Engineering Subtilisin and Its Substrates for Efficient Ligation of Peptide Bonds in Aqueous Solution

Biochemistry

Volumn 30, Issue 17, 1991, Pages 4151-4159

  Abrahms, Lars ^a   Tom, Jeffrey ^a   Burnier, John ^a   Butcher, Karen A ^a   Kossiakoff, Anthony ^a   Wells, James A ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; SUBTILISIN;

ARTICLE; BIOENGINEERING; ENZYME SPECIFICITY; ENZYME SUBSTRATE; PRIORITY JOURNAL;

AMINES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; PEPTIDES; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SUBTILISINS;

EID: 0025757012     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi00231a007     Document Type: Article

References (47)

1. Barany, G., & Merrifield, R. B. (1980) in The Peptides (Gross, E., & Meienhoffer, J., Eds.) Vol 2, pp 1–284, Academic Press, New York.
2. Barbas, C. F., III, Matos, J. R., West, J. B., & Wong, C.-H. (1988) J. Am. Chem. Soc. 110, 5162–5166.
3. Bott, R., Ultsch, M., Kossiakoff, A., Graycar, T., Katz, B., & Power, S. (1988) J. Biol. Chem. 263, 7895–7906.
4. Brünger, A. T. (1990) X-PLOR Manual, Version 2.1, Yale University, New Haven, CT.
5. Bryan, P. N., Rollence, M. L., Pantoliano, N. W., Wood, J., Finzel, B. C., Gilliland, G. L., Howard, A. J., & Poulos, T. L. (1986) Proteins: Struct., Fund., Genet. 1, 326–334.
6. Caldwell, R., Graycar, T., Ultsch, M., Graham, F., Estell, D., & Bott, R. (1989) J. Cell. Biochem. 13A, 51 (suppl.).
7. Carter, P., & Wells, J. A. (1987) Science, 237, 394–399.
8. Carter, P., & Wells, J. A. (1988) Nature 332, 564–568.
9. Carter, P., Bedouelle, H., & Winter, G. (1986) Nucleic Acids Res. 13, 4431–4443.
10. Carter, P., Nilsson, B., Burnier, J., Burdick, D., & Wells, J. A. (1989) Proteins: Struct., Fund., Genet. 6, 240–248.
11. Chaiken, I. M. (1981) CRCCrit. Rev. Biochem. 11, 255–301.
12. Chu, S. H., & Mautner, H. B. (1966) J. Org. Chem. 31, 308.
13. Coletti-Previero, M.-A., Previero, A., & Zuckerkandl, E. (1969) J. Mol. Biol. 39, 493–501.
14. Cunningham, B. C., & Wells, J. A. (1987) Protein Eng. 1, 319–325.
15. Ellman, G. L. (1959) Arch. Biochem. Biophys. 82, 70–77.
16. Estell, D. A., Graycar, T. P., & Wells, J. A. (1985) J. Biol. Chem. 260, 6518–6521.
17. Estell, D. A., Graycar, T. P., Miller, J. V., Powers, D. B., Burnier, J. P., Ng, P. G., & Wells, J. A. (1986) Science 233, 659–663.
18. Inouye, K., Watanabe, K., Morihara, K., Tochino, Y., Kanaya, T., Empura, J., & Sakakibara, S. (1979) J. Am. Chem. Soc. 101, 751–752.
19. Jones, T. A. (1978) J. Appl. Crystallogr. 11, 268–272.
20. Kabsch, W. (1988) J. Appl. Crystallogr. 21, 916–934.
21. Kaiser, E. T. (1989) Acc. Chem. Res. 22, 47–54.
22. Kettner, C. A., & Shenvi, A. B. (1984) J. Biol. Chem. 259, 15106–15114.
23. Kullmann, W. (1987) in Enzymatic peptide synthesis, CRC Press, Boca Raton, FL.
24. McPhalen, C. A., & James, M. N. G. (1988) Biochemistry 27, 6582–6598.
25. Messerschmidt, A., & Pflugrath, J. W. (1987) J. Appl. Crystallogr. 20, 306–315.
26. Morihara, K., & Oka, T. (1977) Arch. Biochem. Biophys. 178, 188–194.
27. Morihara, K., Oka, T., & Tsuzuki, H. (1970) Arch. Biochem. Biophys. 138, 515–525.
28. Nakatsuka, T., Sasaki, T., & Kaiser, E. T. (1987) J. Am. Chem. Soc. 109, 3808–3810.
29. Offord, R. E. (1987) Protein Eng. 1, 151–157.
30. Pantoliano, M. W., Whitlow, M., Wood, J. F., Dodd, S. W., Hardman, K. D., Rollence, M. L., & Bryan, P. N. (1989) Biochemistry 28, 7205–7213.
31. Pauling, L. (1960) in The Nature of the Chemical Bond, 3rd ed., pp 264–360, Cornell University Press, Ithaca, NY.
32. Perry, K. M., Fauman, E. B., Finer-Moore, J. S., Montfort, W. R., Maley, G. F., Maley, F., & Stroud, R. M. (1990) Proteins: Struct., Fund., Genet. 8, 315–333.
33. Philipp, M., & Bender, M. L. (1983) Mol. Cell. Biochem. 51, 5–32.
34. Power, S. D., Adams, R. M., & Wells, J. A. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 3096–3100.
35. Riechmann, L., & Kasche, V. (1984) Biochem. Biophys. Res. Commun. 120, 686–691.
36. Riechmann, L., & Kasche, V. (1985) Biochim. Biophys. Acta 830, 164–172.
37. Robertus, J. D., Alden, R. A., Birktoft, J. J., Kraut, J., Powers, J. C., & Wilcox, P. E. (1972) Biochemistry 11, 2439–2449.
38. Sanger, F., Nicklen, S., & Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463–5467.
39. Schechter, I., & Berger, A. (1967) Biochem. Biophys. Res. Commun. 27, 157–162.
40. Wells, J. A. (1990) Biochemistry 29, 8509–8517.
41. Wells, J. A., Ferrari, E., Henner, D. J., Estell, D. A., & Chen, E. Y. (1983) Nucleic Acids Res. 11, 7911–7925.
42. Wells, J. A., Cunningham, B. C., Graycar, T. P., & Estell, D. A. (1986) Philos. Trans. R. Soc. London, A 317, 415–423.
43. Wells, J. A., Powers, D. B., Bott, R. R., Graycar, T. P., & Estell, D. A. (1987a) Proc. Natl. Acac. Sci. U.S.A. 84, 1219–1223.
44. Wells, J. A., Cunningham, B. C., Graycar, T. P., & Estell, D. A. (1987b) Proc. Natl. Acad. Sci. U.S.A. 84, 5167–5171.
45. Wong, C. -H., Chen, S. -T., Hennen, W. J., Bibbs, J. A., Wang, Y. -F., Liu, J. L. -C., Pantoliano, M. W., Whitlow, M., & Bryan, P. N. (1990) J. Am. Chem. Soc. 112, 945–953.
46. Wu, Z.-P., & Hilvert, D. (1989) J. Am. Chem. Soc. III, 4513–4514.
47. Yang, M. Y., Ferrari, E., & Henner, D. J. (1984) J. Baderiol. 160, 15–21.