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1
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0024490271
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High-Density Culture of Escherichia coli Carrying Recombinant Plasmid in a Membrane Cell Recycle Fermenter
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(1989)
Enzyme Microb Technol
, vol.11
, pp. 49-54
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Lee1
Gu2
Chang3
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3
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0024416873
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Control of Carbon Flux to Acetate Excretion During Growth of Escherichia coli in Batch and Continuous Cultures
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(1989)
J Gen Microbiol
, vol.135
, pp. 2875-2883
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El-Mansi1
Holms2
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4
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0025405421
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Simple Constrained-Optimization View of Acetate Overflow in E. coli
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Of interest, Production of acetate is formulated in terms of a flow network that has as its objective maximal ATP synthesis. It is found that when loads are imposed and flux constraints exist, either at the level of NADH turnover or the activity of a key Krebs cycle enzyme, switching to acetate overflow can be predicted.
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(1990)
Biotechnol Bioeng
, vol.35
, pp. 732-738
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Majewski1
Domach2
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6
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0025700668
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Production of Recombinant Human Growth Hormone in Escherichia coli Expression of Different Precursors and Physiological Effects of Glucose Acetate and Salts
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Of outstanding interest, Glucose fed-batch fermentations using complex media result in higher yields of Met-Ala-Glu-human growth hormone compared with batch fermentations in which the inhibitory effect of formed acetic acid is enhanced by a salt effect, caused by the increased consumption of acid and base as a means of controlling pH.
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(1990)
Biotechnology and Bioengineering
, vol.36
, pp. 1-11
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Bech1
Carisen2
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7
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0000689684
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Fermentation Technology Using Recombinant Microorganisms
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2 and heat transfer, and high-cell-density growth, with examples.
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(1989)
Biotechnology
, pp. 117-140
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Fieschko1
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10
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0000658485
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High Cell Density Culture of E. coli in a Fed-Batch System with Dissolved Oxygen as Substrate Feed Indicator
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(1989)
Biotechnol Lett
, vol.11
, pp. 155-160
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Cutayar1
Poillon2
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11
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84912856162
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Ausbeutesteigerung in der Fermentation Durch Computereinsatz
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Okt
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(1989)
Bio Tec
, pp. 65-67
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Kruger1
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15
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0002648640
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Hochzelldichte-Fermentationen von Escherichia coli für rDNA-Produkte
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2 limitation and significant accumulation of metabolic by-products. The specific growth rate can be adjusted such that product generation reaches its optimum value.
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(1990)
Bio Eng
, vol.5
, pp. 28-33
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Knorre1
Decjwer2
Korz3
Pohl4
Riesenberg5
Ross6
Sanders7
Schuiz8
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18
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0024459957
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Production of Human Leukocyte Interferon in Escherichia coli by Control of Growth Rate in Fed-Batch Fermentation
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(1989)
Biotechnol Lett
, vol.2
, pp. 695-698
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Lee1
Choi2
Kang3
Park4
Kwon5
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21
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0025314282
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The Production of Recombinant beta-galactosidase in Escherichia coli in Yeast Extract Enriched Medium
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Of interest, The productivity of E. coli biomass and recombinant β-galactosidase was increased in Luria broth far more by enrichment of the medium with yeast extract than by supplementation with various amounts of amino acids, vitamins, minerals, purines/pyrimidines, tryptone, casamino acids, casein peptone or gelatin peptone.
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(1990)
J Ind Microbiol
, vol.5
, pp. 85-94
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Li1
Robbins2
Taylor3
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22
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0025700264
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Glucose-Limited Fed-Batch Cultivation of Escherichia coli with Computer Controlled Fixed Growth Rate
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max) was achieved by a stepwise increase in glucose input. The pumping rate of the glucose-salt medium at any time was calculated from the amount of glucose fed into the fementer up to that time.
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(1990)
Biotechnol Bioeng
, vol.35
, pp. 312-319
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Paalme1
Tiisma2
Kahru3
Vanatalu4
Vilu5
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23
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84912893525
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Control of Intracellular Activities in Bioreactors
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Of outstanding interest, Methods for direct and indirect controls of specific growth rates are described on the basis of usual growth models and data of the off-gas composition from cultures in stirred tank reactors.
