Expression and enzymatic activity of recombinant cytochrome P450 17α-hydroxylase in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America

Volumn 88, Issue 13, 1991, Pages 5597-5601

  Barnes, H J ^a   Arlotto, M P ^a   Waterman, M R ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

bacterial expression; reductase; steroid hydroxylase

Indexed keywords

CYTOCHROME P450; RECOMBINANT PROTEIN; STEROID 17ALPHA MONOOXYGENASE;

ARTICLE; CATTLE; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; CATTLE; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA; ESCHERICHIA COLI; HYDROXYPREGNENOLONE; IN VITRO; MOLECULAR SEQUENCE DATA; PRASTERONE; PREGNENOLONE; PROGESTERONE; RECOMBINANT PROTEINS; SPECTRUM ANALYSIS; STEROID 17-ALPHA-HYDROXYLASE; SUPPORT, U.S. GOV'T, P.H.S.;

BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; ESCHERICHIA COLI; EUKARYOTA; MAMMALIA;

EID: 0025994649     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.88.13.5597     Document Type: Article

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