Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates

New England Journal of Medicine

Volumn 326, Issue 3, 1992, Pages 161-166

  Weber, Rainer ^a   Bryan, Ralph T ^a   Visvesvara, Govinda S ^a   Owen, Robert L ^b   Wilcox, C Mel ^c   Gorelkin, Leo ^d  

^a Division of Parasitic Diseases   (United States)

^b NATL CENTER FOR INFECTIOUS DISEASES   (United States)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d NONE

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHRONIC DIARRHEA; DUODENUM BIOPSY; ENDOSCOPY; FECES ANALYSIS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTESTINE INFECTION; MAJOR CLINICAL STUDY; MICROSCOPY; MICROSPORUM; PREDICTION; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY;

ANIMAL; CHRONIC DISEASE; DIARRHEA; DUODENUM; FECES; HIV INFECTIONS; MICROSCOPY; MICROSPORIDA; MICROSPORIDIOSIS; PROSPECTIVE STUDIES; SPORES; STAINING AND LABELING; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0026334776     PISSN: 00284793     EISSN: 15334406     Source Type: Journal    
DOI: 10.1056/NEJM199201163260304     Document Type: Article

References (39)

1. Canning E.U., Lom J., Dykova I. The microsporidia of vertebrates. San Diego, Calif.: Academic Press, 1986.
2. Bryan R.T. Microsporidia. In: Mandell GL, Douglas RG, Bennett JE, eds. Principles and practice of infectious diseases. 3rd ed. New York: Churchill Livingstone, 1990: 2130-4.
3. Bryan R.T., Cali A., Owen R.L., Spencer H.C. Microsporidia: opportunistic pathogens in patients with AIDS. In: Sun T, ed. Progress in clinical parasitology. Vol. 2. Philadelphia: Field & Wood, 1991: 1-26.
4. Canning E.U., Hollister W.S. Enterocytozoon bieneusi (Microspora): prevalence and pathogenicity in AIDS patients. Trans R Soc Trop Med Hyg 1990; 84:181-186
5. Matsubayashi H., Koike T., Mikata T., Takei H., Hagiwara S. A case of Encephalitozoon-like body infection in man. Arch Pathol 1959; 67:181-187
6. Bergquist N.R., Stintzing G., Smedman L., Waller T., Andersson T. Diagnosis of encephalitozoonosis in man by serological tests. BMJ 1984; 288:902.
7. Margileth A.M., Strano A.J., Chandra R., Neafie R., Blum M., McCully R.M. Disseminated nosematosis in an immunologically compromised infant. Arch Pathol 1973; 95:145-150
8. Ledford D.K., Overman M.D., Gonzalo A., Cali A., Mester S.W., Lockey R.F. Microsporidiosis myositis in a patient with acquired immunodeficiency syndrome. Ann Intern Med 1985; 102:628-630
9. Ashton N., Wirasinha P.A. Encephalitozoonosis (nosematosis) of the cornea. Br J Ophthalmol 1973; 57:669-674
10. Pinnolis M., Egbert P.R., Font R.L., Winter F.C. Nosematosis of the cornea. Arch Ophthalmol 1981; 99:1044-1047
11. Davis R.M., Font R.L., Keisler M.S., Shadduck J.A. Corneal microsporidiosis: a case report including ultrastructural observations. Ophthalmology 1990; 97:953-957
12. Cali A., Meisler D., Lowder C.Y., et al. Corneal microsporidiosis, characterization and identification. J Protozool 1991; 38:215-217
13. Modigliani R., Bories C., Le Charpentier Y., et al. Diarrhoea and malabsorption in acquired immune deficiency syndrome: a study of four cases with special emphasis on opportunistic protozoan infestations. Gut 1985; 26:179-187
14. Dobbins W.O. III, Weinstein W.M. Electron microscopy of the intestine and rectum in acquired immunodeficiency syndrome. Gastroenterology 1985; 88:738-749
15. Shadduck J.A. Human microsporidiosis and AIDS. Rev Infect Dis 1989; 11:203-207
16. Terada S., Reddy K.R., Jeffers L.J., Cali A., Schiff E.R. Microsporidian hepatitis in the acquired immunodeficiency syndrome. Ann Intern Med 1987; 107:61-62
17. Zender H.O., Arrigoni E., Eckert J., Kapanci Y. A case of Encephalitozoon cuniculi peritonitis in a patient with AIDS. Am J Clin Pathol 1989; 92:352-356
