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Volumn 250, Issue 4980, 1990, Pages 562-565

Molecular cloning and functional expression of the cardiac sarcolemmal Na+-Ca2+ exchanger

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DOG; HEART MUSCLE FIBER MEMBRANE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SODIUM CALCIUM EXCHANGE; XENOPUS LAEVIS;

EID: 0025243512     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1700476     Document Type: Article
Times cited : (629)

References (32)
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    • Oxford Univ. Press, Oxford
    • T. J. A. Allen, D. Noble, H. Reuter, Sodium-Calcium Exchange (Oxford Univ. Press, Oxford, 1989); K. D. Philipson, in Calcium and the Heart, G. A. Langer, Ed. (Raven, New York, 1990), pp. 85-108.
    • (1989) Sodium-Calcium Exchange
    • Allen, T.J.A.1    Noble, D.2    Reuter, H.3
  • 2
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    • G. A. Langer, Ed. Raven, New York
    • T. J. A. Allen, D. Noble, H. Reuter, Sodium-Calcium Exchange (Oxford Univ. Press, Oxford, 1989); K. D. Philipson, in Calcium and the Heart, G. A. Langer, Ed. (Raven, New York, 1990), pp. 85-108.
    • (1990) Calcium and the Heart , pp. 85-108
    • Philipson, K.D.1
  • 3
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    • W. J. Lederer, E. Niggli, R. W. Hadley, Science 248, 283 (1990); N. Leblanc and J. R. Hume, ibid., p. 372; J. H. B. Bridge, J. R. Smolley, K. W. Spitzer, ibid., p. 376.
    • (1990) Science , vol.248 , pp. 283
    • Lederer, W.J.1    Niggli, E.2    Hadley, R.W.3
  • 4
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    • W. J. Lederer, E. Niggli, R. W. Hadley, Science 248, 283 (1990); N. Leblanc and J. R. Hume, ibid., p. 372; J. H. B. Bridge, J. R. Smolley, K. W. Spitzer, ibid., p. 376.
    • Science , pp. 372
    • Leblanc, N.1    Hume, J.R.2
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    • W. J. Lederer, E. Niggli, R. W. Hadley, Science 248, 283 (1990); N. Leblanc and J. R. Hume, ibid., p. 372; J. H. B. Bridge, J. R. Smolley, K. W. Spitzer, ibid., p. 376.
    • Science , pp. 376
    • Bridge, J.H.B.1    Smolley, J.R.2    Spitzer, K.W.3
  • 8
    • 0023461432 scopus 로고
    • The λgt11 canine ventricle cDNA library was screened with the polyclonal antibody, and DNA from positive phage was isolated by standard techniques [M. Snyder, S. Elledge, D. Sweetser, R. A. Young, R. W. Davis, Methods Enzymol. 154, 107 (1987); T. V. Huynh, R. A. Young, R. W. Davis, in DNA Cloning (IRL Press, Oxford, 1984), vol. 1, pp. 49-78].
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    • Snyder, M.1    Elledge, S.2    Sweetser, D.3    Young, R.A.4    Davis, R.W.5
  • 9
    • 0001903367 scopus 로고
    • IRL Press, Oxford
    • The λgt11 canine ventricle cDNA library was screened with the polyclonal antibody, and DNA from positive phage was isolated by standard techniques [M. Snyder, S. Elledge, D. Sweetser, R. A. Young, R. W. Davis, Methods Enzymol. 154, 107 (1987); T. V. Huynh, R. A. Young, R. W. Davis, in DNA Cloning (IRL Press, Oxford, 1984), vol. 1, pp. 49-78].
    • (1984) DNA Cloning , vol.1 , pp. 49-78
    • Huynh, T.V.1    Young, R.A.2    Davis, R.W.3
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    • 32P (Oligolabeling Kit, Pharmacia), and libraries were screened by standard procedures [W. D. Benton and R. W. Davis, Science 196, 180 (1977)]. Plasmids in the vector Bluescript SK were prepared by the automatic excision procedure of the manufacturer (Stratagene).
    • (1987) Anal. Biochem. , vol.162 , pp. 156
    • Chomczynski, P.1    Sacchi, N.2
  • 12
    • 0015356037 scopus 로고
    • 32P (Oligolabeling Kit, Pharmacia), and libraries were screened by standard procedures [W. D. Benton and R. W. Davis, Science 196, 180 (1977)]. Plasmids in the vector Bluescript SK were prepared by the automatic excision procedure of the manufacturer (Stratagene).
    • (1972) Proc. Natl. Acad. Sci. U.S.A. , vol.69 , pp. 1408
    • Aviv, H.1    Leder, P.2
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    • 0017367397 scopus 로고
    • 32P (Oligolabeling Kit, Pharmacia), and libraries were screened by standard procedures [W. D. Benton and R. W. Davis, Science 196, 180 (1977)]. Plasmids in the vector Bluescript SK were prepared by the automatic excision procedure of the manufacturer (Stratagene).
    • (1977) Science , vol.196 , pp. 180
    • Benton, W.D.1    Davis, R.W.2
  • 14
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    • note
    • The TB11 plasmid was digested with Bam HI and RNA was synthesized with T7 RNA polymerase following the instructions of the manufacturer (Bethesda Research Laboratories).
  • 17
    • 0020009435 scopus 로고
    • The peptide, acetyl-DDKQPLTSKEEEERR-amide, was synthesized by Multiple Peptide Services. The peptide was coupled to keyhole limpet hemocyanin with glutaraldehyde, emulsified with complete Freund's adjuvant, and injected subcutaneously at multiple sites along the back of a rabbit (about 0.4 mg of peptide per rabbit). Three weeks later, this procedure was repeated with coupled peptide and incomplete Freund's adjuvant. Rabbits were bled through the ear artery at approximately 1-month intervals thereafter. ELISA assays were performed as described [D. S. Papermaster, Methods Enzymol. 81, 240 (1982)].
    • (1982) Methods Enzymol. , vol.81 , pp. 240
    • Papermaster, D.S.1
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    • C. Sardet, A. Franchi, J. Pouysségur, Cell 56, 271 (1989); K. E. Kudrycki, P. R. Newman, G. E. Shull, J. Biol. Chem. 265, 462 (1990).
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    • Sardet, C.1    Franchi, A.2    Pouysségur, J.3
  • 27
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    • We noted that this region was cationic and resembled a calmodulin-binding peptide [K. T. O'Neil and W. F. DeGrado, Trends Biochem. Sci. 15, 59 (1990)]. A peptide corresponding to residues 251 to 270 was synthesized and was found to bind calmodulin (J. T. Penniston and K. D. Philipson, unpublished observations).
    • (1990) Trends Biochem. Sci. , vol.15 , pp. 59
    • O'Neil, K.T.1    DeGrado, W.F.2
  • 28
    • 0025159272 scopus 로고    scopus 로고
    • unpublished observations
    • We noted that this region was cationic and resembled a calmodulin-binding peptide [K. T. O'Neil and W. F. DeGrado, Trends Biochem. Sci. 15, 59 (1990)]. A peptide corresponding to residues 251 to 270 was synthesized and was found to bind calmodulin (J. T. Penniston and K. D. Philipson, unpublished observations).
    • Penniston, J.T.1    Philipson, K.D.2
  • 32
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    • note
    • We thank the members of the laboratory of E. Wright for many helpful discussions, D. Woo for advice on the production of antipeptide antibodies, B. Scott for the λgt11 library, R. Ward for technical assistance, and E. Wright and H. R. Kaback for commenting on the manuscript. Supported by NIH HL27821, the Laubisch Foundation, and NIH National Research Service Award training grant AHL07386.


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