A novel system for large-scale sequencing of cDNA by PCR amplification

Mitochondrial DNA

Volumn 2, Issue 3, 1991, Pages 137-144

  Okubo, Kousaku ^a   Hori, Naohiro ^a   Matoba, Ryo ^a   Niiyama, Toshiyuki ^a   Matsubara, Kenichi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

3' UTR; CDNA library; Hep G2; Large scale sequencing; PCR artifact; PCR direct sequencing

Indexed keywords

DNA;

ARTICLE; CELL CULTURE; GENE LIBRARY; HUMAN; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; DNA; GENE LIBRARY; HUMAN; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; TUMOR CELLS, CULTURED;

EID: 0026301763     PISSN: 19401744     EISSN: None     Source Type: Journal    
DOI: 10.3109/10425179109039684     Document Type: Article

References (19)

1. Controlled synthesis of HBsAg in a differentiated human liver carcinoma derived cell line
2. Purification of biological active globin messenger RNA by chromatography on oligo thymidic acid-cellulose
3. Full-length cDNA cloning: Vector Primed, DNA synthesis
4. Direct clone characterization from plaques and colonies by polymerase chain reaction
5. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
6. High efficiency transformation of Escherichia coli, with plasmids
7. Production of singlestranded DNA by asymmetric PCR
8. DNA recombination during PCR
9. Construction of improved M13 vectors using oligo deoxynucleotide directed mutagenesis
10. High-efficiency cloning of full-length cDNA
11. High efficiency cloning of full-length cDNA
12. Enzymatic amplification of β-globulin genomic sequences and restriction site analysis/or diagnosis of sickle cell anemia
13. Extraction and purification of RNA
14. DNA sequencing with chain-terminating inhibitors
15. Rapid polymerase chain reaction based analysis of DNA fragments cloned in lac Z vectors
16. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors