The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest

Proceedings of the National Academy of Sciences of the United States of America

Volumn 88, Issue 21, 1991, Pages 9578-9582

  Chien, Cheng Ting ^a   Bartel, Paul L ^a   Sternglanz, Rolf ^a   Fields, Stanley ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Dimerization; GAL4; Transcriptional activation; Transcriptional silencing; Yeast

Indexed keywords

MAMMALIA;

ARTICLE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; TRANSCRIPTION REGULATION; YEAST;

CLONING, MOLECULAR; FUNGAL PROTEINS; GENETIC VECTORS; GENOMIC LIBRARY; MACROMOLECULAR SYSTEMS; NUCLEAR PROTEINS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; SACCHAROMYCES CEREVISIAE; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0025940629     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.88.21.9578     Document Type: Article

References (28)

1. Lane, D. P. & Crawford, L. V. (1979) Nature (London) 278, 261-263.
2. DeCaprio, J. A., Ludlow, J. W., Figge, J., Shew, J.-Y., Huang, C.-M., Lee, W.-H., Marsilio, E., Paucha, E. & Livingston, D. M. (1988) Cell 54, 275-283.
3. Nelbock, P., Dillon, P. J., Perkins, A. & Rosen, C. A. (1990) Science 248, 1650-1653.
4. Fields, S. & Song, O. (1989) Nature (London) 340, 245-246.
5. Keegan, L., Gill, G. & Ptashne, M. (1986) Science 231, 699-704.
6. Rine, J. & Herskowitz, I. (1987) Genetics 116, 9-22.
7. Gill, G. & Ptashne, M. (1987) Cell 51, 121-126.
8. Thomas, B. J. & Rothstein, R. (1989) Cell 56, 619-630.
9. Sherman, F., Fink, G. R. & Hicks, J. B. (1986) Methods in Yeast Genetics (Cold Spring Harbor Lab., Cold Spring Harbor, NY).
10. Schiestl, R. H. & Gietz, R. D. (1989) Curr. Genet. 16, 339-346.
11. Hall, M. N., Hereford, L. & Herskowitz, I. (1984) Cell 36, 1057-1065.
12. Benton, B. M., Eng, W.-K., Dunn, J. J., Studier, F. W. Sternglanz, R. & Fisher, P. A. (1990) Mol. Cell. Biol. 10, 353-360.
13. Ma, J. & Ptashne, M. (1987) Cell 48, 847-853.
14. Ammerer, G. (1983) Methods Enzymol. 101, 192-201.
15. Ma, J. & Ptashne, M. (1987) Cell 51, 113-119.
16. Mullen, J. R., Kayne, P. S., Moerschell, R. P., Tsunasawa, S., Gribskov, M., Colavito-Shepanski, M., Grunstein, M., Sherman, F. & Sternglanz, R. (1989) EMBO J. 8, 2067-2075.
17. Kimmerly, W. J. & Rine, J. (1987) Mol. Cell. Biol. 7, 4225-4237.
18. Celenza, J. L., Eng, F. J. & Carlson, M. (1989) Mol. Cell. Biol. 9, 5045-5054.
19. Huffman, C. S. & Winston, F. (1987) Gene 57, 267-272.
20. Laughon, A. & Gesteland, R. F. (1984) Mol. Cell. Biol. 4, 260-267.
21. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467.
22. Silver, P. A., Keegan, L. P. & Ptashne, M. (1984) Proc. Natl. Acad. Sci. USA 81, 5951-5955.
23. Kalderon, D., Roberts, B. L., Richardson, W. D. & Smith, A. E. (1984) Cell 39, 499-509.
24. Nelson, M. & Silver, P. (1989) Mol. Cell. Biol. 9, 384-389.
25. Alberts, B. M. & Sternglanz, R. (1990) Nature (London) 344, 193-194.
26. Marshall, M., Mahoney, D., Rose, A., Hicks, J. B. & Broach, J. R. (1987) Mol. Cell. Biol. 7, 4441-4452.
27. Diffley, J. F. X. & Stillman, B. (1989) Nature (London) 342, 24.
28. Miller, J. H. (1972) Experiments in Molecular Genetics (Cold Spring Harbor Lab., Cold Spring Harbor, NY).