Purification and characterization of cyclosporine and FK-506 binding proteins from a human T-helper cell line

Clinical Biochemistry

Volumn 24, Issue 1, 1991, Pages 63-70

  Palaszynski, Edmund W ^a,b   Donnelly, James G ^a,b   Soldin, Steven J ^a,b  

^a CHILDREN'S NATIONAL MEDICAL CENTER   (United States)

^b GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

cyclosporine; FK 506 binding protein; immune suppression; mechanism of action; protein purification

Indexed keywords

CYCLOSPORIN; DRUG BINDING PROTEIN; TACROLIMUS;

CONFERENCE PAPER; HELPER CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN PURIFICATION;

AMINO ACID ISOMERASES; ANTIBIOTICS, MACROLIDE; CARRIER PROTEINS; CELL LINE; CHROMATOGRAPHY, LIQUID; CYCLOSPORINS; CYTOSOL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMAN; MOLECULAR WEIGHT; PEPTIDYLPROLYL ISOMERASE; RADIOLIGAND ASSAY; SUPPORT, NON-U.S. GOV'T; T-LYMPHOCYTES, HELPER-INDUCER; TACROLIMUS;

EID: 0026071896     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/0009-9120(91)90252-A     Document Type: Article

References (24)

1. Cyclosporine: historical perspectives
2. Cyclosporine: clinical applications and monitoring methods
3. The effect of cyclosporine on the use of hospital resources for kidney transplantation
4. Metabolic and immunological effects of cyclosporine in recently diagnosed type 1 diabetes mellitus
5. Cyclosporine improves psoriasis in a double-blind study
6. Treatment of severe vernal keratoconjunctivitis with cyclosporine A eyedrops
7. FK-506 a novel immunosuppressant isolated from a Streptomyces
8. Novel immunosuppressive agent, FK-506
9. Effect of a new immunosuppressive agent, FK-506, on heterotopic cardiac allotransplantation in the rat
10. Pathological studies in canine baboon renal allograft recipients immunosuppressed with FK-506
11. FK-506 dosage in human organ transplantation
12. The effects of FK-506 on renal function after liver transplantation
13. Cyclophilin: a specific binding protein for cyclosporine A
14. FK-506, a potent novel immunosuppressive agent binds to a cytosolic protein which is distinct from the cyclosporine A binding protein, cyclophilin
15. Cyclophilin and peptidyl-prolyl cis-trans isomerase are probably identical proteins
16. A receptor for the immunosuppressant FK-506 is a cis-trans peptidyl-prolyl isomerase
17. A radioreceptor assay for the measurement of cyclosporine activity: a preliminary report
18. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
19. Cleavage of structural proteins during assembly of the head of bacteriophage T4
20. Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining
21. A cytosolic binding protein for the immunosuppressant FK-506 has peptidyl-prolyl isomerase activity but is distinct from cyclophilin
22. The immunosuppressant FK-506 selectively inhibits expression of early T-cell activation genes
23. Peptidyl-prolyl cis-trans isomerase is the cyclosporine A binding protein cyclophilin
24. The attraction of proteins for small molecules and ions