The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae

Cell

Volumn 63, Issue 4, 1990, Pages 835-841

  Xu, Gangfeng ^a   Lin, Boris ^b   Tanaka, Kazuma ^b   Dunn, Diane ^a   Wood, Douglas ^b   Gesteland, Ray ^a   White, Ray ^a   Weiss, Robert ^a   Tamanoi, Fuyuhiko ^b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MAMMALIA; SACCHAROMYCES CEREVISIAE;

GENE PRODUCT; GUANOSINE TRIPHOSPHATASE;

ARTICLE; DNA SEQUENCE; NEUROFIBROMATOSIS; ONCOGENE RAS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

CLONING, MOLECULAR; COMPARATIVE STUDY; ESCHERICHIA COLI; FUNGAL PROTEINS; GENES, FUNGAL; GENES, RAS; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; HUMAN; NEUROFIBROMATOSIS 1; NEUROFIBROMIN 1; PLASMIDS; PROTEINS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0025244911     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(90)90149-9     Document Type: Article

References (26)

1. Guanosine triphosphate activating protein (GAP) interacts with the p21 ras effector binding domain
2. Genetic analysis of mammalian GAP expressed in yeast
3. ras genes
4. The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product
5. A major segment of the neurofibromatosis type 1 gene: cDNA sequence, genomic structure, and point mutations
6. High efficiency transformation of E. coli by high voltage electroporation
7. ras upon T-cell activation
8. Enzymatic amplification of specific cDNA inserts from λ-gt11 libraries
9. Purification of ras GTPase-activating protein from bovine brain
10. PDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex
11. Binding of GAP to activated PDGF receptors
12. A C-terminal domain of GAP is sufficient to stimulate ras p21 GTPase activity
13. ras GTPase activating protein: signal transmitter and signal terminator
14. PDGF induction of tyrosine phosphorylation of GTPase activating protein
15. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
16. Neurofibromatosis
17. Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I
18. IRA1, an inhibitory regulator of the RAS/cyclic AMP pathway in Saccharomyces cerevisiae
19. IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein
20. S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein
21. IRA2, an upstream negative regulator of RAS in yeast, is a RAS GTPase-activating protein (GAP)
22. A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants
23. Deletions and a translocation interrupt a cloned gene at the neurofibromatosis type 1 locus
24. Type 1 neurofibromatosis gene: identification of a large transcript disrupted in three NF1 patients
25. The neurofibromatosis type 1 gene encodes a protein related to GAP