
36. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 72: 248-254, 1976.
37. Hsu, S. M., Raine, L., and Fanger, H. Use of aviden biotin peroxidase complex (ABC) in immunoperoxidase techniques. J. Histochem. Cytochem., 29:577-585, 1981.
38. Hsu, S. M., Raine, L., and Fanger, H. A comparative study of the peroxidase-antiperoxidase method and an avidin biotin complex method for studying polypeptide hormone with radioimmunoassay antibodies. Am. J. Clin. Pathol., 75: 734-738, 1981.
39. Jastreboff, M. M., Todd, M. B. Malech, H. L., and Bertino, J. R. Isolation and functional effects of monoclonal antibodies binding to thymidylate synthase. Biochemistry, 24: 587-592, 1985.
40. Lipkin, M., Blattner, W., Gardner, E., Burt, R., Lynch, H., Deschner, E., Winawer, S., and Fraumeni, J. F. Classification and risk assessment of individuals with familial polyposis, Gardner's syndrome, and familial nonpolyposis colon cancer from [3H]thymidine labeling patterns in colonic epithelial cells. Cancer Res., 44:4201-4207,1984.
41. Meyer, J. S., and Hixon, B. Advanced stage and early relapse of breast carcinomas associated with high thymidine labeling indices. Cancer Res., 39: 4042-4047, 1979.