Leishmania donovani: Long-term culture of axenic amastigotes at 37 °C

Experimental Parasitology

Volumn 73, Issue 3, 1991, Pages 326-334

  Doyle, Patricia S ^a   Engel, Juan C ^a   Pimenta, Paulo F P ^b   da Silva, Pedro Pinto ^b   Dwyer, Dennis M ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Axenic amastigotes; Cell transformation; Growth kinetics; Leishmania donovani; Parasitic protozoa

Indexed keywords

ACID PHOSPHATASE;

ANIMAL; ARTICLE; CELL CYCLE; CELL MEMBRANE; CULTURE MEDIUM; CYTOLOGY; ELECTRON MICROSCOPY; ENZYMOLOGY; FLAGELLUM; FREEZE FRACTURE; GROWTH, DEVELOPMENT AND AGING; KINETICS; LEISHMANIA DONOVANI; METABOLISM; TEMPERATURE; ULTRASTRUCTURE;

ACID PHOSPHATASE; ANIMAL; CELL CYCLE; CELL MEMBRANE; CULTURE MEDIA; FLAGELLA; FREEZE FRACTURING; KINETICS; LEISHMANIA DONOVANI; MICROSCOPY, ELECTRON; TEMPERATURE;

EID: 0026245629     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/0014-4894(91)90104-5     Document Type: Article

References (28)

1. Fracture-flip: New resolution images of cell surfaces after stabilization of freeze-fractured membranes
2. Fusion of host cell secondary lysosomes with parasitophorous vacuoles of Leishmania mexicana-infected macrophages
3. Biosynthesis and secretion of acid phosphatase by Leishmania donovani promastigotes
4. Leishmania donovani: Generation of monospecific antibody reagents to soluble acid phosphatase
5. Multiplication of a human parasite (Leishmania donovani) in phagolysosomes of hamster macrophages in vitro
6. In vitro reversible transformation of Leishmania brasiliensis panamensis between promastigotes and ellipsoidal forms
7. Trypanosoma cruzi: Cell biological behaviour of epimastigotes and amastigotes in axenic culture
8. Trypanosoma cruzi cells undergo an alteration in protein N-glycosylation upon differentiation
9. I. Extracellular cultivation and morphological character ization of amastigote-like forms of Leishmania panamensis and Leishmania brasiliensis
10. Extracellular amastigote-like forms of Leishmania panamensis and L. brasiliensis. II. Stage- and species-specific monoclonal antibodies
11. Effects of promastigotes growth phase, frequency of subculture, and host age on promastigotes-initiated infections in Leishmania donovani in the golden hamster
12. Biology of Leishmania in phlebotomine sand flies
13. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4
14. Effect of temperature elevation on mRNA and protein synthesis in Leishmania mexicana amanzonensis
15. Leishmania mexicana: Serial cultivation of intracellular stages in a cell-free medium
16. Leishmania mexicana pifanoi: Analysis of antigenic relationships between promastigotes and amastigotes by gel diffusion, immunoelectrophoresis, and immunoprecipitation
17. Cell surface nanoanatomy of Leishmania major as revealed by fracture-flip. A surface meshwork of 44 nm fusiform filaments identifies infective developmental stage promastigotes
18. The cell surface of T. cruzi: A fracture-flip, replica-staining label-fracture
19. The comparative fine structure and surface glycoconjugate expression of three life stages of Leishmania major
20. Identification of an infective stage of Leishmania promastigotes
21. A two-dimensional gel electrophoresis analysis of the conversion of Leishmania brasiliensis panamensis promastigotes to amastigotes forms
22. Leishmania brasiliensis panamensis: Increased infectivity resulting from heat shock
23. Morphology of Leishmania brasiliensis: Change during reversible heat-induced transformation from promastigotes to an ellipsoidal form
24. In vitro growth of parasites
25. The influence of temperature and serum deprivation on the synthesis of heat-shock proteins and alpha and beta tubulin in promastigotes of Leishmania major
26. Heat shock genes: Regulatory role for differentiation in parasitic protozoa
27. Effects of phorbol ester on Leishmania mexicana amazonensis: An ultrastructural and cytochemistry study
28. Species and stage specificity of Leishmania donovani antigens