Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli

Gene

Volumn 100, Issue C, 1991, Pages 195-199

  Rong Fu Wang, ^a   Kushner, Sidney R ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

ampicillin resistance; deletion analysis; kanamycin resistance; polymerase chain reaction; Recombinant DNA; single stranded DNA

Indexed keywords

RECOMBINANT DNA;

ARTICLE; CLONING VECTOR; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE;

AMPICILLIN RESISTANCE; BASE SEQUENCE; CLONING, MOLECULAR; COLIPHAGES; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC MARKERS; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; PLASMIDS; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0025782890     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(91)90366-J     Document Type: Article

References (20)

1. Nucleotide sequence of the gene ompA encoding the outer membrane protein II of Escherichia coli K-12
2. Complete sequence of pSC101
3. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection
4. Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12
5. Construction of biologically functional bacterial plasmids in vitro
6. Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on the specialized transducing phages λtna
7. Unidirectional digestion with exonuclease III create targets breakpoints for DNA sequencing
8. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K and RK2
9. Preparation of single-stranded DNA from phagemids
10. Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability
11. Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering
12. Direct sequencing of denatured plasmid DNA
13. Characterization of the Escherichia coli polA gene
14. Primer-directed enzymatic DNA amplification of DNA with a thermostable DNA polymerase
15. λZAP: a bacteriophage A expression vector with in vivo excision properties
16. Defective and plaque-forming lambda transducing bacteriophage carrying pencillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome
17. Versatile low-copy-number plasmid vectors for cloning in Escherichia coli
18. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
19. The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers
20. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors