Assessment of Fura-2 for measurements of cytosolic free calcium

Cell Calcium

Volumn 11, Issue 2-3, 1990, Pages 63-73

  Roe, M W ^a   Lemasters, J J ^a   Herman, B ^a,b  

^a Duke University Trent Drive   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FURA 2;

ARTICLE; CALCIUM CELL LEVEL; METHODOLOGY; PRIORITY JOURNAL;

ANIMAL; BENZOFURANS; CALCIUM; CYTOSOL; FLUORESCENT DYES; FURA-2; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0025260882     PISSN: 01434160     EISSN: None     Source Type: Journal    
DOI: 10.1016/0143-4160(90)90060-8     Document Type: Article

References (45)

1. The role of calcium in cell death
2. 2+ homoeostasis in hepatocytes
3. 2+ messenger system (parts a and b)
4. 2+ messenger system (parts a and b)
5. Regional changes in calcium underlying contraction of single smooth muscle cells
6. Fura-2 diffusion and its use as an indicator of transient free calcium changes in single striated muscle cells
7. Fura-2 transients in frog skeletal muscle fibres
8. Epidermal growth factor stimulates the rapid accumulation of inositol (1,4,5)-trisphosphate and a rise in cytosolic free calcium mobilized from intracellular stores in A431 cells
9. Platelet-derived growth factor and angiotensin II cause increases in cytosolic free calcium by different mechanisms in vascular smooth muscle cells
10. Calcium-induced assembly of adherens junctions in keratinocytes
11. 2+-induced phospholipid domains
12. Fluorescent probes of cell signalling
13. 2+ indicators with greatly improved fluorescence properties
14. Calcium homoeostasis in intact lymphocytes: Cytoplasmic free calcium monitored with a new intracellularly trapped fluorescent indicator
15. 2+ with trapped indicators
16. Fluorescence ratio imaging: a new window into intracellular ionic signalling
17. 2+-insensitive form of Fura-2 associated with polymorphonuclear leukocytes
18. 2+ in monolayers and suspensions of various types of animal cells
19. Incomplete hydrolysis of the calcium indicator Fura-2 pentaacetoxymethyl ester (Fura-2/AM) by cells
20. Aequorin entrapment in mammalian cells
21. Chloral hydrate disrupts mitosis by increasing intracellular free calcium
22. 2+ by receptors
23. 2+ oscillations induced by hormonal stimulation of individual Fura-2 loaded hepatocytes
24. 2+-sensitive forms by mitochondria
25. Fura-2 fluorescence is localized to mitochondria in endothelial cells
26. 1 Adrenergic stimulation and cytoplasmic free calcium concentration in cultured renal proximal tubular cells: Evidence for compartmentalization of Quin-2 and Fura-2
27. 2+ exchange in cultured embryonic heart cells
28. Intracellular pH during ‘chemical hypoxia’ in cultured rat hepatocytes: protection by intracellular acidosis against the onset of cell death
29. Fluorescence probe measurement of the intralysomal pH in living cells and the perturbation of pH by various agents
30. Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria spectral and metabolic properties
31. Rapid separation of particulate components and soluble cytoplasm of isolated liver cells
32. Free calcium transients in spontaneously beating rat neonatal myocytes measured by digitized video microscopy
33. Probenecid inhibits Fura-2 secretion and sequestration in the mouse macrophage cell line J774
34. Microspectrofluorometry by digital image processing: measurement of cytoplasmic pH
35. Platelet activating factor raises intracellular calcium ion concentrations in macrophages
36. Photobleaching of Fura-2 and its effects on determination of calcium concentrations
37. Changes in free calcium levels with stages of the cell division cycle
38. 2+ homeostasis in Erlich and Yoshida carcinomas. A new, membrane permeant chelator of heavy metals reveals that these ascites tumor cell lines have normal cytosolic free calcium
39. 2+ homoeostasis in Erlich and Yoshida carcinomas
40. An assessment of the use of Fura-2 for the determination of intracellular calcium concentrations
41. The Ca signal from Fura-2 loaded mast cells depends strongly on the method of dye-loading
42. 2+ in individual small cells using fluorescent microscopy with dual excitation wavelengths
43. Diffusion of injected macromolecules within the cytoplasm of living cells
44. Myoplasmic binding of Fura-2 investigated by steady-state fluorescence and absorbance measurements
45. 2+ indicator precursor Fura-2/AM