A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry

Journal of Immunological Methods

Volumn 139, Issue 2, 1991, Pages 271-279

  Nicoletti, I ^a   Migliorati, G ^a   Pagliacci, M C ^a   Grignani, F ^a   Riccardi, C ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Apoptosis; Dexamethasone; DNA fragmentation; Flow cytometry; Thymocyte

Indexed keywords

DEXAMETHASONE;

ANIMAL CELL; ARTICLE; CELL DEATH; CONTROLLED STUDY; FLOW CYTOMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; THYMOCYTE;

ANIMAL; CELL SURVIVAL; CYCLOHEXIMIDE; DEXAMETHASONE; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; FLOW CYTOMETRY; MICE; PROPIDIUM; SPECTROMETRY, FLUORESCENCE; STAINING AND LABELING; SUPPORT, NON-U.S. GOV'T; THYMUS GLAND;

ANIMALIA;

EID: 0025726216     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(91)90198-O     Document Type: Article

References (15)

1. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
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4. Activation of a suicide process of thymocytes through DNA fragmentation by calcium ionophores and phorbol esters
5. Calcium-activated DNA fragmentation kills immature thymocytes
6. Simultaneous cell surface phenotype and cell cycle analysis of lymphocytes by flow cytometry
7. Protein kinase-C involvement in thymocyte apoptosis induced by hydrocortisone
8. Gene induction by γ-irradiation leads to DNA fragmentation in lymphocytes
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10. Antibodies to CD3/T-cell receptor complex induce death by apoptosis in immature T cells in thymic cultures
11. Monoclonal antibody-mediated tumor regression by induction of apoptosis
12. Haemopoietic colony stimulating factors promote cell survival by suppressing apoptosis
13. Cell death: the significance of apoptosis
14. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis