Trace analysis of methotrexate and 7-hydroxymethotrexate in human plasma and urine by a novel high-performance liquid chromatographic method

Therapeutic Drug Monitoring

Volumn 13, Issue 6, 1991, Pages 528-532

  Beck, Olof ^a   Seideman, Peter ^b   Wennberg, Mona ^a   Peterson, Curt ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b DANDERYD HOSPITAL   (Sweden)

Author keywords

7 Hydroxymethotrexate; Fluorometric detection; High performance liquid chromatography; Methotrexate; Photolytic oxidation; Plasma; Solid phase extraction; Urine

Indexed keywords

7 HYDROXYMETHOTREXATE; METHOTREXATE;

ARTICLE; DRUG ANALYSIS; DRUG BLOOD LEVEL; DRUG URINE LEVEL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLUOROMETRY; HUMAN; METHOTREXATE; MICROCHEMISTRY;

EID: 0025787690     PISSN: 01634356     EISSN: 15363694     Source Type: Journal    
DOI: 10.1097/00007691-199111000-00011     Document Type: Article

References (16)

1. Bleyer WA. The clinical pharmacology of methotrexate: new applications of an old drug. Cancer 1978;41:36-51.
2. Kimura K, Wang YM. Methotrexate in cancer therapy. New York: Raven Press, 1986:213-23.
3. Watson E, Cohen JL, Chan K. High-pressure liquid chromatographic determination of methotrexate and its major metabolite, 7-hydroxymethotrexate, in human plasma. Cancer Treat Rep 1978;62:381-7.
4. Cairnes DA, Evans WE. High-performance liquid chromatographic assay of methotrexate, 7-hydroxymethotrexate, 4-deoxy-4-amino-N10-methylpteroic acid and sulfamethoxazole in serum, urine and cerebrospinal fluid. J Chromatogr1982;231:103-10.
5. El-Yazigi A, Martin CR. Rapid determination of methotrexate and 7-hydroxymethotrexate in serum and cerebrospinal fluid by radial compression liquid chromatography. J Liq Chromatogr 1984;7:1579-91.
6. So N, Chandra DP, Alexander IS. Determination of serum methotrexate and 7-hydroxymethotrexate concentrations. J Chromatogr 1985;337:81-90.
7. Stout M, Ravindranath Y, Kauffman R. High performance liquid chromatographic assay for methotrexate utilizing cold acetonitrile purification and separation of plasma or cerebrospinal fluid. J Chromatogr 1985;342:424-30.
8. Slordal L, Prytz PS, Pattersen I, Aarbakke J. Methotrexate measurement in plasma: comparison of enzyme multiplied immunoassay technique, TDx fluorescence polarization immunoassay, and high pressure liquid chromatography. Ther Drug Monit 1986;8:368-72.
9. Brimmel PA, Sams DJ. Rapid and simple assay for the measurement of methotrexate in serum, urine and blood cells by reversed phase high-performance liquid chromatography. J Chromatogr 1987;413:320-5.
10. Alkaysi HN, Gharaibeh AM, Sheikh Salem MA. High-performance liquid chromatographic determination of methotrexate in plasma. Ther Drug Monit 1990;12:191-4.
11. Erttman R, Bielack S, Landbeck G. Kinetics of 7-hydroxymethotrexate after high-dose methotrexate therapy. Cancer Chemother Pharmacol 1985;15:101-4.
12. Lankelma J, Van der Klein E, Ramaekers F. The role of 7-hydroxy-methotrexate during methotrexate anticancer therapy. Cancer Lett 1980;9:133-42.
13. Wilke SW, Mackenzie AH. Methotrexate therapy in rheumatoid arthritis: current status. Drugs 1986;32:103-13.
14. Salamoun J, Frantisek J. Determination of methotrexate and its metabolites 7-hydroxymethotrexate and 2,4-diamino-N10-methylpteroic acid in biological fluids by liquid chromatography with fluorimetric detection. J Chromatogr 1986;378: 173-81.
15. Salamoun J, Smrz M, Kiss F, Salamounova A. Column liquid chromatography of methotrexate and its metabolites using a post-column photochemical reactor and fluorescence detection. J Chromatogr 1987;419:213-23.
16. Dixit V, Dixit VM. A unique solid phase extraction column for isolation of 11 A9- nor-A9-tetrahydrocannabinol-9-carboxylic acid in human urine. J Liquid Chromatogr 1990; 13:3313-25.