An ancient group I intron shared by eubacteria and chloroplasts

Science

Volumn 250, Issue 4987, 1990, Pages 1570-1573

  Kuhsel, Maria G ^a   Stkickland, Roderick ^a   Palmer, Jeffrey D ^a  

^a INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); CYANOBACTERIA;

LEUCINE TRANSFER RNA;

ALGA; ANTICODON; ARTICLE; CHLOROPLAST; COMPARATIVE STUDY; CONFORMATION; CYANOBACTERIUM; EUBACTERIUM; EVOLUTION; GENETICS; INTRON; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT; POLYMERASE CHAIN REACTION;

ALGAE; ANTICODON; BASE SEQUENCE; CHLOROPLASTS; CLONING, MOLECULAR; COMPARATIVE STUDY; CYANOBACTERIA; EUBACTERIUM; EVOLUTION; INTRONS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PLANTS; POLYMERASE CHAIN REACTION; RNA, TRANSFER, LEU; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0025707121     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.2125748     Document Type: Article

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27. PCR products were purified by preparative electrophoresis, cloned into the Eco RV site of phagemid Bluescript SK+, and sequcnced by the dideoxy chain termination method. Five independent clones (resulting from one to three independent PCR amplifications) were sequenced for each species, and both strands of each product were sequenced. Except for the primer-binding regions, all five clones were identical for any one species. The GenBank accession numbers of the nine intron sequences are M36884 to M36886, M36888 to M36890, M36892, M36893, and M55288.
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38. We thank R. Butow, J. Logsdon, R. Olmstead, N. Pace, P. Perlman, D. Shub, K. Wolfe, and M. Zolan for comments on the manuscript; D. Shub for communication of manuscript (21) prior to submission; D. Gilbert for providing the program "Loop-D-Loop"; A. Grossman for Anacystis DNA and library; and A. Coleman, S. Fain, L. Goff, R. Meints, K. Kowallik, N. Pace, S. Turner, A. Wilmotte, and K. Zetsche for DNA's and cell pellets. Supported by NIH grant 35087 (J.D.P.) and Deutsche Forschungunsgemeinschaft grant KU721/1-1 (M.G.K.).