A gentle fixation and permeabilization method for combined cell surface and intracellular staining with improved precision in DNA quantification

Cytometry

Volumn 12, Issue 3, 1991, Pages 279-285

  Schmid, Ingrid ^a   Uittenbogaart, Christel H ^a   Giorgi, Janis V ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

7 AAD; Flow cytometry; internal antigens; propidium iodide

Indexed keywords

7 AMINODACTINOMYCIN; DNA; MEMBRANE ANTIGEN; PROPIDIUM IODIDE;

ARTICLE; CELL LINE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MAMMAL CELL; MEMBRANE PERMEABILITY; PRIORITY JOURNAL;

ANTIGENS; ANTIGENS, SURFACE; BLOOD CELLS; CELL MEMBRANE PERMEABILITY; COMPARATIVE STUDY; CYTOPLASM; DACTINOMYCIN; DNA; FIXATIVES; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; FORMALDEHYDE; HUMAN; POLYMERS; POLYSORBATES; PROPIDIUM; STAINING AND LABELING; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

MAMMALIA; MURINAE;

EID: 0026061995     PISSN: 01964763     EISSN: 10970320     Source Type: Journal    
DOI: 10.1002/cyto.990120312     Document Type: Article

References (24)

1. Monoclonal antibodies reactive with the T cell receptor chain: Production and characterization using a new method
2. A method of simultaneous nuclear Immunofluorescence and DNA content using monoclonal antibodies and flow cytometry
3. Microfluorometric investigations of chromatin structure. I. Evaluation of nine DNA‐specific fluorochromes as probes of chromatin organization
4. Methods and applications of flow systems for analysis and sorting of mammalian cells
5. Solubilization of membranes by detergents
6. Selective solubilization with Tween 20 of membrane proteins from Acholeplasma laidlawii
7. Characterization of a leukemic cell line of the pre‐B phenotype
8. Preparation, staining and analysis by flow cytometry of peripheral blood leukocytes
9. Cell cycle expression of nuclear proteins
10. Rapid cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
11. Paraformaldehyde fixation of hematopoetic cells for quantitative flow cytometry (FACS) analysis
12. Flow cytometric analysis of human bone marrow. I. Normal erythroid development
13. Lymphocyte proliferation
14. Immunofluorescence quantification of ribonucleotide reductase M1 subunit and correlation with DNA content by flow cytometry
15. A unique human B lymphocyte antigen defined by a monoclonal antibody
16. On the chemistry of formaldehyde fixation and its effect on immunohistochemical reactions
17. Simultaneous cell cycle analysis and two‐color surface immunofluorescence using 7‐amino‐actinomycin D and single laser excitation,: Applications to study of cell activation and the cell cycle of murine Ly‐1 B cells
18. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system
19. A flow‐cytometric method for the quantitative analysis of intracellular and surface membrane antigens
20. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T cell malignancies
21. Conversion of logarithmic channel numbers into relative linear fluorescence intensity
22. Growth of human malignant lyphoid cell‐lines in serum free medium
23. Enumeration of cytoplasmic m̈ immunoglobulin positive Acute Lyphoblastic Leukemia cells by flow cytometry: Comparison with fluorescence microscopy