-
1
-
-
0024655878
-
Enzymatic catalysis in monophasic organic solvents
-
Dordick, J. S, 1989. Enzymatic catalysis in monophasic organic solvents. Enz. Microb. Technol. 11: 194-211.
-
(1989)
Enz. Microb. Technol
, vol.11
, pp. 194-211
-
-
Dordick, J.S.1
-
2
-
-
0022724718
-
Enzymes that work in organic solvents
-
June
-
Klibanov, A. M. 1986. Enzymes that work in organic solvents. Chemtech. June: 354-359.
-
(1986)
Chemtech
, pp. 354-359
-
-
Klibanov, A.M.1
-
3
-
-
0025485121
-
Enzyme engineering for non-aqueous solvents
-
Arnold, F. H. 1990. Enzyme engineering for non-aqueous solvents. Trends Biotechnol. 8: 244-249.
-
(1990)
Trends Biotechnol
, vol.8
, pp. 244-249
-
-
Arnold, F.H.1
-
4
-
-
0000903544
-
Surface charge substitutions increase the stability of α-lytic protease in organic solvents
-
Martinez, P. and Arnold, F. H. 1991. Surface charge substitutions increase the stability of α-lytic protease in organic solvents. J. Am. Chem. Soc. 113: 6336-6337.
-
(1991)
J. Am. Chem. Soc
, vol.113
, pp. 6336-6337
-
-
Martinez, P.1
Arnold, F.H.2
-
5
-
-
0026124848
-
Enzyme engineering for nonaqueous solvents II
-
Chen, K., Robinson, A. C., Van Dam, M. E, Martinez, P., Economou, C. and Arnold, F. H. 1991. Enzyme engineering for nonaqueous solvents II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media. Biotechnol. Prog. 7: 125-129.
-
(1991)
Additive effects of mutations on the stability and activity of subtilisin E in polar organic media. Biotechnol. Prog
, vol.7
, pp. 125-129
-
-
Chen, K.1
Robinson, A.C.2
Van Dam, M.E.3
Martinez, P.4
Economou, C.5
Arnold, F.H.6
-
6
-
-
85168596183
-
Stabilization of subtilisin E in organic solvents by site-directed mutagenesis
-
8c Bioeng. In press
-
Martinez, P., Van Dam, M. E., Robinson, A, C., Chen, K. and Arnold, F. H. 1991. Stabilization of subtilisin E in organic solvents by site-directed mutagenesis. Biotechnol. 8c Bioeng. In press.
-
(1991)
Biotechnol
-
-
Martinez, P.1
Van Dam, M.E.2
Robinson, A.C.3
Chen, K.4
Arnold, F.H.5
-
7
-
-
33845279863
-
A search for peptide ligase: Cosolvent-mediated conversion of proteases to esterases for irreversible synthesis of peptides
-
Barbas, C. F., III, Matos, J. R., West, J. B., Wong, C. H. 1988. A search for peptide ligase: cosolvent-mediated conversion of proteases to esterases for irreversible synthesis of peptides. J. Am. Chem. Soc. 110: 5162-5166.
-
(1988)
J. Am. Chem. Soc
, vol.110
, pp. 5162-5166
-
-
Barbas, C.F.1
Matos, J.R.2
West, J.B.3
Wong, C.H.4
-
8
-
-
33845280481
-
Protease-catalyzed reeioselective esterification of sugars and related compounds in anhydrous dimethylformamide
-
Riva, S., Chopineau, J., Kieboom, A. P. G. and Klibanov, A. M, 1988. Protease-catalyzed reeioselective esterification of sugars and related compounds in anhydrous dimethylformamide. J. Am. Chem. Soc. 110: 584-589.
-
(1988)
J. Am. Chem. Soc
, vol.110
, pp. 584-589
-
-
Riva, S.1
Chopineau, J.2
Kieboom, A.P.G.3
Klibanov, A.M.4
-
9
-
-
0039234462
-
Subtilisins: Primary structure, chemical and physical properties
-
P.D. Boyer (Ed.). Academic Press, NY
-
Markland, Jr., F. S. and Smith, E. L. 1971. Subtilisins: Primary structure, chemical and physical properties, p. 561-608. In: The Enzymes. 3rd ed., Vol. III. P.D. Boyer (Ed.). Academic Press, NY.
-
(1971)
The Enzymes. 3rd ed
, vol.3
, pp. 561-608
-
-
Markland, F.S.1
Smith, E.L.2
-
10
-
-
77956896878
-
Subtilisin: X-ray structure
-
Kraut, J. 1971. Subtilisin: X-ray structure, p. 547-560. In: The Enzymes. 3rd ed., Vol. III.
-
(1971)
The Enzymes. 3rd ed
, vol.3
, pp. 547-560
-
-
Kraut, J.1
-
11
-
-
0023783073
-
Subtilisin-an enzyme designed to be engineered
-
Wells, J. A. and Estell, D. A. 1988. Subtilisin-an enzyme designed to be engineered. Trends Biochem. Sci. 13: 291-297.
-
(1988)
Trends Biochem. Sci
, vol.13
, pp. 291-297
-
-
Wells, J.A.1
Estell, D.A.2
-
12
-
-
0021337529
-
Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation
-
Stahl, M. L. and Ferrari, E. 1984. Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation. J. Bacteriol. 158: 411-418.
