Generation of a Lysosomal Enzyme Targeting Signal in the Secretory Protein Pepsinogen
of outstanding interest, Analysis of the phosphorylation of chimeric molecules between cathespin D and pepsinogen, a lysosomal and a non-lysosomal member of the aspartyl proteinase family. Demonstrates that two non-continuous sequences, which comprise less than 10% of the cathepsin D coding sequence, are required for phosphorylation of the chimeric molecule.
Quaternary Structure of the Mr 46000 Mannose 6-phosphate Specific Receptor: Effect of Ligand, pH and Receptor Concentration on the Equilibrium Between Dimeric and Tetrameric Receptor Forms
Rapid Equilibrium Between Monometic, Dimeric and Tetrameric Forms of the 46-kDa Mannose 6-Phosphate Receptor at 370C. Possible Relation to the Function of the Receptor
The Cation-Dependent Mannose 6-Phosphate Receptor Contains Two Internalization Signals in its Cytoplasmic Domain
of interest, Analysis of the internalization sequences of the bovine MPR46. Documents that two independent internalization sequences in the cytoplasmic tail of the receptor are required for rapid internalization, both of which contain critical aromatic residues.
Phosphorylation of the cytoplasmic domain of the bovine cation-independent mannose 6-phosphate receptor
of interest, Describes the phosphorylation of the cytoplasmic tail of MPR300 by a casein kinase II associated with the 47 kD subunits of the Golgi HA I adaptor complex.
A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole
of outstanding interest, Identifies the VPS15 gene product as a protein kinase which is essential for the targeting of soluble hydrolases to the yeast vacuole and is peripherally associated with the cytoplasmic face of the late Golgi or a vesicle compartment.
Distinctive regulation of the functional linkage between the human cation-independent mannose 6-phosphate receptor and GTP binding proteins by insulin-like growth factor II and mannose 6-phosphate
of outstanding interest, This report unequivocally identifies the human MPR300 as a G-protein-coupled receptor. IGF-II activates the G protein, while Man-6-P inhibits the IGF-II mediated activation.
Insulin-like growth factor-II/mannose 6-phosphate receptor is incapable of activating GTP-binding proteins in response to mannose 6-phosphate, but capable in response to Insulin-like growth factor-II
The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus
of outstanding interest, This report identifies the first mouse mutant in which MPR300 is deficient and the MPR300 gene as a maternally imprinted gene deficient in the Time mutant.
Targeting of a lysosomal membrane protein: a tyrosine-containing endocytosis signal in the cytoplasmic tail of lysosomal acid phosphatase is necessary and sufficient for targeting to lysosomes
of outstanding interest, Demonstrates that a Tyr-containing internalization sequence in the cytoplasmic tail of lysosomal acid phosphatase, a type I integral membrane protein is required for targeting to lysosomes. Its loss results in accumulation of newly synthesized lysosomal acid phosphatase at the cell surface.
Accumulation of membrane glycoproteins in lysosomes requires a tyrosine residue at a particular position in the cytoplasmic tail
of interest, Identifies a Tyr containing sorting signal in the cytoplasmic tail of the lysosmal membrane glycoprotein lamp 1, that is required for targeting to lysosomes.
A new class of lysosomal/vacuolar protein sorting signals
of interest, Describes the localization of the sorting signal for a type II integral membrane protein of yeast vacuoles in its cytoplasmic/transmembrane domain.
