Purification of human respiratory syncytial virus: superiority of sucrose gradient over percoll, renografin, and metrizamide gradients

Journal of Virological Methods

Volumn 31, Issue 2-3, 1991, Pages 161-170

  Mbiguino, Angĺique ^a   Menezes, José ^a  

^a UNIVERSITY OF MONTREAL   (Canada)

Author keywords

Concentration; Gelatin bead; Purification; Respiratory syncytial virus

Indexed keywords

DIATRIZOATE; METRIZAMIDE; PERCOLL; SUCROSE; UNCLASSIFIED DRUG;

ARTICLE; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; VIRUS CULTURE; VIRUS PURIFICATION;

CARCINOMA; CELL LINE; CENTRIFUGATION, DENSITY GRADIENT; COMPARATIVE STUDY; CULTURE MEDIA; DIATRIZOATE MEGLUMINE; HUMAN; LARYNGEAL NEOPLASMS; METRIZAMIDE; OSMOTIC PRESSURE; POVIDONE; RESPIRATORY SYNCYTIAL VIRUSES; SILICON DIOXIDE; SUPPORT, NON-U.S. GOV'T; VIRUS ACTIVATION; VISCOSITY;

EID: 0026028044     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(91)90154-R     Document Type: Article

References (19)

1. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). II. Epidemiologic aspects of infection in infants and young children
2. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization
3. Biophysical studies of respiratory syncytial virus. I. Density of respiratory syncytial virus and associated complement-fixing antigens in cesium chloride density gradient
4. 4
5. Biophysical studies of respiratory syncytial virus. II. Identification of two soluble complement-fixing antigens of respiratory syncytial virus
6. Separation of clonogenic cells from stationary phase cultures by density gradient centrifugation
7. Buoyant density separation of human blood cells in renografin gradients
8. Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine
9. Polypeptides of respiratory syncytial virus
10. Thermal inactivation of respiratory syncytial virus in water and hypertonic solutions
11. Metrizamide, a new density gradient medium
12. Separation and concentration of respiratory syncytial virus (RSV) antigens
13. Contribution a l'étude des protéines structurales du virus syncytial respiratoire humain
14. A simple and rapid microassay for the titration of human respiratory syncytial virus
15. Concentration of human respiratory syncytial virus using ammonium sulfate, polyethylene glycol or hollow fiber ultrafiltration
16. Respiratory syncytial virus. I. Concentration and purification of the infectious virus
17. Respiratory syncytial virus proteins
18. Negative strand viruses
19. Stabilizing effect of inorganic salts on RS virus