The 16s/23s ribosomal spacer region as a target for DNA probes to identify eubacteria

Genome Research

Volumn 1, Issue 1, 1991, Pages 51-56

  Barry, Tom ^a   Colleran, Gerard ^a   Glennon, Maura ^a   Dunican, L K ^a   Gannon, Frank ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; RNA 16S; RNA 23S;

ARTICLE; BACTERIUM; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; DNA PROBE; GENETIC PROCEDURES; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE;

BACTERIA; BASE SEQUENCE; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS; DNA PROBES; EVALUATION STUDIES; MOLECULAR PROBE TECHNIQUES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECIES SPECIFICITY; SUPPORT, NON-U.S. GOV'T;

EID: 0026207421     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.1.1.51     Document Type: Article

References (12)

1. Macario, A.J.L. and E.C. de Macario. 1990. Gene probes for bacteria. Academic Press,
2. Barry T., R. Powell, and F. Cannon. 1990. A general method to generate DNA probes for microorganisms. BioTechnology 8: 233-236.
3. Saiki, R.K., D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis, and H.A. Erlich. 1988. Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491
4. Srivastava, A.K. and D. Schlessinger. 1990. Mechanism and regulation of bacterial ribosomal RNA processing. Annu. Rev. Microbiol. 44: 105-129.
5. Sequences supplement. 1990. Nucleic Acids Res. 18.
6. Higgins, D.G. and P.M. Sharp. 1988. Clustal: A package for performing multiple sequence alignment on a microcomputer. Gene 73: 237-244.
7. Canard, B. and S.T. Coles. 1989. Genome organisation of the anaerobic pathogen C. perfringens. Proc. Natl. Acad. Sci. 86: 6676-6680.
8. Ennis, P.D., J. Zemmour, R.D. Slater, and P. Parham. 1990. Rapid cloning of HLA-A,B cDNA by using the polymerase chain reaction: Frequency and nature of errors produced in amplification. Proc. Natl. Acad. Sci. 87: 2833-2837.
9. Rogall, T., J. Wolters, T. Flohr, and E.C. Bottger. 1990. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int. J. Syst. Bacteriol. 40: 323-330.
10. Gardes, M., T.J. White, J.A. Fortin, T.D. Burns, and J.W. Taylor. 1991. Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae of nuclear and mitochondrial ribosomal DNA. Can. J. Bot. 69: 180-190.
11. Cato, E.P., W.L. George, and S.M. Finegold. 1986. The genus Clostridia. In Bergey's manual of systematic bacteriology (eds. P.H.A. Sneath, N.S. Mair, M.E. Sharpe, and J.G. Holt), pp. 1141-1200. Williams and Wilkins, Baltimore.
12. Minton, N.P. and D.J. Clarke. 1989. Clostridia. Biotechology handbooks 3. Plenum Press, New York and London.