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(1990)
Bio Eng
, vol.6
, pp. 12-22
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Luttmann1
Stilkenbäumer2
Ross3
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27
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0025236410
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Effects of Medium Quality on the Expression of Human Interleukin-2 at High Cell Density in Fermentor Cultures of Escherichia coli K-12
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on outstanding interest, Medium perfusion to maintain low acetate concentrations leads to an improvement in specific IL-2 accumulation at high cell densities.
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(1990)
Appl Environ Microbiol
, vol.56
, pp. 640-645
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MacDonald1
Neway2
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28
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0025266044
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Effect of High Density Cultivation on Plasmid Copy Number in Recombinant Escherichia coli Cells
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Of interest, A high segregational and structural stability of vectors (e.g. pUR290) was observed during fed-batch cultivation to high cell densities in semisynthetic and synthetic media.
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(1990)
Biotechnol Lett
, vol.12
, pp. 191-196
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Horn1
Krug2
Sawistowski3
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29
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0025055526
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Use of Escherichia coli ssb Gene to Prevent Bioreactor take over by Plasmidless Cells
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Plasmids carrying the ssb gene are stably maintained in the E. coli host AB 1157 Δ. The plasmid stabilization method described for this amino acid auxotroph strain can also be applied to prototrophic or Thi auxotroph strains in HCDCs.
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(1990)
Biotechnology
, vol.8
, pp. 47-51
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Porter1
Black2
Pannuri3
Carlson4
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30
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0023231766
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The Effect of Organic Nitrogen and Glucose on the Production of Recombinant Human Insulin-Like Growth Factor in High Cell Density Escherichia coli Fermentations
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(1987)
J Ind Microbiol
, vol.2
, pp. 181-187
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Tsai1
Mann2
Morris3
Rotgers4
Fenton5
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34
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0025495561
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Gene Expression using Gram-Negative Bacteria
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Of outstanding interest, This review on secretion focuses on recent findings about the release of homologous and heterologous proteins into the periplasmic space and into the culture medium.
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(1990)
Curr Opin Biotechnol
, vol.1
, pp. 5-11
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Shatzman1
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36
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0024804726
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Production of Alkaline Phosphatase by Immobilized Growing Cells of Escherichia coli Excretory Mutants
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Of interest, In continuous cultures, alkaline phosphatase was excreted for more than 250h by immobilized growing cells. In contrast, excretion of this enzyme by free cells decreased after 150h of culture.
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(1989)
Appl Microbiol Biotechnol
, vol.32
, pp. 143-147
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Manin1
Barbotin2
Thomas3
Lazzaroni4
Portalier5
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38
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84912858401
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2 in the exhaust gas and culture mass.
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39
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84912860162
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International Minerals & Chemical Corp: Fermentation Process for the High Level Production of Swine Growth Hormone. 28/6/85 85 US-751976. 29/11/88 US 4788144., A high-density fermentation process for high yield production of swine growth hormone by transformant E. coli using complex media is described.
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40
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84912854521
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2 in the exit gas and by increasing the feed rate of glucose for the fermenter responsive to the calculated μ.
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41
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84912881758
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Institut für Mikrobiologie und Experimentelle Therapie: Optimization of Recombinant Gene Expression in Escherichia coli. 21/12/90 DD-WP C12N/338 0002., Modification of an expression unit with the cap sequence leads to enhanced formation of the respective recombinant gene product during glucose-limited growth in continuous cultures and fed-batch cultures.
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42
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84912855693
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Gesellschaft für Biotechnologische Forschung mbH und Institut Für Microbiologie und Experimental Therapie: Method for Expression of Recombinant Genes Under Conditions of Limitations of Nitrogen Source. 31/1/91 DE-P 41002594-6., Recombinant genes linked with a promoter that is similar to the glutamine synthetase promoter 2 can be expressed in E. coli under conditions of nitrogen source shortage.
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