18. Microsporidian keratoconjunctivitis in patients with AIDS. MMWR 1990; 39:188-189
19. Didier E.S., Didier P.J., Friedberg D.N., et al. Isolation and characterization of a new human microsporidian, Encephalitozoon hellem (n. sp.), from three AIDS patients with keratoconjunctivitis. J Infect Dis 1991; 163:617-621
20. Friedberg D.N., Stenson S.M., Orenstein J.M., Tierno P.M., Charles N.C. Microsporidial keratoconjunctivitis in acquired immunodeficiency syndrome. Arch Ophthalmol 1990; 108:504-508
21. Curry A., McWilliam L.J., Haboubi N.Y., Mandal B.K. Microsporidiosis in a British patient with AIDS. J Clin Pathol 1988; 41:477-478
22. Cali A., Owen R.L. Intracellular development of Enterocytozoon, a unique microsporidian found in the intestine of AIDS patients. J Protozool 1990; 37:145-155
23. Gourley W.K., Swedo J.L. Intestinal infection by microsporidia Enterocytozoon bieneusi of patients with AIDS: an ultra-structural study of the use of human mitochondria by a protozoa. Lab Invest 1988; 58:35A.
24. Lucas S.B., Papadaki L., Conlon C., Sewankambo N., Goodgame R., Serwadda D. Diagnosis of intestinal microsporidiosis in patients with AIDS. J Clin Pathol 1989; 42:885-890
25. Orenstein J.M., Chiang J., Steinberg W., Smith P.D., Rotterdam H., Kotler D.P. Intestinal microsporidiosis as a cause of diarrhea in human immunodeficiency virus–infected patients: a report of 20 cases. Hum Pathol 1990:21:475-481
26. van Gool T., Hollister W.S., Schattenkerk W.E., et al. Diagnosis of Enterocytozoon bieneusi microsporidiosis in AIDS patients by recovery of spores from faeces. Lancet 1990; 336:697-698
27. Paik G., Suggs T.M. Reagents, stains, and miscellaneous test procedures. In: Lennette EH, Spaulding EH, Truand JP, eds. Manual of clinical microbiology. 2nd ed. Washington, D.C.: American Society for Microbiology, 1974: 930-50.
28. Ritchie L.S. An ether sedimentation technique for routine stool examinations. Bull U S Army Med Dept 1948; 8:326.
29. Young K.H., Bullock S.L., Melvin D.M., Spruill C.L. Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol 1979; 10:852-853
30. Sheather A.L. The detection of intestinal protozoa and mange parasites by a flotation technique. J Comp Pathol 1923; 36:266-275
31. Jouvenaz D.P. Percoll: an effective medium for cleaning microsporidian spores. J Invertebr Pathol 1981; 37:319.
32. Rijpstra AC, Canning E.U., Van Ketel R.J., Eeftinck Schattenkerk J.K.M., Laarman J.J. Use of light microscopy to diagnose small-intestinal microsporidiosis in patients with AIDS. J Infect Dis 1988; 157:827-831
33. Ullrich R., Zeitz M., Heise W., L#x0027;age M., Höffken G., Riecken E.O. Small intestinal structure and function in patients infected with human immunodeficiency virus (HIV): evidence for HIV-induced enteropathy. Ann Intern Med 1989; 111:15-21.
34. Erratum, Ann Intern Med 1989; 111:954.
35. Conlon C.P., Pinching A.J., Perera C.U., Moody A., Luo N.P., Lucas S.B. HIV-related enteropathy in Zambia: a clinical, microbiological, and histological study. Am J Trop Med Hyg 1990; 42:83-88
36. Kotler D.P., Francisco A., Clayton F., Scholes J.V., Orenstein J.M. Small intestinal injury and parasitic diseases in AIDS. Ann Intern Med 1990; 113:444-449
37. Greenson J.K., Belitsos P.C., Yardley J.H., Bartlett J.G. AIDS enteropathy: occult enteric infections and duodenal mucosal alterations in chronic diarrhea. Ann Intern Med 1991; 114:366-372
38. Eeftinck Schattenkerk J.K.M., van Gool T., van Ketel R.J., et al. Clinical significance of small-intestinal microsporidiosis in HIV-1-infected individuals. Lancet 1991; 337:895-898
39. Orenstein J.M., Zierdt W., Zierdt C., Kotler D.P. Identification of spores of Enterocytozoon bieneusi in stool and duodenal fluid from AIDS patients. Lancet 1990; 336:1127-1128