-
(1984)
J. Bacteriol
, vol.158
, pp. 411-418
-
-
Stahl, M.L.1
Ferrari, E.2
-
13
-
-
0002694056
-
A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
-
Leung, D. W., Chen, E. and Goeddel, D. V. 1989. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique 1: 11-15.
-
(1989)
Technique
, vol.1
, pp. 11-15
-
-
Leung, D.W.1
Chen, E.2
Goeddel, D.V.3
-
14
-
-
85168628507
-
Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E
-
8c Bioeng. In press
-
Economou, C., Chen, K. and Arnold, F. H. 1991. Random mutagenesis to enhance the activity of subtilisin in organic solvents: Characterization of Q103R subtilisin E. Biotechnol. 8c Bioeng. In press.
-
(1991)
Biotechnol
-
-
Economou, C.1
Chen, K.2
Arnold, F.H.3
-
15
-
-
0015497445
-
Subtilisin; a stereochemical mechanism involving transition-state stabilization
-
Robertus, J. D., Kraut, J., Alden, R. A. and Birktoft, J.J. 1972. Subtilisin; a stereochemical mechanism involving transition-state stabilization. Biochemistry 23: 4293-4303.
-
(1972)
Biochemistry
, vol.23
, pp. 4293-4303
-
-
Robertus, J.D.1
Kraut, J.2
Alden, R.A.3
Birktoft, J.J.4
-
16
-
-
0024522967
-
Thermitase, a thermostable subtilisin: Comparison of predicted and experimental structures and the molecular cause of thermostability
-
Frommel, C. and Sander, C. 1989. Thermitase, a thermostable subtilisin: comparison of predicted and experimental structures and the molecular cause of thermostability. Proteins: Struct. Funct. Genet. 5: 22-37.
-
(1989)
Proteins: Struct. Funct. Genet
, vol.5
, pp. 22-37
-
-
Frommel, C.1
Sander, C.2
-
17
-
-
0022989294
-
Proteases of enhanced stability: Characterization of a thermostable variant of subtilisin
-
Bryan, P. N., Rollence, M. L., Pantoliano, M. W., Wood, J., Finzel, B.C., Gilliland, G. L., Floward, A. J. and Poulos, T. L. 1986. Proteases of enhanced stability: characterization of a thermostable variant of subtilisin. Proteins: struct., Funct., Genet. 1: 326-334.
-
(1986)
Proteins: Struct., Funct., Genet
, vol.1
, pp. 326-334
-
-
Bryan, P.N.1
Rollence, M.L.2
Pantoliano, M.W.3
Wood, J.4
Finzel, B.C.5
Gilliland, G.L.6
Floward, A.J.7
Poulos, T.L.8
-
19
-
-
0023850178
-
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
-
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G. T., Mullis, K. B. and Erlich, H. A. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491.
-
(1988)
Science
, vol.239
, pp. 487-491
-
-
Saiki, R.K.1
Gelfand, D.H.2
Stoffel, S.3
Scharf, S.J.4
Higuchi, R.5
Horn, G.T.6
Mullis, K.B.7
Erlich, H.A.8
-
20
-
-
0015222721
-
Fate of transforming DNA following uptake by competent Bacillus subtilis. J
-
Dubnau, D. and Davidoff-Abelson, R. 1971. Fate of transforming DNA following uptake by competent Bacillus subtilis. J. Mol. Biol. 56: 209-221.
-
(1971)
Mol. Biol
, vol.56
, pp. 209-221
-
-
Dubnau, D.1
Davidoff-Abelson, R.2
-
21
-
-
0017847690
-
Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J
-
Gryczan, T. J., Contente, S. and Dubnau, D, 1978. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329.
-
(1978)
Bacteriol
, vol.134
, pp. 318-329
-
-
Gryczan, T.J.1
Contente, S.2
Dubnau, D.3
-
22
-
-
0015239543
-
The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J
-
Leighton, T. J. and Doi, R. H. 1971. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J. Biol. Chem. 246: 3189-3195.
-
(1971)
Biol. Chem
, vol.246
, pp. 3189-3195
-
-
Leighton, T.J.1
Doi, R.H.2
-
23
-
-
0023634094
-
Production of single-stranded plasmid DNA
-
Vieira, J. and Messing, J. 1987. Production of single-stranded plasmid DNA. Methods Enzymol. 153: 3-11.
-
(1987)
Methods Enzymol
, vol.153
, pp. 3-11
-
-
Vieira, J.1
Messing, J.2
-
25
-
-
0023371227
-
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase
-
Tabor, S. and Richardson, C. C. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84: 4767-4771.
-
(1987)
Proc. Natl. Acad. Sci. USA
, vol.84
, pp. 4767-4771
-
-
Tabor, S.1
Richardson, C.C.2
-
26
-
-
0021830125
-
Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation
-
Estell, D. A., Graycar, T. P. and Wells, J. A. 1985. Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation. J. Biol. Chem. 260: 6518-6521.
-
(1985)
J. Biol. Chem
, vol.260
, pp. 6518-6521
-
-
Estell, D.A.1
Graycar, T.P.2
Wells, J.A.3
-
27
-
-
0014949207
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
-
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
-
(1970)
Nature
, vol.227
, pp. 680-685
-
-
Laemmli, U.K.1
|